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Applications of Bacteroides fragilis in treatment and/or prevention of obesity or diabetes mellitus

A technology of Bacteroides fragilis and obesity, applied in the field of application of Bacteroides fragilis in the treatment and/or prevention of obesity or diabetes, to achieve the effect of no drug resistance and broad application prospects

Active Publication Date: 2017-02-15
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of B. fragilis to treat obesity and diabetes and its complications has not been documented so far

Method used

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  • Applications of Bacteroides fragilis in treatment and/or prevention of obesity or diabetes mellitus
  • Applications of Bacteroides fragilis in treatment and/or prevention of obesity or diabetes mellitus
  • Applications of Bacteroides fragilis in treatment and/or prevention of obesity or diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Isolation and Purification of Bacteroides fragilis ZY-312

[0049] Reagents and Instruments

[0050] (1) Medium A: Bacteroides-bile-escin (BBE) agar (Qingdao Haibo Biotechnology Co., Ltd., product number: HB7028) was added with an improved formula, and the specific components are shown in Table 1:

[0051] Table 1 Culture and composition

[0052]

[0053] (2) Medium B: Bacteroides-bile-escin (BBE) agar (Qingdao Haibo Biotechnology Co., Ltd., product number: HB7028) was added with an improved formula, and the specific ingredients are shown in Table 2:

[0054] Table 2 Culture and composition

[0055]

[0056]

[0057] (3) Medium C: Fetal bovine serum was added to Brinell Broth (Qingdao Haibo Biotechnology Co., Ltd., product number: HB0241), and the addition amount was 5% (v / v) (Zhejiang Tianhang Biotechnology Co., Ltd., Brand: Sijiqing, Item No.: HB0205).

[0058] (4) Experimental equipment

[0059] 2.5L sealed culture tank (Mitsubishi Gas Chemical Co., Ltd...

Embodiment 2

[0097] Identification of Bacteroides fragilis ZY-312

[0098] The polymerase chain reaction (PCR) primer (synthesized by Yingwei Jieji (Shanghai) Trading Co., Ltd.) sequence is as follows:

[0099] Primer pair 1:

[0100] Forward primer: 5'-ACGCTTGCACCCCTCCGTATTA-3'

[0101] Reverse primer: 5'-GCTTAGAGTTTGATCCTGGCTCAG-3'

[0102] Primer pair 2:

[0103] Forward primer: 5'-TGGGTGGTTGCTGCCTGGACACA-3'

[0104] Reverse primer: 5'-CATCCGGGTATGGATATGAA-3'

[0105] Primer pair 3:

[0106] Forward primer: 5'-GATGCTCCAGTTACAGCTTCCATTG-3'

[0107] Reverse primer: 5'-CGCCCAGTATATGACCTAGTTCGTG-3'

[0108] bft gene primer pair:

[0109] Forward primer: 5'-GACGGTGTATGTGATTTGTCTGAGAGA-3'

[0110] Reverse primer: 5'-ATCCCTAAGATTTATTATCCCAAGTA-3'

[0111] 1. PCR identification (PCR is the polymerase chain reaction, which is a commonly used method for rapidly amplifying genes)

[0112] (1) 16S rRNA sequence determination

[0113] The above strains were inoculated on medium A, and cu...

Embodiment 3

[0133] Tolerance of Bacteroides fragilis ZY-312 to Gastric Acid

[0134] 1. Artificial gastric juice preparation (according to the 2010 "Chinese Pharmacopoeia" artificial gastric juice preparation method)

[0135] Dissolve 23.4mL of concentrated HCl in 100mL of purified water to obtain dilute hydrochloric acid. Take 8.2mL dilute hydrochloric acid, add 400mL purified water and 5g pepsin ( Porcine source, 1:15000), dilute to 500mL. Stir overnight at 37°C with magnetic force to obtain artificial gastric juice.

[0136] 2. Cell preparation

[0137] Collect the bacterial solution, centrifuge, discard the supernatant, resuspend in normal saline, centrifuge again, discard the supernatant, and keep the bacteria for later use.

[0138] 3. Add artificial gastric juice to determine the number of viable bacteria

[0139] Add artificial gastric juice to the bacteria, resuspend, and measure the number of viable bacteria at 0, 1, 2, and 3 hours respectively.

[0140] The 10-fold seria...

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PUM

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Abstract

The invention relates to applications of Bacteroides fragilis in treatment and / or prevention of obesity or diabetes mellitus, particularly to applications of Bacteroides fragilis in preparation of pharmaceutical compositions, foods, health products and food additives for treatment and / or prevention of obesity or diabetes mellitus, wherein the Bacteroides fragilis is ZY-312, is preserved in the China general microbiological culture collection center on April 2, 2015, and has the preservation number of CGMCC No.10685. According to the present invention, the Bacteroides fragilis provides good effects for treatment and / or prevention of obesity or diabetes mellitus, has characteristics of no drug resistance generation, safety, no toxicity, no enterotoxin gene bft and broad application prospects, and further has beneficial characteristics of cholate resistance, gastric acid resistance and the like compared with the existing strains.

Description

technical field [0001] The invention relates to the technical fields of microorganisms, medicines, health care products, food and daily chemical products, in particular to the application of Bacteroides fragilis in the treatment and / or prevention of obesity or diabetes. Background technique [0002] Diabetes mellitus (Diabetes Mellitus) is a metabolic disease with multiple etiologies. It is a disease caused by widespread metabolic disorders caused by defects in insulin secretion or action. Diabetes is a disease characterized by persistent chronic hyperglycemia, which can also cause metabolic disorders such as protein, fat, water, and electrolytes in the body. [0003] The etiology of diabetes is currently divided into two aspects: genetic factors and environmental factors. Genetic factors: Significant genetic heterogeneity exists in either type 1 or type 2 diabetes. Diabetes tends to occur in families, and 1 / 4 to 1 / 2 of patients have a family history of diabetes. Clinical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P3/10A61P3/04
CPCA61K35/741
Inventor 智发朝白杨王晔刘洋洋王从峰
Owner GUANGZHOU ZHIYI PHARMA INC
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