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Immune chip for detecting staphylococcal enterotoxin and papaverine and its preparing method

A Staphylococcus gut and immune chip technology, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems that it is difficult to meet multiple detections, improve the inability to perform high-throughput detection, save detection costs, and improve the former. The effect of cumbersome processing

Inactive Publication Date: 2003-06-25
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies can only detect one or one type of pollutants at a time, and the pollutants in food are unknown, sometimes multiple pollutants exist at the same time, the above methods will hardly meet the needs of multiple detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Prepare a glass slide having a surface cross-linked with a bifunctional cross-linking reagent glutaraldehyde after being silane-formylated with an aldehyde-forming reagent;

[0028] 2. Extract staphylococcal enterotoxin type A antibody, staphylococcal enterotoxin type B antibody, staphylococcal enterotoxin type C antibody and papaverine antibody;

[0029] 3. Antibody fixation, which includes:

[0030] ①Dilute Staphylococcal enterotoxin type A antibody with pH 7.2 phosphate buffer to a concentration of 1.0mg / ml Dilute staphylococcal enterotoxin type B antibody to a concentration of 0.5mg / ml Dilute staphylococcal enterotoxin type C Antibody, make the concentration 0.5mg / ml, dilute the papaverine antibody to make the concentration 1mg / ml;

[0031] ② Use a spotting instrument to drop the four antibody dilutions onto different areas of the slide surface, place at 37°C and saturated humidity for 0.5 hours, rinse with pH 7.2 phosphate buffer, then rinse with distilled wate...

Embodiment 2

[0034] Step 1 and step 2 are the same as embodiment 1.

[0035] 3. Antibody fixation, which includes:

[0036] ①Dilute Staphylococcal enterotoxin type A antibody with pH 5.7 phosphate buffer to a concentration of 0.01mg / ml Dilute staphylococcal enterotoxin type B antibody to a concentration of 0.05mg / ml Antibody, make the concentration 0.05mg / ml, dilute papaverine antibody to make the concentration 0.5mg / ml;

[0037] ② Add the four kinds of antibody dilutions to different areas on the surface of the glass slide with a spotting instrument, place at 36°C and saturated humidity for 1 hour, rinse with pH 5.7 phosphate buffer, then rinse with distilled water, and dry;

[0038] ③ Add 20 μl of calf serum with a volume percentage of 24% to the area on the glass slide containing staphylococcal enterotoxin type A antibody, staphylococcal enterotoxin type B antibody, and staphylococcal enterotoxin type C antibody for blocking. The region containing the papaverine antibody on the glass ...

Embodiment 3

[0040] Step 1 and step 2 are the same as embodiment 1.

[0041] 3. Antibody fixation, which includes:

[0042] ①Dilute Staphylococcal enterotoxin type A antibody with pH 8.0 phosphate buffer to a concentration of 2.0mg / ml Dilute staphylococcal enterotoxin type B antibody to a concentration of 2.0mg / ml Dilute staphylococcal enterotoxin type C Antibody, make the concentration 1.0mg / ml, dilute the papaverine antibody to make the concentration 2mg / ml;

[0043] ② Add the four kinds of antibody dilutions to different areas on the surface of the glass slide with a spotting instrument, place at 38°C and saturated humidity for 4 hours, rinse with pH 8.0 phosphate buffer, then rinse with distilled water, and dry;

[0044]③ Add 20 μl of calf serum with a volume percentage of 28% to the area on the glass slide containing staphylococcal enterotoxin type A antibody, staphylococcal enterotoxin type B antibody, and staphylococcal enterotoxin type C antibody for blocking. The region containi...

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Abstract

The present invention discloses an immune chip for detecting staphylococcal enterotoxin and papaverina dn its preparation process. The immune chip has slide glass with surface treated with silane as aldehydation reagent and crosslinked with glutaraldehyde as double-function crosslinking reagent, and connected via covalent bond with the molecule of at least one kind of antibody staphylococcal enteriotoxin A antibody, staphylococcal enterotoxin B antibody, staphylococcal enterotoxin C antibody and papaverine antibody. The detection with the immune chip is simple, effective and low in cost.

Description

technical field [0001] The invention relates to an immune chip and a preparation method thereof, in particular to an immune chip for detecting staphylococcus enterotoxin and papaverine and a preparation method thereof. Background technique [0002] Biological immune chip refers to the high-density antigen or antibody microarray coated on the solid phase carrier, which is a new type of biological chip after the gene chip. The immune chip is composed of antigen or antibody microarrays immobilized on different types of support media. The position and composition of the immobilized molecules in the array are known. The antigen reacts with the recognition molecules on the chip, and then is detected by a specific scanning device, and the results are analyzed and processed by a computer. Compared with traditional techniques for detecting contaminants in food, immunochips have many advantages. Traditional detection methods can only detect one pollu...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/531G01N33/543G01N33/547G01N33/569
Inventor 高志贤
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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