Applications of Bacteroides fragilis in prevention and/or treatment of meningitis

A technology of Bacteroides fragilis and meningitis, applied in the fields of microorganisms, health care products, food and daily chemical products, and pharmaceuticals, to achieve the effect of no drug resistance and broad application prospects

Active Publication Date: 2017-02-15
石家庄普维生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of B. fragilis to treat me...

Method used

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  • Applications of Bacteroides fragilis in prevention and/or treatment of meningitis
  • Applications of Bacteroides fragilis in prevention and/or treatment of meningitis
  • Applications of Bacteroides fragilis in prevention and/or treatment of meningitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Isolation and Purification of Bacteroides fragilis ZY-312

[0048] Reagents and Instruments

[0049] (1) Medium A: Bacteroides-bile-escin (BBE) agar (Qingdao Haibo Biotechnology Co., Ltd., product number: HB7028) was added with an improved formula, and the specific components are shown in Table 1:

[0050] Table I

[0051]

[0052]

[0053] (2) Medium B: Bacteroides-bile-escin (BBE) agar (Qingdao Haibo Biotechnology Co., Ltd., product number: HB7028) was added with an improved formula, and the specific ingredients are as follows in Table 2:

[0054] Table II

[0055]

[0056] (3) Medium C: Fetal bovine serum was added to Brinell Broth (Qingdao Haibo Biotechnology Co., Ltd., product number: HB0241), and the addition amount was 5% (v / v) (Zhejiang Tianhang Biotechnology Co., Ltd., Brand: Sijiqing, Item No.: HB0205).

[0057] (4) Experimental equipment

[0058] 2.5L sealed culture tank (Mitsubishi Gas Chemical Co., Ltd., C-31)

[0059] Constant temperature in...

Embodiment 2

[0097] Identification of Bacteroides fragilis ZY-312

[0098] The polymerase chain reaction (PCR) primer (synthesized by Yingwei Jieji (Shanghai) Trading Co., Ltd.) sequence is as follows:

[0099] Primer pair 1:

[0100] Forward primer: 5'-ACGCTTGCACCCCTCCGTATTA-3'

[0101] Reverse primer: 5'-GCTTAGAGTTTGATCCTGGCTCAG-3'

[0102] Primer pair 2:

[0103] Forward primer: 5'-TGGGTGGTTGCTGCCTGGACACA-3'

[0104] Reverse primer: 5'-CATCCGGGTATGGATATGAA-3'

[0105] Primer pair 3:

[0106] Forward primer: 5'-GATGCTCCAGTTACAGCTTCCATTG-3'

[0107] Reverse primer: 5'-CGCCCAGTATATGACCTAGTTCGTG-3'

[0108] bft gene primer pair:

[0109] Forward primer: 5'-GACGGTGTATGTGATTTGTCTGAGAGA-3'

[0110] Reverse primer: 5'-ATCCCTAAGATTTATTATCCCAAGTA-3'

[0111] 1. PCR identification (PCR is the polymerase chain reaction, which is a commonly used method for rapidly amplifying genes)

[0112] (1) 16S rRNA sequence determination

[0113] The above strains were inoculated on medium A, and cu...

Embodiment 3

[0134] Tolerance of Bacteroides fragilis ZY-312 to Gastric Acid

[0135] 1. Artificial gastric juice preparation (according to the 2010 "Chinese Pharmacopoeia" artificial gastric juice preparation method)

[0136] Dissolve 23.4mL of concentrated HCl in 100mL of purified water to obtain dilute hydrochloric acid. Take 8.2mL dilute hydrochloric acid, add 400mL purified water and 5g pepsin ( Porcine source, 1:15000), dilute to 500mL. Stir overnight at 37°C with magnetic force to obtain artificial gastric juice.

[0137] 2. Cell preparation

[0138] Collect the bacterial solution, centrifuge, discard the supernatant, resuspend in normal saline, centrifuge again, discard the supernatant, and keep the bacteria for later use.

[0139] 3. Add artificial gastric juice to determine the number of viable bacteria

[0140] Add artificial gastric juice to the bacteria, resuspend, and measure the number of viable bacteria at 0, 1, 2, and 3 hours respectively.

[0141] The 10-fold seria...

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PUM

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Abstract

The invention relates to applications of Bacteroides fragilis in prevention and/or treatment of meningitis, particularly to applications of Bacteroides fragilis in preparation of drugs, pharmaceutical compositions, foods, health products and food additives for prevention and/or treatment of meningitis, wherein the Bacteroides fragilis is ZY-312, is preserved in the China general microbiological culture collection center on April 2, 2015, and has the preservation number of CGMCC No.10685. According to the present invention, the Bacteroides fragilis provides good effects for prevention and/or treatment of meningitis, has characteristics of no drug resistance generation, safety, no toxicity, no enterotoxin gene bft and broad application prospects, and further has beneficial characteristics of cholate resistance, gastric acid resistance and the like compared with the existing strains.

Description

technical field [0001] The invention relates to the technical fields of microorganisms, medicines, health care products, food and daily chemical products, in particular to the application of Bacteroides fragilis in the prevention and / or treatment of meningitis. Background technique [0002] Bacterial meningitis is a serious infectious disease of the central nervous system, common in adults, but the most common in neonates. Neonates are high-risk groups for bacterial meningitis because of their low immune function, imperfect leukocyte chemotaxis, adhesion and migration, complement activity, and low concentrations of specific and non-specific antibodies in the body. [0003] In the 1930s, the advent of sulfa drugs reduced the fatality rate of spherical meningitis to 5%-15%. By 1944, 87 children with Haemophilus influenzae type b meningitis were treated with sulfonamides and intravenous rabbit antiserum, reducing the fatality rate to 22%. In the early 1960s, chloramphenicol (...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P25/00A61P31/04A23L33/00A23L33/10C12N1/20C12R1/01
CPCA61K35/74C12N1/20C12N1/205C12R2001/01
Inventor 智发朝白杨王晔刘洋洋王从峰
Owner 石家庄普维生物科技有限公司
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