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Stn gene oligonucleotide primers for detecting salmonella species and detection process using the same

A Salmonella and oligonucleotide technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, and other methods of inserting foreign genetic materials, etc., can solve the problem of time-consuming detection of Salmonella

Inactive Publication Date: 2011-11-09
科学和工业研究委员会
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Detection of Salmonella by conventional bacteriological methods is very time consuming, usually at least 5 days

Method used

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  • Stn gene oligonucleotide primers for detecting salmonella species and detection process using the same
  • Stn gene oligonucleotide primers for detecting salmonella species and detection process using the same
  • Stn gene oligonucleotide primers for detecting salmonella species and detection process using the same

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Embodiment Construction

[0012] Correspondingly, the present invention relates to oligonucleotide primers SEQ ID No.1 to SEQ ID No.21 specific to the Salmonella enterotoxin gene (stn) gene, and these primers are useful for rapid and specific screening of Salmonella; The present invention also relates to a rapid and specific detection method for the Salmonella enterotoxin gene (stn) gene, the method uses oligonucleotide primers SEQ ID No.1 to SEQ ID No.21 for polymerase chain reaction (PCR), To determine whether the parasite Salmonella exists in the subject, the method comprises the following steps: preparing a DNA template of the gene, using the primers to amplify the template by PCR, electrophoresis of the PCR product on the gel, and detecting the parasite.

[0013] In one embodiment of the present invention, wherein, the oligonucleotide primers SEQ ID No.1~SEQ ID No.21 are specific to the Salmonella enterotoxin gene (stn) gene, and are useful for rapid and specific screening of Salmonella .

[001...

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Abstract

The present invention relates to oligonucleotide primers having SEQ ID NO.1 to SEQ ID NO.21 specific for Salmonella enterotoxin gene (stn) gene, useful for rapid and specific screening of Salmonella. The present invention relates to a process for the rapid and specific detection of Salmonella enterotoxin gene (stn) gene in a subject for the presence of parasite Salmonella using oligonucleotide primers having SEQ ID NO.1 to SEQ ID NO.21 for Polymerase Chain Reaction (PCR), the process comprising the steps of preparing a DNA template, amplifying the template using the primers by PCR, running the PCR products on gel, and detecting the Salmonella.

Description

technical field [0001] The present invention relates to the oligonucleotide primers SEQ ID No.1~SEQ ID No.21 specific to Salmonella (Salmonella) enterotoxin gene (stn), these primers are useful for rapid and specific screening of Salmonella; The invention also relates to a rapid and specific detection method for Salmonella enterotoxin gene (stn), which uses oligonucleotide primers SEQ ID No.1 to SEQ ID No.21 for polymerase chain reaction (PCR) to determine the Whether biological Salmonella exists in the test object, the method comprises the following steps: preparing the DNA template of the gene, using the primers to amplify the template by PCR, performing electrophoresis on the PCR product on the gel, and analyzing the detection of parasites. Background technique [0002] Salmonella species are facultative intracellular parasites that invade the mucous membranes of epithelial cells and are transmitted to humans mainly through water, meat, eggs and poultry products. Salmon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/88
CPCY02A50/30
Inventor 吴拉姆·纳比·卡齐维杰施瓦·维尔马赛义德·里亚齐-乌-哈桑
Owner 科学和工业研究委员会
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