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Application of Bacteroides fragilis in Animal Breeding

A technology of Bacteroides fragilis and animal breeding, which is applied in the fields of health care products, food and daily chemical products, microbes, and pharmaceuticals. live effect

Active Publication Date: 2020-11-06
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is still a lack of microecological preparations containing Bacteroides fragilis on the market, and the application field of this bacteria needs to be expanded

Method used

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  • Application of Bacteroides fragilis in Animal Breeding
  • Application of Bacteroides fragilis in Animal Breeding
  • Application of Bacteroides fragilis in Animal Breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Isolation and purification of Bacteroides fragilis ZY-312

[0040] Reagents and Instruments

[0041] (1) Medium A: Bacteroides-Bile-Escin (BBE) agar (Qingdao Haibo Biotechnology Co., Ltd., product number: HB7028) was added with an improved formula, and the specific components are shown in Table 1:

[0042] Table I

[0043]

[0044] (2) Medium B: Bacteroides-bile-escin (BBE) agar (Qingdao Haibo Biotechnology Co., Ltd., product number: HB7028) was added with an improved formula, and the specific ingredients are as follows in Table 2:

[0045] Table II

[0046]

[0047]

[0048] (3) Medium C: Fetal bovine serum was added to Brinell Broth (Qingdao Haibo Biotechnology Co., Ltd., product number: HB0241), and the addition amount was 5% (v / v) (Zhejiang Tianhang Biotechnology Co., Ltd., Brand: Sijiqing, Item No.: HB0205).

[0049] (4) Experimental equipment

[0050] 2.5L sealed culture tank (Mitsubishi Gas Chemical Co., Ltd., C-31)

[0051] Constant temperature in...

Embodiment 2

[0088] Identification of Bacteroides fragilis ZY-312

[0089] The polymerase chain reaction (PCR) primer (synthesized by Yingwei Jieji (Shanghai) Trading Co., Ltd.) sequence is as follows:

[0090] Primer pair 1:

[0091] Forward primer: 5'-ACGCTTGCACCCCTCCGTATTA-3'

[0092] Reverse primer: 5'-GCTTAGAGTTTGATCCTGGCTCAG-3'

[0093] Primer pair 2:

[0094] Forward primer: 5'-TGGGTGGTTGCTGCCTGGACACA-3'

[0095] Reverse primer: 5'-CATCCGGGTATGGATATGAA-3'

[0096] Primer pair 3:

[0097] Forward primer: 5'-GATGCTCCAGTTACAGCTTCCATTG-3'

[0098] Reverse primer: 5'-CGCCCAGTATATGACCTAGTTCGTG-3'

[0099] bft gene primer pair:

[0100] Forward primer: 5'-GACGGTGTATGTGATTTGTCTGAGAGA-3'

[0101] Reverse primer: 5'-ATCCCTAAGATTTATTATCCCAAGTA-3'

[0102] 1. PCR identification (PCR is the polymerase chain reaction, which is a commonly used method for rapidly amplifying genes)

[0103] (1) 16S rRNA sequence determination

[0104] The above strains were inoculated on medium A, and cult...

Embodiment 3

[0125] Tolerance of Bacteroides fragilis ZY-312 to Gastric Acid

[0126] 1. Artificial gastric juice preparation (according to the 2010 "Chinese Pharmacopoeia" artificial gastric juice preparation method)

[0127] Dissolve 23.4mL of concentrated HCl in 100mL of purified water to obtain dilute hydrochloric acid. Take 8.2mL dilute hydrochloric acid, add 400mL purified water and 5g pepsin ( Porcine source, 1:15000), dilute to 500mL. Stir overnight at 37°C with magnetic force to obtain artificial gastric juice.

[0128] 2. Cell preparation

[0129] Collect the bacterial solution, centrifuge, discard the supernatant, resuspend in normal saline, centrifuge again, discard the supernatant, and keep the bacteria for later use.

[0130] 3. Add artificial gastric juice to determine the number of viable bacteria

[0131] Add artificial gastric juice to the bacteria, resuspend, and measure the number of viable bacteria at 0, 1, 2, and 3 hours respectively.

[0132] The 10-fold seria...

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Abstract

The application of Bacteroides fragilis in animal breeding, specifically relates to the application of Bacteroides fragilis in the prevention and / or treatment of animal gastrointestinal diseases and its application in pharmaceutical compositions and feed additives, wherein Bacteroides fragilis is ZY‑312 , the deposit number is CGMCC No.10685. The Bacteroides fragilis ZY‑312 of the present invention does not contain the enterotoxin gene bft, has significantly higher tolerance to bile salts and gastric acid than the existing Bacteroides fragilis, and can effectively prevent and / or treat gastrointestinal diseases, improve animal immunity, disease resistance, reduce animal morbidity, increase animal feed conversion rate, increase animal weight, promote its growth and production performance, reduce the use of antibiotics, do not produce drug resistance, and are safe and non-toxic Toxins can improve the quality of meat, eggs, milk and other products, and can also eliminate antibiotic drug residues, purify the stable environment, and protect the ecological environment.

Description

technical field [0001] The invention relates to the technical fields of microorganisms, medicines, health care products, food and daily chemical products, in particular to the application of Bacteroides fragilis in animal breeding. Background technique [0002] my country is a big animal husbandry country. According to the statistics of relevant departments, the output of pigs, poultry, meat and eggs in my country ranks first in the world. Poor ability to be sick. According to the recent national livestock disease census, there are hundreds of various types of livestock and poultry diseases in my country. Since the 1970s, 37 new livestock and poultry diseases have been added. Livestock and poultry diseases have become a major obstacle to the development of my country's animal husbandry. Therefore, during the breeding process, farmers will illegally use growth-promoting drugs, sleeping pills, contraceptives and other feed additives, abuse antibiotics, etc. to increase their p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A61K35/741A61P1/00
CPCA23K20/00C12N1/20C12N1/205C12R2001/01
Inventor 智发朝白杨王晔刘洋洋王从峰
Owner GUANGZHOU ZHIYI PHARMA INC
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