Colloidal gold chromatographic band for joint detecting specific IgM. IgG antibody and producing method thereof

A joint detection and specificity technology, applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problems of inconvenient and fast detection, cumbersome operation process, etc., and achieve the effect of simplifying the operation process and high overall coincidence rate

Active Publication Date: 2007-08-22
北京英诺特生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the individual detection of IgM and IgG antibodies, and then a comprehensive analysis of the test results has no effect on the diagnosis of the disease, but the operation process is cumbersome and the detection is not convenient and quick

Method used

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  • Colloidal gold chromatographic band for joint detecting specific IgM. IgG antibody and producing method thereof
  • Colloidal gold chromatographic band for joint detecting specific IgM. IgG antibody and producing method thereof
  • Colloidal gold chromatographic band for joint detecting specific IgM. IgG antibody and producing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preparation method of the colloidal gold chromatography strip that detects human cytomegalovirus (HCMV) IgM, IgG antibody

[0019] Concrete preparation process is as follows:

[0020] (1) The HCMV antigen with a concentration of 3mg / ml was prepared by the gene recombination method, the rabbit anti-HCMV antibody with a concentration of 5mg / ml was prepared by the animal immunization method, and the mouse anti-human IgM monoclonal with a concentration of 2mg / ml was prepared by the monoclonal antibody preparation method Antibody, spare.

[0021] (2) Preparation of antigen-colloidal gold complex: Colloidal gold was prepared by trisodium citrate reduction method, and purified HCMV antigen was added to the gold sol at a ratio of 80 μg / ml. After sealing and centrifugation, the precipitate was diluted to A 530 value 2.0, spare;

[0022] (3) Coating with mouse anti-human IgM monoclonal antibody, HCMV antigen, and rabbit anti-HCMV antibody: Take a nitrocellulos...

Embodiment 2

[0026] Embodiment 2: detect the detection result of human cytomegalovirus (HCMV) IgM, IgG antibody

[0027] The prepared colloidal gold chromatography strip was used to detect 383 anti-HCMV IgG positive sera and 1979 negative sera with definite clinical diagnosis, and compared with the results of the indirect method ELISA reagent alone to detect anti-HCMV IgG. The results are shown in Table 1 and Table 2.

[0028] Table 1 Colloidal gold chromatographic strip detection results of anti-HCMV IgG clinical serum

[0029] Table 2 Comparison of the results of colloidal gold and ELISA detection of anti-HCMV IgM

[0030] From the data in Table 1, the Youden index can be calculated as 362 / (362+21)+1977 / (2+1977)-1=0.94, indicating that the overall coincidence rate between the test results and the real situation of the samples is very high. From the data in Table 2, the overall coincidence rate between the detection results of the present invention and the ELISA detection results ca...

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Abstract

The invention provides a colloidal gold chromatography band and the preparing technology to detect the special IgM,IgG. The colloidal gold is as the labeled thing of the colloidal gold. One end of the PVC back plate is pasted with the sample pad, the compound pad, the nitryl fibrous membrane, the other end is pasted with the absorbing pad; the compound pad is the glass fibrous membrane covered with the antigen-colloidal gold compound. The character is in that: the fibrous membrane includes three cover lines which cover the anti IgM antibody, the special antigen and the antibody according to the antigen separately. The invention detects the IgM antibody using the immune capture method principle and detects the IgG antibody by the double antigen sandwich method. So it can detect the special IgM, IgG antibody by one operation and the result has the high whole according ratio.

Description

technical field [0001] The invention relates to a chromatographic strip for detecting specific IgM and IgG antibodies by means of an immunoassay method and a preparation technology thereof, in particular to an immunochromatographic reaction for specific IgM and IgG antibodies by means of colloidal gold labeling for color development. A chromatographic strip for combined detection and a preparation method thereof. Background technique [0002] Immunoglobulin M (Immunoglobulin M, IgM) and Immunoglobulin G (Immunoglobulin G, IgG) are the two most important antibodies in humans or animals. After an antigen (bacteria or virus, etc.) enters the body, the first immunoglobulin that appears is IgM antibody, and then IgG antibody appears. Although IgA antibody may also appear in some cases, because its clinical significance is basically the same as that of IgG, and the IgA content is very low, it has little practical significance in clinical testing, so it is generally not distinguis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/532G01N33/577
Inventor 王健陈廷友王志新翟立伟孔祥菊毕大伟张秀杰
Owner 北京英诺特生物技术股份有限公司
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