Colloidal gold test paper strip for detecting respiratory syncytial virus antibody and preparation method thereof

A syncytial virus and test strip technology, which is applied to the detection test strip of respiratory syncytial virus antibody colloidal gold and the field of preparation thereof, can solve the problems of complicated operation, high cost, long time consumption, etc. Easy to promote, simple effect

Inactive Publication Date: 2011-11-23
KUNMING BEIER ZUNSHENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above detection methods all require special equipment and professional training personnel, and can only be tested in a laboratory, which is expensive, complicated to operate, and time-consuming, and is not suitable for clinical screening, let alone on-site instant detection.

Method used

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  • Colloidal gold test paper strip for detecting respiratory syncytial virus antibody and preparation method thereof
  • Colloidal gold test paper strip for detecting respiratory syncytial virus antibody and preparation method thereof
  • Colloidal gold test paper strip for detecting respiratory syncytial virus antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] ——The composition of the colloidal gold detection strip for respiratory syncytial virus antibody

[0027] The reaction support is 6cm×0.4cm PVC rubber plate (purchased from Shanghai Jinbiao Biotechnology Co., Ltd., SM31-40); the absorbent paper pad is 2.5×0.4cm filter paper (purchased from Shanghai Jinbiao Biotechnology Co., Ltd., CH37K) 2cm×0.4cm nitrocellulose membrane (purchased from whatman company) was coated with anti-human IgA (purchased from KPL company) and anti-respiratory syncytial virus recombinant protein-specific polyclonal antibody (purchased from ABCAM company); containing 0.8 cm×0.4cm colloidal gold-labeled respiratory syncytial virus-specific recombinant antigen glass fiber (purchased from Shanghai Jinbiao Biotechnology Co., Ltd., SB03); gold-labeled antigen protective film 1cm×0.4cm polyester film (purchased from Shanghai Gold Standard Biotechnology Co., Ltd.), that is, formed a colloidal gold detection test strip for respiratory syncytial virus antib...

Embodiment 2

[0029] - How to prepare test strips

[0030] The nitrocellulose membrane pre-coated with anti-human IgA and anti-respiratory syncytial virus recombinant polyclonal antibody strips, colloidal gold-labeled respiratory syncytial virus recombinant protein antigen glass fiber, absorbent paper pad, gold-labeled antigen The protective film is pasted on the PVC rubber sheet in turn, leaving an overlap of about 0.2mm between each other, and pressed firmly to avoid leaving air bubbles. Cut the assembled nitrocellulose membrane into strips with a width of 0.4-0.6 cm with a strip cutter, and store them in an aluminum foil bag containing a desiccant at a relative humidity of 40-80% at 4°C for later use.

Embodiment 3

[0032] --Detection method

[0033] Take blood and separate serum according to clinical routine, or take sputum and throat swabs. If the samples cannot be tested within three days, they should be stored at -20°C. Take 150-200ul of serum or sputum, throat swab in a small bottle, insert the test strip "4" of Example 1 into the bottle, the liquid in the sample goes up, if there is no liquid crawling at "2" within 30 seconds , you should add 1-2 drops of normal saline or PBS buffer at "4". 10-15 minutes to interpret the results: if the sample contains RSV IgA antibody, when it passes through the colloidal gold pad, it will bind to the gold-labeled recombinant RSV antigen, and then be captured by the anti-human IgA at the T, aggregate and form If a red test line is formed, it is positive, otherwise it is negative. For details, please refer to the attached figure 2 .

[0034] Regardless of whether the sample contains the antibody to be detected, the gold-labeled recombinant anti...

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Abstract

The invention provides a colloidal gold test paper strip for detecting a respiratory syncytial virus (RSV) antibody. In the colloidal gold test paper strip, the IgA antibody of the respiratory syncytial virus is detected by enveloping an antihuman IgA polyclonal antibody on a nitrocellulose membrane (NC membrane), and combining a respiratory syncytial virus specific recombinant antigen marked by colloidal gold and by using immune chromatography technology. The IgA antibody of the respiratory syncytial virus can be detected simply, conveniently, quickly and accurately by using the test paper strip, and a detection result is clear and easy to distinguish, so the test paper strip is suitable for batch detection and is suitable for grass-root screening and epidemiological survey, plays the role in assisting diagnosis of the early stage and medium stage of the infection of the respiratory syncytial virus and has a reference significance for judging whether children have the immunity of RSV resistance or not.

Description

technical field [0001] The invention belongs to the field of biological detection, and more specifically, the invention relates to a detection test strip of respiratory syncytial virus antibody colloidal gold and a preparation method thereof. Background technique [0002] Human respiratory syncytial virus (Respiratory Syncytial Virus, RSV) is the most important pathogen of lower respiratory tract infection in infants and young children worldwide, and immunocompromised patients and the elderly are also susceptible to RSV. About 90,000 infants and young children are hospitalized each year due to RSV infection in the United States, with a mortality rate of 2% to 5%. About 15 million babies are born in my country every year, and more than 90% of children under the age of 2 have been infected with RSV. RSV has high rates of infection and morbidity among children in any country. [0003] After the incubation period of RSV infection, IgA antibodies appeared in the serum about 7 d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/544
Inventor 魏海涛赵军
Owner KUNMING BEIER ZUNSHENG TECH
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