Kit and method for detecting novel coronavirus SARS-CoV-2 antibody

A coronavirus, sars-cov-2 technology, applied in the field of biomedical testing, can solve the problems of indistinguishable, cumbersome and complicated testing process, false positives, etc.

Inactive Publication Date: 2021-08-24
MEDCAPTAIN MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection process recommended by the clinical inspection center is quite cumbersome and complicated, and the general antibody detection method is susceptible to some substances in blood samples (such as rheumatoid factor, non-specific IgM / IgG (such as those affected by other types of coronaviruses, influenza viruses, adenoviruses, etc.) "False positive" results due to the interference of antibodies produced by viruses and other infections, etc., making it difficult to distinguish whether the infection is caused by the recent new coronavirus (SARS-CoV-2) or other viruses

Method used

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  • Kit and method for detecting novel coronavirus SARS-CoV-2 antibody
  • Kit and method for detecting novel coronavirus SARS-CoV-2 antibody

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Embodiment Construction

[0046] The following description is an exemplary embodiment of the present invention, and it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also It is regarded as the protection scope of the present invention.

[0047] Unless otherwise specified, the chemical reagents involved in the following description are all commercially available reagents.

[0048] Magnetic beads coated with SARS-CoV-2 antigen

[0049] The process of linking specific antigens to magnetic beads by means of covalent coupling is called magnetic bead coating, and the coated product can become magnetic particle coating. The basic principle of coating is as follows: the chemical groups (carboxyl, amine, p-toluenesulfonyl, etc.) on the surface of the magnetic beads chemically react with the amine or carboxyl on the surface of the antigen to ...

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Abstract

The invention provides a kit for detecting a novel coronavirus SARS-CoV-2 antibody. The kit comprises magnetic particles coated with an SARS-CoV-2 antigen, an anti-human IgM / IgG secondary antibody labeled by a chemiluminescent marker, and a non-novel coronavirus antigen. By adopting the kit, whether a patient sample to be detected contains a specific IgM / IgG antibody generated by infection of the novel coronavirus SARS-CoV-2 or not can be specifically detected, and the specific IgM / IgG antibody does not generate cross reaction with influenza virus, common coronavirus and the like. The invention also provides a detection method of the novel coronavirus.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a kit and method for detecting antibodies against novel coronavirus SARS-CoV-2. Background technique [0002] The novel coronavirus (SARS-CoV-2) directly attacks the patient's respiratory system and digestive system, causing the patient's immune system to be disturbed, and in severe cases, an inflammatory storm will result in death. [0003] One of the main reasons why the new coronavirus is so harmful is that patients have an incubation period of 3-14 days or even longer. Early patients do not have any symptoms, even if the widely accepted gold standard fluorescent quantitative PCR technology is used, the positive detection rate in the early stage of the disease is only 30-50%, making the diagnosis extremely difficult. Medical institutions have to use CT and clinical symptoms as supplementary means of nucleic acid testing, which not only costs a lot of money, takes a long ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/543G01N21/76
CPCG01N33/6854G01N33/56983G01N33/54326G01N21/76G01N2333/165G01N2469/20
Inventor 余枝广肖良品柴凡郭媛媛吴晓丽崔易航吴轩德
Owner MEDCAPTAIN MEDICAL TECH
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