A method of simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol in aquatic products

A technology of medroxyprogesterone acetate and diethylstilbestrol, applied in the field of bioengineering, can solve the problems of high cost, time-consuming, single detection of veterinary drug residues and the like

Inactive Publication Date: 2013-07-17
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C +2
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It aims to solve the problems of single, time-consuming, and high-cost veterinary drug residue detection, and also lays the foundation for further simultaneous detection of four veterinary drug residue indicators

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method of simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol in aquatic products
  • A method of simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol in aquatic products
  • A method of simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol in aquatic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Preparation of medroxyprogesterone acetate artificial antigen.

[0059] Medroxyprogesterone acetate is coupled with ovalbumin to form an artificial antigen.

Embodiment 2

[0060] Preparation and purification of embodiment 2 medroxyprogesterone acetate monoclonal antibody

[0061] Medrgestrol acetate-3-(O-carboxymethyl)(MPS) hapten was synthesized by O-(carboxymethyl)hydroxylamine method. Medrgestrol acetate-3-(O-carboxymethyl) was coupled with BSA by dicyclohexylcarbodiimide coupling method to synthesize artificial complete immunogen. Four BALB / C mice were used for the first immunization with MPA-BSA and an equal volume of complete Freund's adjuvant emulsified and injected into the foot pads, the dose was 100ug / mouse, and then boosted immunization once every 3 weeks, and replaced with incomplete Freund's adjuvant. Adjuvant was injected subcutaneously, intramuscularly, and intraperitoneally at multiple points in sequence, with a dose of 150ug / carcass. Booster immunization once 3 days before the fusion, the tail vein of the mice was slowly and directly injected with MPA-BSA 250ug / mouse. Cell fusion was carried out according to conventional metho...

Embodiment 3

[0062]Example 3 Preparation of Diethylstilbestrol Artificial Antigen

[0063] Diethylstilbestrol was coupled with ovalbumin to form an artificial antigen.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of bio-engineering techniques, and relates to a suspension array method of simultaneous detection of medroxyprogesterone acetate and diethylstilbestrol in aquatic products. The method comprises the following steps: (1) preparing artificial antigens of medroxyprogesterone acetate and diethylstilbestrol individually; (2) preparing and purifying monoclonal antibodies against medroxyprogesterone acetate and diethylstilbestrol; (3) conjugating suspension array fluorescent microspheres to the artificial antigens obtained in step (1) separately; (4) biotinylating monoclonal antibodies obtained in step (2); and (5) simultaneously detecting medroxyprogesterone acetate and diethylstilbestrol residues in aquatic products by using conjugated fluorescent microspheres obtained in step (3) and biotinylated antibodies obtained in step (4) through the suspension array method. . The method provided by the invention can simultaneously detect two indicators of medroxyprogesterone acetate and diethylstilbestrol residues, and is fast, sensitive, and highly specific; wherein the minimum detection limits of both medroxyprogesterone acetate and diethylstilbestrol are 0.4ng/ml.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for simultaneously detecting medroxyprogesterone acetate and diethylstilbestrol in aquatic products by a liquid phase chip method. Background technique [0002] Medroxyprogesterone acetate (MPA) is a kind of progesterone drug, which is used as a growth-promoting agent in veterinary medicine. It has a wide range of toxic and side effects on people, and can cause adverse reactions such as mental depression and allergies, and even cause breast cancer and infertility. Member states of the European Union have already strictly prohibited the use in food-borne animal feeding and management. The Ministry of Agriculture of my country issued the "Maximum Residue Limits of Veterinary Drugs in Animal Foods" in 2002. Cannot be detected in food. Diethylstilbestrol (DES) is a non-steroidal hormone that has been widely used to promote animal growth and increase lean meat percentag...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 李健王艳陈沁王权陈政晓蒋蔚
Owner SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap