A homogeneous immunoassay kit for detecting target igm antibody in a sample and its use method and application

A technology of samples and kits, applied in the field of biomedical testing, can solve problems such as false positives, lower work efficiency, false negatives, etc., and achieve the effect of solving influence and removing interference
CN107976535BActive Publication Date: 2020-03-20CHEMCLIN DIAGNOSTICS CO LTD
16 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHEMCLIN DIAGNOSTICS CO LTD
Publication Date
2020-03-20

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a homogeneous immunoassay kit for detecting a target IgM antibody in a sample, its use method and application. The kit includes reagent a, which includes a known antigen, which can specifically bind to the target IgM antibody in the sample to be tested to form a first complex; reagent b, which includes complement C1q, and the complement C1q can specifically binding to the first complex to form a second complex, and the complement C1q cannot bind to free IgM antibodies; wherein, any one of the known antigen and complement C1q is connected to a receptor; The acceptor can react with the received singlet oxygen to generate a detectable chemiluminescent signal; the reagent c includes a donor, and the donor can generate singlet oxygen in an excited state. The method for detecting the target IgM antibody in the sample by using the above-mentioned kit solves the impact of the non-specific IgM antibody on the detection.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a homogeneous immunoassay kit for detecting target IgM antibodies in a sample and its use method and application. Background technique

[0002] The detection of IgM antibodies is of great significance in medical testing. When a pathogen first infects the body, the first antibody produced is always IgM antibody. With the progress of the body's immune response, antibody class switching occurs to produce IgG, IgM or IgA antibody. Therefore, in the diagnosis of infectious diseases, a positive IgM antibody test is a sign of current infection.

[0003] At present, the commonly used IgM antibody detection method is the capture method, which is to coat the anti-human IgM (μ chain) antibody on the solid phase carrier, and after the serum / plasma sample reacts with the solid phase carrier, all the IgM antibody in the sample Captured on the solid phase carrier, aft...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More