Western blot kit for cardiolipin and specific IgM antibodies of syphilis and preparation thereof

Abstract

The invention provides a western blot kit for cardiolipin and specific IgM antibodies of syphilis and preparation thereof, relating to a kit. The kit is provided with a carrier plate, a cellulose nitrate membrane, a detection line of the specific IgM antibodies and cardiolipin IgM antibodies of the syphilis and a control line, wherein the detection line and the control line are arranged on the cellulose nitrate membrane in sequence; specific recombinant antigens and cardiolipin antigens of the syphilis are coated at the detection line of the specific IgM antibodies of the syphilis, and human IgM antibodies are coated at the control line; and horse radish peroxidase is marked on anti-human mu-chain antibodies. The preparation comprises the following steps: preparing the specific recombinant antigens of the syphilis, then preparing the cardiolipin antigens, carrying out sample application on the cellulose nitrate membrane, and preparing mu-chain monoclonal antibodies of anti-human IgM specific segments; and marking the horse radish peroxidase on the anti-human IgM specific segments mu-chain monoclonal antibodies, and then preparing the western blot kit. The kit provided by the invention can be used for detection of the specific IgM antibodies and cardiolipin antigens of the syphilis in specimens such as whole blood, blood serum, blood plasma, cerebrospinal fluid and the like.

Description

Syphilitic cardiolipin and specific antibody IgM immunoblotting kit and its preparation technical field The invention relates to a kit, in particular to a syphilis-specific IgM antibody and cardiolipin IgM antibody immunoblotting kit and a preparation method thereof. Background technique Syphilis is a sexually transmitted disease caused by Treponema pallidum (TP), and its pathogen is Treponema pallidum, which belongs to the family Treponemaceae. Treponema pallidum is mainly transmitted through sexual contact, blood transfusion, wound or placenta. Treponema pallidum enters the blood from the lymph nodes near the infected area, spreads throughout the body, and affects almost all tissues and organs of the body. The World Health Organization (WHO) once predicted optimistically: "Due to the availability of highly sensitive detection methods and efficient treatment options, syphilis is a sexually transmitted disease that can be successfully controlled through public health meas...

AI Technical Summary

Problems solved by technology

The commercially available Western-blot reagents are all imported reagents, which are expensive

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