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Kit for testing acute coronary artery complex by insulin growth factor combing protein-4 hydrolase

A growth factor and protein-binding technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of insufficient detection sensitivity of unstable angina pectoris and affect the accuracy of detection results, so as to reduce the amount of serum used and detect sensitive Increased speed and easy operation

Inactive Publication Date: 2006-07-26
国家人口和计划生育委员会出生缺陷干预工程技术中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently clinically used myocardial injury protein markers mainly include troponin I (Tn I) and troponin T (Tn T), although their specificity and sensitivity are significantly better than enzymatic indicators, but due to the Proteins are easily degraded by proteases in blood, thus affecting the accuracy of test results
And neither TnI nor TnT are sensitive enough to detect unstable angina

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: The use example of the production method of IGFBP-4 proteolytic enzyme quantitative enzyme immunoassay kit

[0039] IGFBP-4 proteolytic enzyme quantitative enzyme immunoassay kit (48 parts), its composition includes:

[0040] 1 bottle of IGFBP-4 proteolytic enzyme freeze-dried standard;

[0041] IGFBP-4 proteolytic enzyme polygram antibody-coated plate (48 wells) 1 piece;

[0042] Horseradish peroxidase (HRP)-labeled IGFBP-4 proteolytic enzyme monoclonal antibody 1 bottle, 6ml / bottle;

[0043] Freeze-dried low-value quality control product 1 bottle;

[0044] Freeze-dried high-value quality control product 1 bottle;

[0045] 1 bottle of substrate solution, 3ml / bottle;

[0046] Chromogenic solution (TMB) 1 bottle, 3ml / bottle;

[0047] 1 bottle of reaction termination solution, 3ml / bottle;

[0048] 1 bottle of washing buffer (20X concentrated), 15ml / bottle.

[0049] The specific operation is as follows:

[0050] 1. Preparation of IGFBP-4 proteolytic enzy...

Embodiment 2

[0079] Example 2: Example of use of IGFBP-4 proteolytic enzyme quantitative ELISA kit

[0080] 1. Washing buffer preparation: dilute the concentrated washing buffer provided in the kit with distilled water 20 times.

[0081] 2. Dissolve the first-point lyophilized powder (concentration: 64mIU / L) of the standard provided in the kit with 500 μl of washing buffer, and then perform doubling dilutions 4 times. The standard curve in the kit is composed of 5 different concentrations of IGFBP-4 proteolytic enzyme standards, the concentrations of each point of the standard curve are 64mIU / L, 32mIU / L, 16mIU / L, 8mIU / L, 4mIU / L.

[0082] 3. Dilution of quality control products: Dissolve the lyophilized powders of low and high quality control products provided in the kit with 110 μl of washing buffer respectively.

[0083] 4. Antigen-antibody reaction: Add 100 μl of diluted standard substance, quality control substance, or serum sample to be tested into the microwells of the antibody-coate...

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PUM

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Abstract

A reagent kit is composed of standard products, multiple clone antibody encapsulated plate, enzyme single clone antibody, quality control productand auxiliary reagent. The present invention also provides preparation method of kit and inspection method. The reaction method of quantitative biantibody sandwich enzyme linked immunosorbent is applied by the kit.

Description

technical field [0001] The invention belongs to the field of medical and biological detection, in particular, the invention relates to a detection of acute coronary syndrome by using insulin growth factor binding protein-4 (i.e. Insuline Growth Factor Binding Protein-4, referred to as IGFBP-4) hydrolase The kit can be used for the diagnosis of acute coronary syndrome, especially for the assessment of the risk of acute myocardial infarction, and the invention also relates to a preparation method and a measurement method of the kit. Background technique [0002] Acute Coronary Syndrome (ACS) has a high mortality rate in European and American countries, and it has a tendency to increase significantly in my country in recent years. ACS refers to the pathological phenomenon of atherosclerotic plaque shedding, platelet aggregation, and thrombus formation, resulting in coronary artery stenosis and blockage, myocardial ischemia and myocardial infarction. Clinical manifestations can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/535G01N33/96
Inventor 马旭吴尔若
Owner 国家人口和计划生育委员会出生缺陷干预工程技术中心
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