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Colloidal gold test strip for detecting hepatitis E virus IgG antibody and preparation method of colloidal gold test strip

A technology of hepatitis E virus and colloidal gold test paper, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of complicated operation, high cost, long time consumption, etc. Effect

Inactive Publication Date: 2020-03-27
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above detection methods all require special equipment and professional training personnel, and can only be tested in a laboratory, which is expensive, complicated to operate, and time-consuming, and is not suitable for clinical screening, let alone on-site instant detection.

Method used

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  • Colloidal gold test strip for detecting hepatitis E virus IgG antibody and preparation method of colloidal gold test strip
  • Colloidal gold test strip for detecting hepatitis E virus IgG antibody and preparation method of colloidal gold test strip
  • Colloidal gold test strip for detecting hepatitis E virus IgG antibody and preparation method of colloidal gold test strip

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Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Preparation of colloidal gold test strips for the detection of hepatitis E virus IgG antibodies

[0027] This colloidal gold test strip for detecting hepatitis E virus IgG antibody includes reaction support 5, absorbent paper pad 1, nitrocellulose membrane 2, colloidal gold pad 3, and gold-labeled antigen protective membrane 4. The colloidal gold pad contains colloid Gold-labeled glass fiber membrane of hepatitis E virus ORF2 protein, nitrocellulose membrane with two bands coated with goat anti-human IgG antibody and rabbit anti-HEV polyclonal antibody; the reaction support is 6cm×0.4cm PVC plastic sheet (purchased from Shanghai Jinbiao Biotechnology Co., Ltd., SM31-40); absorbent paper pad is 2.5 × 0.4cm filter paper (purchased from Shanghai Jinbiao Biotechnology Co., Ltd., CH37K); 2 cm × 0.4 cm of nitrocellulose Plain film (purchased from Shanghai Jiening Biotechnology Co., Ltd.), goat anti-human IgG antibody (purchased from Shanghai Biyuntian Biotechnology...

Embodiment 2

[0055] Embodiment 2: detection method

[0056] Collect whole blood and separate serum according to routine clinical methods. If the sample cannot be tested within three days, it should be stored at -20°C; take no less than 150-200 μl of serum in a small bottle, and put the test strip prepared in the above example with gold. Insert one end of the antigen-labeled protective film 4 into the serum in the bottle, the liquid in the sample goes up, and the result is read after 10-15 minutes;

[0057] If the sample contains hepatitis E virus IgG antibody, when it passes through the colloidal gold pad, it will bind to the gold-labeled HEVORF2 protein, and then be captured by the goat anti-human IgG antibody at T, and aggregate to form a red detection line, T line and C line color is positive ( image 3 ), only C line color is negative ( Figure 4 ); Regardless of whether the sample contains the antigen to be detected, the gold-labeled HEVORF2 protein will combine with the rabbit anti...

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Abstract

The invention discloses a colloidal gold test strip for detecting a hepatitis E virus IgG antibody. The colloidal gold test strip comprises a reaction support, an absorbent paper pad, a nitrocellulosemembrane, a colloidal gold pad and a gold-labeled antigen protection membrane, wherein the colloidal gold pad is a glass fiber membrane containing colloidal gold-labeled hepatitis E virus ORF2 protein, and the nitrocellulose membrane is provided with two strips coated with a goat anti-human IgG antibody and a rabbit anti-HEV polyclonal antibody. According to the invention, the hepatitis E virus IgG antibody can be detected, and the method is simple, convenient, quick and accurate, does not need the special instruments and devices and the professional training, is clear in results and simple in operation, is easy to distinguish the results, is easy to popularize, is suitable for large-batch detection, is suitable for grassroots screening and epidemiological investigation, can play an auxiliary diagnosis role in the early stage and the middle stage of hepatitis E virus infection, and has the reference significance in judging whether a human body is infected with hepatitis E virus antigens recently or not.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a colloidal gold test strip for detecting hepatitis E virus IgG antibody and a preparation method. Background technique [0002] Human hepatitis E virus (Hepatitis E Virus, HEV) is a pathogen that is susceptible to infection in young and middle-aged people worldwide. Immunocompromised patients, the elderly, and pregnant women are also susceptible to HEV. Hepatitis E virus has high infection and morbidity rates among pregnant women in any country. [0003] After the human body is infected with HEV, specific IgG antibodies are produced. Therefore, the rapid detection of hepatitis E virus IgG antibodies in human blood can be used as the preliminary basis for the clinical diagnosis of HEV infection and the prognosis of HEV infection. [0004] However, the current clinical diagnosis methods for HEV are mainly through virus isolation and culture, indirect immu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/56983G01N33/558
Inventor 黄芬李娅南闫自访禹文海杨臣臣陶余霞孙艺珈铁盛娇
Owner KUNMING UNIV OF SCI & TECH
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