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Detection test strip for coxsackie virus A16 type (CA16) IgM (Immune Globulin M) antibody

A technology for testing test paper and test strips, which is applied to measuring devices, instruments, scientific instruments, etc., to achieve the effects of simple operation, easy promotion, and saving manpower and material resources

Active Publication Date: 2014-01-29
辽宁迪浩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, so far, there is no report on a test strip that can detect CA16-specific IgM antibodies conveniently and quickly.

Method used

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  • Detection test strip for coxsackie virus A16 type (CA16) IgM (Immune Globulin M) antibody
  • Detection test strip for coxsackie virus A16 type (CA16) IgM (Immune Globulin M) antibody
  • Detection test strip for coxsackie virus A16 type (CA16) IgM (Immune Globulin M) antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: CA16-specific IgM antibody detection test strip (colloidal gold method) (see Figure 1)

[0029] Coxsackie virus A16 type VP1 antigen was purchased from Beijing Anbot Gene Technology Co., Ltd.

[0030] The reaction support is a 6.5cm×0.4cm PCV plate; the absorbent pad is a 2cm×0.4cm oil filter paper; a 2.0cm×0.4cm nitrocellulose membrane is coated with anti-mouse IgG (2mg / ml) and CA16 antigen VP1 (2.3mg / ml), containing 0.4cm × 0.4cm colloidal gold-labeled anti-human IgM (labeled amount 25μg / ml) monoclonal antibody polyester film; sample pad 2.7cm × 0.4cm glass fiber; that is, composed of Coxsackie virus Type A16 (CA16) IgM antibody test strip (colloidal gold method).

Embodiment 2

[0031] Embodiment 2 detection method (see figure 2 )

[0032] 100-150 μl of the patient's whole blood, plasma or serum sample is directly dropped into the "4" of the test strip of Example 1, the sample solution runs up the membrane, and the result is interpreted within 10-15 minutes.

[0033] result:

[0034] If the detection sample contains CA16 IgM antibody, it will form a corresponding complex with the colloidal gold-labeled anti-human IgM monoclonal antibody on the test strip, and then combine with the CA16-specific antigen VP1 coated on the nitrocellulose membrane to form a complex. A red line, i.e. a red band is formed at T.

[0035] Regardless of whether the specimen contains the corresponding antibody or not, the colloidal gold-labeled anti-human IgM monoclonal antibody continues to crawl upward and forms a red precipitation line with the anti-mouse IgG coated on the membrane, that is, a red band is formed at "C". This line is a quality control line. If the colloid...

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Abstract

The invention provides a detection test strip for a coxsackie virus A16 type (CA16) IgM (Immune Globulin M) antibody. According to the detection test strip, a nitrocellulose membrane (NC membrane) is coated by a CA16 gene antigen (VP1) and an anti-mouse IgG; and by combining a tihuman IgM monoclonal antibody labeled by colloidal gold, a CA16 specificity IgM antibody in an infected human speciment is detected by applying a membrane chromatography capture method. The test strip for detection, which is disclosed by the invention, has the advantages of simpleness, convenience, quickness and fastness in operation, no need of special instrument or special training, distinct and easily-distinguished result and easiness for popularization; and the detection test strip is suitable for field detection and early-stage diagnosis and assisting function for CA16 infected diagnosis.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a Coxsackievirus A16 (CA16) IgM antibody detection test strip and an application thereof. Background technique [0002] Coxsackievirus (Coxsackievirus) is a serious pathogenic agent of human diseases. Its infection can lead to many diseases, from mild respiratory tract infection diseases to more serious myocarditis, pericarditis and some diseases of the nervous system, and even cause infant death. CoxV can be divided into A and B groups: Group A virus has 24 serotypes, and group B has 6 serotypes. All types of group B and type 9 of group A share a common group-specific antigen; there are cross-reactions among various virus serotypes in group B, but not in group A. The CoxV that infects the human body is mostly in group A, and the CoxV that causes hand, foot and mouth disease is mostly A16, A4, A5, A7, A10, B2, B5, and B13. CoxV is a single-stranded positive-stra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/532G01N33/569
Inventor 刘明
Owner 辽宁迪浩生物科技有限公司
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