A homogeneous immunoassay kit for detecting target igm antibody in a sample and its use method and application

Abstract

The invention relates to a homogeneous immunoassay kit for detecting a target IgM antibody in a sample, its use method and application. The kit includes reagent a, which includes a known antigen, which can specifically bind to the target IgM antibody in the sample to be tested to form a first complex; reagent b, which includes complement C1q, and the complement C1q can specifically binding to the first complex to form a second complex, and the complement C1q cannot bind to free IgM antibodies; wherein, any one of the known antigen and complement C1q is connected to a receptor; The acceptor can react with the received singlet oxygen to generate a detectable chemiluminescent signal; the reagent c includes a donor, and the donor can generate singlet oxygen in an excited state. The method for detecting the target IgM antibody in the sample by using the above-mentioned kit solves the impact of the non-specific IgM antibody on the detection.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a homogeneous immunoassay kit for detecting target IgM antibodies in a sample and its use method and application. Background technique [0002] The detection of IgM antibodies is of great significance in medical testing. When a pathogen first infects the body, the first antibody produced is always IgM antibody. With the progress of the body's immune response, antibody class switching occurs to produce IgG, IgM or IgA antibody. Therefore, in the diagnosis of infectious diseases, a positive IgM antibody test is a sign of current infection. [0003] At present, the commonly used IgM antibody detection method is the capture method, which is to coat the anti-human IgM (μ chain) antibody on the solid phase carrier, and after the serum / plasma sample reacts with the solid phase carrier, all the IgM antibody in the sample Captured on the solid phase carrier, aft...

AI Technical Summary

Problems solved by technology

This method has the following two disadvantages: one is that the anti-human IgM antibody is used as a capture antibody and cannot distinguish between specific IgM antibodies and non-specific IgM antibodies. When the concentration of specific IgM antibodies is low and the concentration of non-specific IgM antibodies is particularly high, it may Presence of specific IgM antibodies is removed by washing, resulting in false negative results

The second is that the detection process requires two steps of incubation and washing. In the washing process, it is very dependent on the washing efficiency of the washing equipment, which is prone to problems such as poor repeatability. The two incubation steps are cumbersome and time-consuming, which reduces work efficiency.

However, this method adds a new biologically active substance, and there are too many protein components in the reaction system, which may cause false positives

In addition, non-specific IgM antibodies in the homogeneous reaction system will also consume a large amount of anti-human IgM antibodies. When the concentration of specific IgM antibodies is low and the concentration of non-specific IgM antibodies is particularly high, it may also cause false negatives.

Images