Method for making recombinant antigen pp 65 envelope enzyme-linked reaction plate and ELISA test kit

Abstract

The present invention relates to a new method for making recombinant antigen PP65 coated enzyme labeled reaction plate and ELISA kit. Its key technique mainly liesin that using gene engineering method to prepare specific recombinant antigen-HCMV pp65, after the antigen is exracted and purified, using the coating plate as important component in the kit, besides, after the anti-human IgM monoclone antibody prepared by utilizing mouse-mouse hybridome technique is labeled by using horseradish peroxidase, it can be used for assembly of kit. Said method capable of creating sensitive quick ELISA antibody to trap HCMV specific IgM antibody in serum has high specificity and high stability, and said kit mainly can be used in laboratory research and clinical diagnosis.

Description

technical field

[0001] The invention relates to a novel biological preparation HCMVpp65 recombinant protein, which is an ELISA detection kit for detecting HCMV-IgM. Background technique

[0002] Human cytomegalovirus (Human Cytomegalovirus, HCMV) belongs to the human herpesvirus beta subfamily virus, the infection in the population is very common, showing a worldwide distribution. Serological epidemiological surveys show that 40-100% of adults have HCMV antibodies; different countries, different economic conditions, different groups of people, different age groups, HCMV infection rates are different, and its prevalence has no seasonal tendency. In Asia and Africa, 90% of the population is infected, and most of them have antibodies in their adolescence, indicating that the age of infection is earlier, while the infection occurs later in Western countries; the infection rate of people with low economic income is higher than that of people with higher economic income. HCMV is ...