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Use of Bacteroides fragilis in the treatment and/or prevention of obesity or diabetes

A technology of Bacteroides fragilis and obesity, applied in the fields of microorganisms, medicines and daily chemical products, to achieve broad application prospects without the effect of drug resistance

Active Publication Date: 2019-12-24
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of B. fragilis to treat obesity and diabetes and its complications has not been documented so far

Method used

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  • Use of Bacteroides fragilis in the treatment and/or prevention of obesity or diabetes
  • Use of Bacteroides fragilis in the treatment and/or prevention of obesity or diabetes
  • Use of Bacteroides fragilis in the treatment and/or prevention of obesity or diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Isolation and Purification of Bacteroides fragilis ZY-312

[0045] Reagents and Instruments

[0046] (1) Medium A: Bacteroides-bile-escin (BBE) agar (Qingdao Haibo Biotechnology Co., Ltd., product number: HB7028) was added with an improved formula, and the specific components are shown in Table 1:

[0047] Table 1 Culture and composition

[0048]

[0049]

[0050] (2) Medium B: Bacteroides-bile-escin (BBE) agar (Qingdao Haibo Biotechnology Co., Ltd., product number: HB7028) was added with an improved formula, and the specific ingredients are shown in Table 2:

[0051] Table 2 culture and composition

[0052]

[0053] (3) Medium C: Fetal bovine serum was added to Brinell Broth (Qingdao Haibo Biotechnology Co., Ltd., product number: HB0241), and the addition amount was 5% (v / v) (Zhejiang Tianhang Biotechnology Co., Ltd., Brand: Sijiqing, Item No.: HB0205).

[0054] (4) Experimental equipment

[0055] 2.5L sealed culture tank (Mitsubishi Gas Chemical Co., Ltd...

Embodiment 2

[0094] Identification of Bacteroides fragilis ZY-312

[0095] The polymerase chain reaction (PCR) primer (synthesized by Yingwei Jieji (Shanghai) Trading Co., Ltd.) sequence is as follows:

[0096] Primer pair 1:

[0097] Forward primer: 5'-ACGCTTGCACCCCTCCGTATTA-3'

[0098] Reverse primer: 5'-GCTTAGAGTTTGATCCTGGCTCAG-3'

[0099] Primer pair 2:

[0100] Forward primer: 5'-TGGGTGGTTGCTGCCTGGACACA-3'

[0101] Reverse primer: 5'-CATCCGGGTATGGATATGAA-3'

[0102] Primer pair 3:

[0103] Forward primer: 5'-GATGCTCCAGTTACAGCTTCCATTG-3'

[0104] Reverse primer: 5'-CGCCCAGTATATGACCTAGTTCGTG-3'

[0105] bft gene primer pair:

[0106] Forward primer: 5'-GACGGTGTATGTGATTTGTCTGAGAGA-3'

[0107] Reverse primer: 5'-ATCCCTAAGATTTATTATCCCAAGTA-3'

[0108] 1. PCR identification (PCR is the polymerase chain reaction, which is a commonly used method for rapidly amplifying genes)

[0109] (1) 16S rRNA sequence determination

[0110] The above strains were inoculated on medium A, and cu...

Embodiment 3

[0130] Tolerance of Bacteroides fragilis ZY-312 to Gastric Acid

[0131] 1. Artificial gastric juice preparation (according to the 2010 "Chinese Pharmacopoeia" artificial gastric juice preparation method)

[0132] Dissolve 23.4mL of concentrated HCl in 100mL of purified water to obtain dilute hydrochloric acid. Take 8.2mL dilute hydrochloric acid, add 400mL purified water and 5g pepsin ( Porcine source, 1:15000), dilute to 500mL. Stir overnight at 37°C with magnetic force to obtain artificial gastric juice.

[0133] 2. Cell preparation

[0134] Collect the bacterial solution, centrifuge, discard the supernatant, resuspend in normal saline, centrifuge again, discard the supernatant, and keep the bacteria for later use.

[0135] 3. Add artificial gastric juice to determine the number of viable bacteria

[0136] Add artificial gastric juice to the bacteria, resuspend, and measure the number of viable bacteria at 0, 1, 2, and 3 hours respectively.

[0137] The 10-fold serial ...

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PUM

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Abstract

Provided is an application of bacteroides fragilis in treating and / or preventing obesity or diabetes, and also provided is an application of the bacteroides fragilis in preparing pharmaceutical compositions, food, healthcare products, and food additives for treating and / or preventing obesity or diabetes. The bacteroides fragilis is bacteroides fragilis ZY-312.

Description

technical field [0001] The invention relates to the technical fields of microorganisms, medicines and daily chemical products, in particular to the application of Bacteroides fragilis in the treatment and / or prevention of obesity or diabetes. Background technique [0002] Diabetes mellitus (Diabetes Mellitus) is a metabolic disease with multiple etiologies. It is a disease caused by widespread metabolic disorders caused by defects in insulin secretion or action. Diabetes is a disease characterized by persistent chronic hyperglycemia, which can also cause metabolic disorders such as protein, fat, water, and electrolytes in the body. [0003] The etiology of diabetes is currently divided into two aspects: genetic factors and environmental factors. Genetic factors: Significant genetic heterogeneity exists in either type 1 or type 2 diabetes. Diabetes tends to occur in families, and 1 / 4 to 1 / 2 of patients have a family history of diabetes. Clinically, there are at least 60 ge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/741A61P3/10A61P3/04
CPCA61K35/741
Inventor 智发朝白杨王晔刘洋洋王从峰
Owner GUANGZHOU ZHIYI PHARMA INC
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