Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Indirect competitive ELISA (Enzyme Linked Immunosorbent Assay) method based on multi-epitope tandem peptides and used for synchronously detecting staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin G (SEG)

A technology for simultaneous detection and tandem peptides, applied in the field of immunoassays, can solve the problems of unsatisfactory detection specificity and sensitivity, blind spots in the detection of new enterotoxins, limited detection range, etc., to reduce subjectivity and reagent storage time. Long and simple results

Active Publication Date: 2013-11-27
ANHUI AGRICULTURAL UNIVERSITY
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-quality ELISA kits have been developed abroad for the detection of SE in foods such as milk and meat products, but they are expensive
In 2010, my country also designated the indirect ELISA method as the national standard method for detecting classic SE, but the detection range is limited, and only classic enterotoxins can be detected. There are blind spots in the detection of new enterotoxins, and the detection specificity and sensitivity are not satisfactory. false positive
At present, the relevant SE antigen detection methods declared in China can only detect classical enterotoxin, and the quality of detection reagents needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Indirect competitive ELISA (Enzyme Linked Immunosorbent Assay) method based on multi-epitope tandem peptides and used for synchronously detecting staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin G (SEG)
  • Indirect competitive ELISA (Enzyme Linked Immunosorbent Assay) method based on multi-epitope tandem peptides and used for synchronously detecting staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin G (SEG)
  • Indirect competitive ELISA (Enzyme Linked Immunosorbent Assay) method based on multi-epitope tandem peptides and used for synchronously detecting staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin G (SEG)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0048] The following examples further illustrate the present invention, but do not limit the present invention.

[0049] The experimental methods in the following examples are conventional methods unless otherwise specified.

[0050] The percentages in the following examples, unless otherwise specified, are all percentages by mass.

[0051] The proportions in the following examples are all volume proportions unless otherwise specified.

[0052] 1. Preparation of multi-epitope tandem peptide SE A / G of Staphylococcus aureus type A and type G enterotoxin

[0053] 1.1 Design and gene synthesis of multi-epitope tandem peptide SE A / G

[0054] Design the SE A / G gene of the S. aureus type A and G type enterotoxin multi-epitope tandem peptide SE A / G, that is, use the AAY (alanine-alanine-tyrosine) nucleotide sequence (SEQ ID NO: 8) to convert the SE The nucleotide sequences of the 4 B-cell linear epitope regions of A and the 3 dominant B-cell linear epitopes of SE G are concatenated into the mul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of immunoassay and particularly relates to an indirect competitive ELISA (Enzyme Linked Immunosorbent Assay) method based on multi-epitope tandem peptides and used for synchronously detecting staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin G (SEG). The method mainly comprises preparation of SEA and SEG multi-epitope tandem peptide peptides (including antigens), preparation of anti-rabbit SEA and SEG antibodies, sample pretreatment and establishment of the indirect competitive ELISA detection method based on multi-epitope tandem peptides. The indirect competitive ELISA method comprises the following steps: (1) enveloping; (2) plate washing; (3) sealing; (4) plate washing; (5) adding standard substances, samples to be detected and specific antibodies in sequence; (6) plate washing; (7) adding ELIAS secondary antibodies; (8) plate washing; (9) developing and ending reaction. The method provided by the invention has the characteristics of high specificity, high sensitivity and good repeatability, is suitable for quick, synchronous and quantificational detection of SEA and SEG in foods such as milk and dairy products.

Description

Technical field [0001] The invention belongs to the technical field of immunoassays, and relates to a rapid, specific, sensitive and simultaneous detection method of Staphylococcus aureus type A and G enterotoxin in foods such as milk and dairy products, and specifically is an indirect competition based on multi-epitope tandem peptides ELISA method. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus, referred to as Staphylococcus aureus) is an important food-borne pathogen, which is widely present in air, water, dust, and human and animal excrement. Staphylococcus aureus is easy to grow and reproduce and secrete enterotoxin (Staphylococcal Enterotoxin, SE) in foods with high water content, protein and starch, especially milk and dairy products. Since SE has strong thermal stability and toxicity, food contaminated with Staphylococcus aureus can be killed after heat treatment, but the SE produced by it is still pathogenic, and only a small amount of SE can ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
Inventor 李槿年梁明燕刘雪兰张婷婷李冠青
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com