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Serum copper, iron and zinc rapid sensitive detection kit and preparing method thereof

A sensitive detection and kit technology, applied in the field of rapid and sensitive detection kits, can solve the problems of affecting the results, many interference factors, poor sensitivity, etc., and achieve the effect of single temperature setting of reagents, fast and easy operation, and high sensitivity

Inactive Publication Date: 2005-07-06
浙江东瓯诊断产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is simple and fast, but the sensitivity is poor and there are many interference factors, especially iron pollution is the most serious, and hemolysis will also affect the results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A serum copper rapid and sensitive detection kit, the kit is composed of the following materials:

[0028] Oxidant (30g / L hydrogen peroxide) 1 bottle

[0029] Chromogenic solution (pyridylazo chromogenic reagent) 1 bottle

[0030] Copper standard solution (concentration is 31.48μmol / L) 1 bottle

[0031] The preparation method of this kit is realized through the following steps:

[0032] (1) 30g / L hydrogen peroxide is configured in a conventional method;

[0033] (2) The preparation method of pyridylazo chromogenic solution:

[0034] a. Weigh 200 mg of 2-(5-bromo-2-pyridylazo)-5-(diethylaminophenol),

[0035] b. Make the reagent in a into a 100ml stock solution with absolute ethanol,

[0036] c. Take 5 ml of the stock solution of b, add 10 g of polyoxyethylene isooctyl phenyl ether and 20 g of citric acid, and dilute to 1000 ml with acetic acid-sodium acetate aqueous solution of pH 4.0;

[0037] (3) Copper standard solution: Accurately weigh 100.0mg of metallic cop...

Embodiment 2

[0043] A serum copper rapid and sensitive detection kit, the kit is composed of the following materials:

[0044] Oxidant (30g / L potassium persulfate) 1 bottle

[0045] Chromogenic solution (pyridylazo chromogenic reagent) 1 bottle

[0046] Copper standard solution (concentration is 15.74μmol / L) 1 bottle

[0047] The preparation method of this kit is realized through the following steps:

[0048] (1) Potassium persulfate of 30g / L is configured in a conventional method

[0049] (2) The preparation method of pyridylazo color developer:

[0050] a. Weigh 100 mg of 2-(3,5-dibromo-2-pyridylazo)-5-diethylaminophenol,

[0051] b. Make the reagent in a into a 100ml stock solution with absolute ethanol,

[0052] c. Take 50ml of the stock solution of b, add 20g of polyoxyethylene lauryl ether and 50g of sodium fluoride, and dilute to 1000ml with acetic acid-sodium acetate aqueous solution of pH 3.8.

[0053] (3) Copper standard solution: Accurately weigh 100.0 mg of metallic coppe...

Embodiment 3

[0059] A serum zinc rapid and sensitive detection kit, the kit is composed of the following reagents:

[0060] Chromogenic solution (pyridylazo chromogenic reagent) 1 bottle

[0061] Zinc standard solution (concentration is 15.3μmol / L) 1 bottle

[0062] The preparation method of this kit is realized through the following steps:

[0063] (1) The preparation method of pyridylazo chromogenic solution:

[0064] a. Weigh 300 mg of 2-(5-bromo-2-pyridylazo)-5-dimethylaminophenol,

[0065] b. Make the reagent in a into a 100ml stock solution with absolute ethanol,

[0066] c. Take 30ml of the stock solution of b, add 15g of polyoxyethylene sorbitan lauric acid monoester and 20g of copper iron reagent, and dilute to 1000ml with tris hydrochloride aqueous solution with pH 8.0.

[0067] (2) Zinc standard solution: Accurately weigh 100.0 mg of metallic zinc (99.9%), dissolve it with an appropriate amount of 50% nitric acid solution and dilute to 1000 ml with double distilled water, th...

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Abstract

This invention discloses a test agent box to measure the contents of copper, iron and zinc in blood serum and provides the process method of the agent box. The agent box in this invention dissociates the protein with copper, iron and zinc in blood serum in the buffer liquid system with the surface activity agent, which is compound with the pyridylazo organic compound to make color. The color complex compound has the longest wavelength at 550-600nm.

Description

technical field [0001] The invention relates to rapid and sensitive detection kits respectively used for detecting the contents of copper, iron and zinc in blood serum and preparation methods of the respective kits. Background technique [0002] Copper, iron, and zinc are essential trace elements for the human body. [0003] Elevated serum copper is seen in leukemia, Hodgkin's disease, pigmentation, tuberculosis, pernicious anemia, and malignant tumors. Decreased serum copper is seen in infant anemia, infant malnutrition, Menkes curly hair syndrome, hepatolenticular degeneration and so on. There are many methods for measuring serum copper. At present, the preferred clinical method is atomic absorption spectrophotometry, which is adopted by most foreign laboratories. However, this method is expensive and difficult to popularize in clinical laboratories in my country. It is a spectrophotometric method, and the first method of the spectrophotometric method is to use dicyclohex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78G01N33/52G01N33/84
Inventor 曹建明
Owner 浙江东瓯诊断产品有限公司
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