A kind of low-serum high-efficiency culture medium of ovine mycoplasma pneumoniae and preparation method thereof

Abstract

The invention discloses a lower-serum and efficient culture medium of mycoplasma ovipneumoniae. The lower-serum and efficient culture medium is prepared from the following components: MEM, sodium pyruvate, glucose, Hank's liquid, fresh yeast leaching liquid, L-glutamine, L-cysteine, lactalbumin hydrolysate, calf thymus DNA (Deoxyribonucleic Acid), insulin, transferrin, penicillin, horse serum, phenol red and de-ionized water; the invention provides a preparation method of the lower-serum and efficient culture medium. The lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, has the beneficial effects that the content of the serum is only 5 percent and is 1 / 4 of the content of the serum in a culture medium in the prior art; the titer of mycoplasma ovipneumoniae semi-finished-product bacterium liquid prepared by the method reaches 10<10>CCU / ml and is obviously higher than that of the culture medium in the prior art. According to the lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, the sensitive stress response to a goat body, caused by heterologous serum, is alleviated, and the biosafety is improved; the titer of bacterium liquid of living bacteria is also improved and the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of veterinary preparations, in particular to a low serum high-efficiency culture medium of Mycoplasma ovine pneumonia and a preparation method thereof. Background technique [0002] Mycoplasma ovipneumoniae (Mo) can cause interstitial pneumonia in sheep and goats. The clinical features are mainly cough, asthma, runny nose, weight loss, anemia, and growth retardation. In 1963, Mackay et al first isolated Mycoplasma ovine pneumonia from the lung of Scottish sheep disease. Mycoplasma ovine pneumonia mainly infects sheep and goats. At present, the incidence of sheep and goats caused by Mycoplasma ovine pneumonia has been reported in many countries around the world, causing huge economic losses to the world sheep industry. In 1978, Chinese scholars isolated Mycoplasma ovine pneumonia for the first time in Sichuan Province from the offspring lambs of the Border Region Lester sheep introduced from New Zealand. Su...

AI Technical Summary

Problems solved by technology

There are problems such as long culture time, low titer of live bacteria and poor reproductive ability in culturing Mycoplasma pneumoniae in the culture medium of the prior art

In addition, the serum content in the culture medium of the prior art is generally 20%. Vaccines prepared with high serum content will undoubtedly increase the allergic stress response of the heterologous serum to the sheep body, which will eventually affect the immune effect of the vaccine and increase the cost of vaccine production.

If the serum content in the culture medium of the prior art is simply reduced, the antigen titer of Mycoplasma ovis will be lowered by about 10 to 100 times, and the antigen dose required for immunization will not be reached, and the concentration multiple needs to be increased, which actually increases the production cost.

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