The invention relates to the technical field of antibacterial liquid, and in particular relates to an antibacterial liquid used for nasopharyal meatus and a preparation method thereof. The antibacterial liquid per liter is prepared from the following raw materials: 1.7 to 1.9g of dialkyl quaternary ammonium salt, 30 to 60ml of glycerinum, 0.1 to 1.3g of menthol, 0.9 to 3.0g of sodium chloride, 5 to 8g of extracts of traditional Chinese medicine, and the balance of purified water. The antibacterial liquid adopts a plurality of raw materials capable of clearing heat, dispelling wind, relieving sore throat, resisting inflammation, resisting bacteria and easing pain; all the raw materials supplement each other and develop the synergism to realize the characteristics of the best antibacterial performance, short treatment course and quick return of the antibacterial liquid. The biocompatibility of the antibacterial liquid meets the requirement of GB / T 16886.11-2011, and the microbiological indicator meets the requirement of GB15979-2002. The antibacterial liquid is applicable to control and nursing of nasal infection, oral infection, throat infection, various acute (chronic) rhinitis, pharyngitis, auditory meatus inflammation, dental ulcer, and skin and mucous membrane wound infection, and can be used for promoting the healing of the wound and reducing the postoperative infection.