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A kind of mycoplasma bovis culture medium and preparation method

A technology of mycoplasma bovis and culture medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, pharmaceutical formulations, etc., to achieve the effects of inhibiting the growth of bacteria, promoting microbial metabolism, and promoting protein synthesis

Active Publication Date: 2019-08-13
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, since the above-mentioned patents are used to cultivate Mycoplasma hyopneumoniae and Mycoplasma capricum subspecies, the titers of live bacteria are not higher than 10 when culturing Mycoplasma bovis. 9 CCU / ml. In addition, the amount of horse serum required by the above-mentioned patents is relatively large, and the serum content is about 10% to 20%. Therefore, at this stage, research on a medium that can efficiently culture Mycoplasma bovis and can use less serum has been carried out. It has become an important problem to be solved urgently in the research and production of Mycoplasma bovis vaccine

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  • A kind of mycoplasma bovis culture medium and preparation method

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Embodiment 1

[0029] 1, the preparation of culture medium of the present invention:

[0030] Basal medium:

[0031] (1) Friis media premix 22.0g

[0032] (2) Sodium pyruvate 5.0 g

[0033] (3) Glucose 6.0g

[0034] (4) 2.0 ml of phenol red with a mass concentration of 1%

[0035] (5) 750 ml of deionized water.

[0036] Auxiliary medium:

[0037] (6) 110 ml of fresh yeast extract with a mass concentration of 25%

[0038] (7) 17.0 ml of L-glutamine with a mass concentration of 3%

[0039] (8) 5.0 ml of L-cysteine ​​with a mass concentration of 10%

[0040] (9) 33.0 ml of hydrolyzed milk protein with a mass concentration of 15%

[0041] (10) Transferrin (10 mg / ml) 1.0 ml

[0042] (11) Insulin (10 mg / ml) 1.0 ml

[0043] (12) Penicillin (200,000 IU / ml) 0.25ml

[0044] (13) Sterile horse serum 50 ml

[0045]Dissolve the ingredients (1) to (4) in the basic medium into 750ml deionized water (5) one by one, and autoclave at 115°C for 20 minutes. After cooling to room temperature, under ...

Embodiment 2

[0051] The preparation of substratum of the present invention:

[0052] Basal medium:

[0053] (1) Friis media premix 22.0 g

[0054] (2) Sodium pyruvate 5.0 g

[0055] (3) Glucose 8.0 g

[0056] (4) 2.0 ml of phenol red with a mass concentration of 1%

[0057] (5) 750ml of deionized water.

[0058] Auxiliary medium:

[0059] (6) 120 ml of fresh yeast extract with a mass concentration of 25%

[0060] (7) 17.0 ml of L-glutamine with a mass concentration of 3%

[0061] (8) 5.0 ml of L-cysteine ​​with a mass concentration of 10%

[0062] (9) 33.0 ml of hydrolyzed milk protein with a mass concentration of 15%

[0063] (10) Transferrin (10 mg / ml) 1.0 ml

[0064] (11) Insulin (10 mg / ml) 1.0 ml

[0065] (12) Penicillin (200,000 IU / ml) 0.25ml

[0066] (13) Sterile horse serum 50 ml

[0067] Dissolve the components (1)-(4) in the basic medium into 750ml deionized water (5) one by one, and autoclave at 115°C for 20min. After cooling, under aseptic conditions, add auxiliary ...

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Abstract

The invention discloses a mycoplasma bovis medium and a preparation method thereof. The mycoplasma bovis medium comprises a basic medium and an auxiliary medium through mixing and is prepared from Friis media premix, sodium pyruvate, a fresh yeast extract liquid, glucose, phenol red, deionized water, L-glutamine, L-cysteine, lactoalbumin hydrolysate, transferrin, insulin, penicillin and a small amount of horse serum. The medium not only can reduce the use amount of serum, but also remarkably increases the titer of living bacteria of mycoplasma bovis and lays a foundation for preparation of high quality vaccines of mycoplasma bovis.

Description

technical field [0001] The invention relates to a mycoplasma culture medium and a preparation method thereof, specifically, a mycoplasma bovis whose components include: sodium pyruvate, fresh yeast extract, glucose, phenol red, deionized water, penicillin and a small amount of horse serum Culture medium and its preparation method. Background technique [0002] Mycoplasma bovis ( Mycoplasma bovis, M.bovis ) can cause bovine pneumonia, arthritis, mastitis, keratoconjunctivitis, otitis, reproductive tract inflammation, miscarriage and infertility and other diseases. The disease caused by this pathogen is prevalent all over the world and causes serious economic losses. Mycoplasma bovis is the main cause of bovine respiratory disease syndrome. Since Mycoplasma bovis was first isolated from beef cattle with outbreaks of necrotizing pneumonia in Hubei Province in 2008, there have been reports of cases of Mycoplasma bovis in many provinces and cities across the country. Now Mycop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04A61P11/00C12R1/35
CPCA61K39/0241C12N1/20
Inventor 陈胜利储岳峰郝华芳赵萍刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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