The invention relates to the field of
DNA amplification, in particular to an intelligent molecular diagnosis and
analysis method, device and equipment and a storage medium. The method comprises the following steps: detecting multi-dimensional environmental parameters of the
DNA amplification kit in real
time based on a multi-dimensional sensing node; performing spatial topology
layout mining and environment amplification
perception fitting on the multi-dimensional environment parameters, and constructing a real-time amplification
perception model; identifying
a DNA amplification sample; performing layered
temperature control analysis on the
DNA amplification sample, and performing dynamic amplification constant-
temperature control on the real-time amplification
perception model, so as to construct a plurality of dynamic constant-temperature amplification models; carrying out regional DNA reaction degree evaluation on the plurality of dynamic isothermal amplification models one by one, and then carrying out regional flow velocity dynamic regulation, so as to construct a dynamic amplification reaction flow velocity regulation strategy; real-time amplification control is performed based on a dynamic amplification reaction flow rate adjustment strategy to generate a filter optimized amplification reaction
signal. According to the method, the accuracy of molecular diagnosis analysis is improved through efficient and convenient DNA isothermal amplification.