Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Stn gene oligonucleotide primers for detecting salmonella species and detection process using the same

An oligonucleotide and Salmonella technology, which is applied to stn gene oligonucleotide primers for detecting Salmonella species and the detection field using the primers, and can solve problems such as time-consuming detection of Salmonella

Inactive Publication Date: 2006-05-10
科学和工业研究委员会
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Detection of Salmonella by conventional bacteriological methods is very time consuming, usually at least 5 days

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Stn gene oligonucleotide primers for detecting salmonella species and detection process using the same
  • Stn gene oligonucleotide primers for detecting salmonella species and detection process using the same
  • Stn gene oligonucleotide primers for detecting salmonella species and detection process using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] Correspondingly, the present invention relates to oligonucleotide primers SEQ ID No.1 to SEQ ID No.21 specific to the Salmonella enterotoxin gene (stn) gene, and these primers are useful for rapid and specific screening of Salmonella; The present invention also relates to a rapid and specific detection method for the Salmonella enterotoxin gene (stn) gene, the method uses oligonucleotide primers SEQ ID No.1 to SEQ ID No.21 for polymerase chain reaction (PCR), To determine whether the parasite Salmonella exists in the subject, the method comprises the following steps: preparing a DNA template of the gene, using the primers to amplify the template by PCR, electrophoresis of the PCR product on the gel, and detecting the parasite.

[0013] In one embodiment of the present invention, wherein, the oligonucleotide primers SEQ ID No.1~SEQ ID No.21 are specific to the Salmonella enterotoxin gene (stn) gene, and are useful for rapid and specific screening of Salmonella .

[001...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to oligonucleotide primers having SEQ ID NOs. 1 to 21 specific for Salmonella enterotoxin gene (stn) gene, useful for rapid and specific screening of Salmonella. The present invention relates to a process for the rapid and specific detection of Salmonella enterotoxin gene (stn) gene in a subject for the presence of parasite Salmonella using oligonucleotide primers having SEQ ID NOs. 1 to 21 for Polymerase Chain Reaction (PCR), said process comprising the steps of preparing a DNA template, amplifying the template using the primers by PCR, running the PCR products on gel, and detecting the Salmonella.

Description

technical field [0001] The present invention relates to the oligonucleotide primers SEQ ID No.1~SEQ ID No.21 specific to Salmonella (Salmonella) enterotoxin gene (stn), these primers are useful for rapid and specific screening of Salmonella; The invention also relates to a rapid and specific detection method for Salmonella enterotoxin gene (stn), which uses oligonucleotide primers SEQ ID No.1 to SEQ ID No.21 for polymerase chain reaction (PCR) to determine the Whether biological Salmonella exists in the test object, the method comprises the following steps: preparing the DNA template of the gene, using the primers to amplify the template by PCR, performing electrophoresis on the PCR product on the gel, and analyzing the detection of parasites. Background technique [0002] Salmonella species are facultative intracellular parasites that invade the mucous membranes of epithelial cells and are transmitted to humans mainly through water, meat, eggs and poultry products. Salmon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/88
CPCY02A50/30
Inventor 吴拉姆·纳比·卡齐维杰施瓦·维尔马赛义德·里亚齐-乌-哈桑
Owner 科学和工业研究委员会
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com