The invention provides a method and a detection kit for identifying
ovarian cancer biomarkers. The biomarkers are free glucose and free
mannose which are obtained by subjecting serum to pre-column 3-methyl-1-phenyl-2-pyrazolin-5-one (PMP)
derivatization high-performance liquid
chromatography. A detection method is a pre-column PMP
derivatization high-performance liquid
chromatography. According tothe technical scheme, the method and the detection kit for identifying the
ovarian cancer biomarkers have the advantages that the pre-treatment is simple, analysis time is short, the instrument priceis reasonable, the conventional use is met, the operation steps are simple and easy to learn, the detection result is high in accuracy, a normal person and an
ovarian cancer patient can be distinguished by just sampling blood and subjecting the blood to the high-performance liquid
chromatography for analysis, moreover, the required serum amount is very few, the amount of sampled blood is less than 1 mL, and the like. An obtained result shows that by means of the
analysis method, the free
mannose and glucose in the serum of the ovarian
cancer patient can be fast quantified, so that the methodand the detection kit for identifying the ovarian
cancer biomarkers have significant meaning to studying a relation between the free
mannose and the free glucose in the serum and ovarian
cancer and finding a novel ovarian cancer clinical detection marker.