Test strip for detecting enterovirus 71 (EV71) IgM antibody

A technology for testing test paper and test strips, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of easy promotion, clear and easy to distinguish results, and simple operation

Active Publication Date: 2011-11-23
辽宁迪浩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, so far, there is no report on a test strip that can detect EV71-specific IgM antibodies conveniently and quickly.

Method used

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  • Test strip for detecting enterovirus 71 (EV71) IgM antibody
  • Test strip for detecting enterovirus 71 (EV71) IgM antibody
  • Test strip for detecting enterovirus 71 (EV71) IgM antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: EV71-specific IgM antibody detection test strip (colloidal gold method) (see Figure 1)

[0029] EV71 genetically engineered antigen VP1 was purchased from Beijing Anbot Gene Technology Co., Ltd.

[0030] The reaction support is a 6.5cm×0.4cm PCV plate; the absorbent pad is a 2cm×0.4cm oil filter paper; the nitrocellulose membrane of 2.0cm×0.4cm is coated with anti-mouse IgG (2mg / ml), EV71 antigen VP1 (1.5mg / ml), containing the monoclonal antibody polyester film of 0.4cm × 0.4cm colloidal gold-labeled anti-human IgM (labeled amount 25 μg / ml); the glass fiber of sample pad 2.7cm × 0.4cm; that is, composed of enterovirus 71 Type (EV71) IgM antibody test strip (colloidal gold method).

Embodiment 2

[0031] Embodiment 2 detection method (see figure 2 )

[0032] 100-150 μl of the patient's whole blood, plasma or serum sample is directly dropped into the "4" of the test strip of Example 1, the sample solution runs up the membrane, and the result is interpreted within 10-15 minutes.

[0033] result:

[0034] If the test sample contains IgM antibody to EV71, it will form a corresponding complex with the colloidal gold-labeled anti-human IgM monoclonal antibody on the test strip, and then combine with the EV71-specific antigen VP1 coated on the nitrocellulose membrane to form a complex. A red line, i.e. a red band is formed at T.

[0035] Regardless of whether the specimen contains the corresponding antibody or not, the colloidal gold-labeled anti-human IgM monoclonal antibody continues to crawl upward and forms a red precipitation line with the anti-mouse IgG coated on the membrane, that is, a red band is formed at "C". This line is a quality control line. If the colloidal ...

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Abstract

The invention provides a rapid test strip for detecting an enterovirus 71 (EV71) IgM antibody. In the invention, EV71 gene antigenic vascular adhesion protein-1 (VAP1) and anti-mouse IgG are coated on a nitrocellulose (NC) membrane in combination with a colloidal gold labeled anti-human IgM monoclonal antibody, and the specific EV71 IgM antibody in an infected human specimen is detected by utilizing a membrane chromatography capture method. The rapid test strip provided by the invention has the advantages of being simple, convenient and rapid in operation, having no need of special instrumentand equipment and professional training, ensuring clear and easily-distinguished results and being easy for popularization; and the rapid test strip is applicable to a basic level as well as field test and early diagnosis, and plays a role in assisting the EV71 infection diagnosis.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to an enterovirus 71 (EV71) IgM antibody detection test strip and an application thereof. Background technique [0002] Enterovirus 71 (EV71) belongs to Enterovirus type A and is a close relative of Coxsackie virus A16 (CA16). EV71 infection can cause hand, foot and mouth disease (HFMD), encephalitis (encephalitis) , aseptic meningitis (asepicmeningitis) and poliomyelitis-like paralysis (poliomyelitis-like paralysis) and other diseases related to the nervous system. At present, humans are the only known natural host of EV71 virus, and the virus is mainly spread through close contact between people. According to the differences in nucleotide sequences, EV71 can be divided into three genotypes: A, B, and C. Types B and C can be further divided into subtypes B1, B2, B3, B4, and C1 and C2. The 7 strains of EV71 virus (SHZH03, SHZH98, SH2F1, SH2F2, SH2H25, SH2H26 and CHN287) that ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 刘明
Owner 辽宁迪浩生物科技有限公司
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