Immune chip for detecting staphylococcal enterotoxin and papaverine and its preparing method

Abstract

The present invention discloses an immune chip for detecting staphylococcal enterotoxin and papaverina dn its preparation process. The immune chip has slide glass with surface treated with silane as aldehydation reagent and crosslinked with glutaraldehyde as double-function crosslinking reagent, and connected via covalent bond with the molecule of at least one kind of antibody staphylococcal enteriotoxin A antibody, staphylococcal enterotoxin B antibody, staphylococcal enterotoxin C antibody and papaverine antibody. The detection with the immune chip is simple, effective and low in cost.

Description

technical field

[0001] The invention relates to an immune chip and a preparation method thereof, in particular to an immune chip for detecting staphylococcus enterotoxin and papaverine and a preparation method thereof. Background technique

[0002] Biological immune chip refers to the high-density antigen or antibody microarray coated on the solid phase carrier, which is a new type of biological chip after the gene chip. The immune chip is composed of antigen or antibody microarrays immobilized on different types of support media. The position and composition of the immobilized molecules in the array are known. The antigen reacts with the recognition molecules on the chip, and then is detected by a specific scanning device, and the results are analyzed and processed by a computer. Compared with traditional techniques for detecting contaminants in food, immunochips have many advantages. Traditional detection methods can only detect one pollu...