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C reactive protein saliva test paper strip and preparation method thereof

A technology of reactive protein and detection test strip, applied in the field of C-reactive protein saliva detection test strip and preparation thereof, can solve the problems of unsuitable patient's home self-test, unsuitable patient's self-test, inconvenient use and the like, and achieves easy popularization, High sensitivity and specificity

Active Publication Date: 2017-02-01
BEIJING JINHUAKE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing blood test strips have the following deficiencies during their use: 1. Sample collection will bring certain pain, which is difficult for many sample collectors to accept; 2. Professional medical personnel are needed when collecting blood samples, and It will cause wounds when collecting blood samples, which is potentially dangerous and inconvenient. It is not suitable for patients' home self-test
The disadvantage of this invention is that collecting blood samples will cause wounds, it is not suitable for self-test by patients, and it is inconvenient to use

Method used

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  • C reactive protein saliva test paper strip and preparation method thereof
  • C reactive protein saliva test paper strip and preparation method thereof
  • C reactive protein saliva test paper strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The steps of the preparation method of detection test strip of the present invention are as follows:

[0043] 1. Preparation of colloidal gold:

[0044] Accurately measure 100mL of purified water, put it into a round bottom flask, heat and stir until boiling. Add 2mL of 1% chloroauric acid solution into boiling water, continue heating and stirring. When the solution boils again, accurately measure 2 mL of 1.0% trisodium citrate solution and quickly add it to the solution, and continue to boil. Carefully observe the color of the solution. When the solution turns from yellow to purple-black, and finally becomes stable to wine red, start timing and continue heating for 10 minutes. After the temperature of the solution returns to room temperature, stop stirring, restore the volume to 100 mL with purified water, and store at 4°C in the dark for future use.

[0045] 2. Preparation of C-reactive protein monoclonal antibody gold label pad 2:

[0046] 2.1 Required raw materi...

Embodiment 2

[0067] The preparation method of detection test strip of the present invention is as follows:

[0068] 1. Preparation of colloidal gold:

[0069] Accurately measure 100mL of purified water, put it into a round bottom flask, heat and stir until boiling; add 2mL of 1% chloroauric acid solution into boiling water, continue heating and stirring; when the solution boils again, accurately measure 2mL1.0 Quickly add % trisodium citrate solution into the solution, and continue to boil; carefully observe the color of the solution, when the solution turns from yellow to purple-black, and finally becomes wine red and stable, start timing and continue heating for 10 minutes; wait until the temperature of the solution recovers After reaching room temperature, stop stirring, restore the volume to 100 mL with purified water, and store at 4°C in the dark for future use.

[0070] 2. Preparation of C-reactive protein monoclonal antibody gold label pad 2:

[0071] 2.1 Required raw materials: ...

Embodiment 3

[0092] Detection method of the present invention is as follows:

[0093] The principle of the invention is to coat the C-reactive protein monoclonal antibody and goat anti-mouse IgG on the nitrocellulose film respectively, and coat the colloidal gold-labeled C-reactive protein monoclonal antibody with strong specificity on the polyester film. During detection, if there is no C-reactive protein in the sample, the colloidal gold-labeled specific antibody combines with the membrane region antigen to form two red lines, one detection line T and one quality control line C, indicating that the test result is negative; When there is C-reactive protein and the concentration of C-reactive protein reaches 10mg / L, the C-reactive protein molecule will bind to the colloidal gold-labeled C-reactive protein antibody. When the sample surges forward, it will be coated on the nitrocellulose membrane. C-reactive protein monoclonal antibody captures to form a purple or red detection line visible ...

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Abstract

The invention belongs to the field of bio-detection and particularly relates to a C reactive protein saliva test paper strip and a preparation method thereof. The C reactive protein saliva test paper strip includes a reactive film and a gold marker pad. The reactive film is a nitrocellulose membrane, which has a detection line coated with a C reactive protein monoclonal antibody and a quality control line coated with goad-anti-mouse IgG. The gold marker pad is a polyester membrane that is coated with the C reactive protein monoclonal antibody marked by colloidal gold. The test paper strip, with human saliva as a detection sample, can effectively monitor the C reactive protein in saliva. The test paper strip has strong specificity and good repeatability, has high sensitivity, is 10 mg / L in lowest detection limit, is easy to operate and is free of special instruments and devices and professional training, has clear and distinguishable result and is easy to promote, and is suitable for on-site test.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a C-reactive protein saliva detection test strip and a preparation method thereof. Background technique [0002] C-reactive protein (C-reactive protein, CRP) is an acute phase reaction protein that can react with C polysaccharides of Streptococcus pneumoniae to form a complex, with a half-life of 19 hours; serum CRP is synthesized by the liver, interleukin 1b, 6 and Tumor necrosis factor is the most important regulator of its synthesis; CRP has a molecular weight of 105,500 and consists of five identical unglycosylated polypeptide subunits, each of which contains 187 amino acids. Covalently linked to form a ring-shaped pentamer, and there is an interchain disulfide bond. CRP can trigger the immune opsonization and phagocytosis of the invading cells, thus showing an inflammatory response. CRP is an extremely sensitive indicator of acute phase response. The concent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/532
CPCG01N33/532G01N33/54393G01N33/6803G01N2333/4737
Inventor 宋晓峰余强华张培培胡培丽潘文东
Owner BEIJING JINHUAKE BIOLOGICAL TECH
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