3 results about "Enterotoxin gene" patented technology

The application belongs to the field of microbiology and veterinary microbiological technology, and relates to a strain of enterotoxigenic Escherichia coli carrying F5 and F41 fimbriae and application thereof. The strain is isolated from a fecal sample of a diarrheal calf, and is confirmed to be enterotoxigenic Escherichia coli carrying F5 fimbriae-related genes, F41 fimbriae-related genes and ST enterotoxin estA gene through isolation and culture, morphological identification, biochemical identification, fimbriae gene and virulence gene PCR detection and whole genome sequencing analysis. The strain has been preserved in the China General Microbiological Culture Collection Center on June 13, 2022, and the preservation number is CGMCC No. 25077. Pathogenicity tests show that the strain has strong virulence and can stably establish an animal infection model. Drug sensitivity tests show that the strain has a specific sensitivity spectrum to a variety of antibacterial drugs. The strain can be used for animal infection model establishment, antibacterial drug evaluation, calf diarrhea pathogenesis research, prevention and control technology research, and can also be used for preparing biological agents for preventing or diagnosing calf diarrhea.

The present invention relates to a double mutant heat labile enterotoxin gene comprising a spacer and, more specifically, to a use of a heat labile enterotoxin protein, encoded by the gene, for enhancing immunity. It was found that when the double mutant heat labile enterotoxin gene comprising a spacer according to the present invention is introduced to host cells, the translations of LTA and LTB constituting the labile enterotoxin gene do not affect each other, and thus LTB expression increases more than fivefold. In addition, it was experimentally confirmed that the expressed double mutant heat labile enterotoxin protein exhibits weak enterotoxic activity and simultaneously activates humoral and cellular immunity. Thus, the double mutant heat labile enterotoxin gene according to the present invention can be variously utilized in the fields of vaccines and vaccine adjuvants.

This invention discloses a primer-probe composition for detecting Staphylococcus aureus and enterotoxins based on LAMP technology and its application, belonging to the field of molecular diagnostic technology. The primer-probe composition provided by this invention can specifically recognize Staphylococcus aureus and simultaneously identify the enterotoxin genes entA, entB, entC, entD, and entE of Staphylococcus aureus. Based on this, a highly sensitive and specific detection kit can be formed, which can simultaneously perform multiple LAMP reactions in a single tube. It has the advantages of low cost, convenient operation, short detection time, and simple result interpretation, meeting the needs of grassroots testing departments for speed and convenience, and facilitating real-time on-site testing within the production line for enterprises.