Method for measuring bleomycins by utilizing electrochemical luminescence of carbon nanoparticle modified electrode

A technology of carbon nanoparticles and modified electrodes, which is applied in the direction of chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, etc., and can solve problems such as stability defects

Inactive Publication Date: 2013-12-11
广州谱峰科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

However, due to the deficiencies of antibodies in their production, product stability, and clinical operations, people are seeking their substitutes

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  • Method for measuring bleomycins by utilizing electrochemical luminescence of carbon nanoparticle modified electrode
  • Method for measuring bleomycins by utilizing electrochemical luminescence of carbon nanoparticle modified electrode
  • Method for measuring bleomycins by utilizing electrochemical luminescence of carbon nanoparticle modified electrode

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Abstract

The invention relates to a method for detecting the content of bleomycins (BLMs), belonging to the technical fields of electrochemical luminescence and sensors. According to the method, an electrochemical luminescence agent is utilized for marking DNA (deoxyribonucleic acid) to prepare a probe (Ru-DNA), and a carboxylated carbon nanoparticle modified electrode is utilized for constructing a carboxylated carbon nanoparticle modified electrode electrochemical luminescence biosensor through a competitive effect between an electrostatic repulsion force and a pi-pi accumulation action on the basis of non-covalent-bond self-assembly principles of the DNA and the carbon nanoparticle. Target objects, namely the bleomycins, act on the DNA probes to cut the DNA, the DNA probes adsorbing on the carbon nanoparticle modified electrode are reduced, and weak electrochemical luminescence signals are generated, thereby realizing the measurement of the bleomycins. The method has the advantages of simplicity and rapidness.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and electrochemiluminescence sensors, in particular to a method and application for measuring bleomycin by electrochemiluminescence of a carbon nanoparticle-modified electrode. technical background [0002] For modern biochemical and biomedical research, sensitive and accurate measurement of disease-related proteins is crucial in many aspects, especially for the detection and accurate prediction of early-stage characteristic proteins of cancer. Immunoassay is a powerful analytical assay technique that has been widely used in the detection and analysis of tumor markers. Antibodies are used as recognition elements due to their high specificity and strong recognition. However, due to the deficiencies of antibodies in their production, product stability, and clinical operations, people are seeking their substitutes. [0003] DNA biosensor is a sensing device that can convert the target that inter...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 混旭刘芳
Owner 广州谱峰科技有限公司
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