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Fluorescence immunoassay method of procalcitonin in saliva

A procalcitonin and detection method technology, applied in the direction of measuring devices, biological testing, material inspection products, etc., to achieve the effect of simple operation, easy promotion, and high sensitivity

Inactive Publication Date: 2016-11-09
CHANGZHOU BIOWIN BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few products that use saliva for PCT rapid detection on the market

Method used

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  • Fluorescence immunoassay method of procalcitonin in saliva
  • Fluorescence immunoassay method of procalcitonin in saliva

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Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1: A kind of fluorescent immunoassay method of salivary procalcitonin, the specific steps are as follows:

[0047] (1), preparation of salivary procalcitonin fluorescent immunoassay kit

[0048] 1) Coupling of fluorescent dyes and antibodies

[0049] Mix the fluorescent dye rhodamine with an emission wavelength of 665nm and 1 mg / ml procalcitonin monoclonal antibody, react at room temperature for 3 hours, add 1 mol / L hydroxylamine hydrochloride to terminate the reaction, and separate and purify with a chromatographic column or chromatographic column to obtain a fluorescent dye Modified procalcitonin monoclonal antibody, fluorescent dye emission wavelength is 665nm.

[0050] 2) The construction of the test kit comprises the following steps:

[0051] a. Take fluorescent dye markers, add bovine serum albumin (content is 1%), sucrose (content is 10%) and surfactant Triton X-100 (content is 0.8%), and then evenly spray on the marking pad , sealed after drying at...

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Abstract

The invention relates to the technical field of immunoassay, and especially relates to a fluorescence immunoassay method of procalcitonin in saliva. A saliva collector, saliva, an anti-procalcitonin antibody, a fluorescence reagent and a detector are used in the invention. The saliva collector comprises one or a combination of a collecting tube, a collecting rod and a collecting scraper sheet; the anti-procalcitonin antibody is one or a combination of an anti-procalcitonin monoclonal antibody and an anti-procalcitonin polyclonal antibody; the fluorescence reagent is a liquid phase and / or solid phase material containing a fluorescent substance, and the fluorescent substance is one or a combination of organic fluorescence dyes or rare earth element fluorescence dyes; and the detector is a fluorescence detector. The method adopting human body saliva as a detection sample has the advantages of effective monitoring of procalcitonin in saliva, strong specificity, good repeatability, high sensitivity, simplicity in operation, no special devices, no professional training, clear and distinguishable result, easiness in promotion, and suitableness for onsite detection.

Description

technical field [0001] The invention relates to the technical field of immunoassay analysis, in particular to a fluorescent immunoassay method for salivary procalcitonin. Background technique [0002] Procalcitonin (PCT) is a non-hormone active glycoprotein containing 116 amino acids with a molecular weight of 13KD. Its half-life is 25-30h. Produced by thyroid-C cells under physiological conditions, the content is very small, and it can hardly be detected in the serum or cerebrospinal fluid of healthy people. According to the literature, when bacteria, fungi, parasites and other infectious diseases have systemic and central nervous system inflammatory reactions, the endotoxins of the bacteria can promote thyroid-C cells, liver macrophages and monocytes, lung and intestinal Tissues other than the thyroid, such as lymphocytes and neuroendocrine cells in the tract tissue, produce a large amount of PCT, which leads to an increase or persistent increase in its serum and cerebro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/557
CPCG01N33/6803G01N33/557G01N33/582
Inventor 李亚星刘凤鸣刘冰
Owner CHANGZHOU BIOWIN BIOPHARM
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