2 results about "Staphylococcus aureus enterotoxin" patented technology

The invention belongs to the technical field of biosensors, and discloses a photoelectrochemical immunosensor based on a dendritic peptide tetramer as well as a preparation method and application of the photoelectrochemical immunosensor. The dendritic peptide tetramer is adopted to construct the photoelectrochemical immunosensor for the first time, and the method comprises the following steps: preparing an HsGDY / Cu2O photocathode, and sequentially anchoring an antibody probe and the dendritic peptide tetramer on the surface of the photocathode. Through specific binding of a target object and the antibody probe, a significant steric hindrance effect is generated on an electrode interface, interface charge transfer is hindered, photocurrent signal change is caused, and detection of the target object staphylococcus aureus enterotoxin A is realized. The photoelectrochemical immunosensor provided by the invention is simple, convenient and efficient in preparation process, has excellent food matrix biological pollution resistance, can effectively inhibit non-specific adsorption so as to significantly improve the sensitivity, specificity and accuracy of detection, is suitable for the field of food safety detection, and is suitable for market popularization and application.

This invention discloses a biosensor and electrochemical detection method for detecting Staphylococcus aureus enterotoxin A (SEA). The biosensor includes an electrode modified with an MXene / AuNPs nanocomposite material, a recognition probe complex, a signal probe complex, and a capture probe. This invention increases the electrode's conductivity and specific surface area by modifying the electrode surface with the MXene / AuNPs complex. Simultaneously, it introduces a chain substitution reaction signal amplification strategy, achieving efficient amplification of the electrochemical signal and significantly improving detection sensitivity. This sensor combines the excellent properties of nanomaterials with a nucleic acid signal amplification strategy, exhibiting good specificity, stability, and repeatability in SEA detection. Furthermore, this sensor requires no complex pretreatment, has a rapid response, and shows great potential for detection in practical food samples.