Microfluidic optical sensor real-time hematopoietic stem cell detection device

Abstract

The invention discloses a micro-fluidic optical sensor real-time hematopoietic stem cell detection device which comprises a micro-fluidic chip, an optical sensing system and a data processing unit.The micro-fluidic chip is used for guiding a blood sample, an optical detection mode of optical turbidity measurement is combined, the detection speed and the detection accuracy are increased, and the detection accuracy is improved. The concentration of the hematopoietic stem cells can be monitored in real time, instant data are provided, and clinical instant decision making is supported. The method is simple and convenient to operate, does not need complex equipment or a long-time treatment process, and is suitable for a POCT environment. By combining a microfluidic technology and an optical sensor, the detection accuracy of the cell concentration is improved, and the influence of interference factors is reduced. Compared with flow cytometry equipment, the device is simple in structure and can be designed in a miniaturized manner, so that the device is suitable for clinical sites or other instant detection environments. And by using the micro-fluidic chip and the low-energy-consumption optical sensor, the influence on the environment is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a microfluidic optical sensor real-time hematopoietic stem cell detection device. Background Art

[0002] At present, the detection of hematopoietic stem cells mainly relies on flow cytometry and optical turbidity measurement. However, its shortcomings are obvious during long-term use. The main shortcomings are as follows:

[0003] Flow cytometry: Flow cytometry is a commonly used cell analysis technique that allows cells to pass through a laser beam one by one in a liquid flow, and measures the light scattering and fluorescence properties of the cells to obtain the number and characteristics of cells. This method has the advantages of high precision and high throughput, and is suitable for cell counting and sorting. However, flow cytometry usually requires complex equipment and a long operation time, and is not suitable for rapid, on-site detection.

[0004] Optical turbidity measurement: Optical turbidity measurement uses light scattering or transmission to estimate the concentration of cells in a sample. This method is generally simple and suitable for rapid analysis of liquid samples. However, the accuracy of this method can be affected by other components in the sample and may not accurately distinguish different types of cells.

[0005] To solve the above problems, a microfluidic optical sensor real-time hematopoietic stem cell detection device is proposed. Summary of the invention

[0006] The purpose of the present invention is to provide a microfluidic optical sensor real-time hematopoietic stem cell detection device to solve the problems raised in the above background technology.

[0007] To achieve the above object, the present invention provides the following technical solution: a microfluidic optical sensor real-time hematopoietic stem cell detection device,

[0008] Includes: microfluidic chip, optical sensing system and data processing unit;

[0009] The microfluidic chip introduces a blood sample to be tested, guides the sample to mix with a reagent, and guides the sample to an analysis and processing position.

[0010] The optical sensor system irradiates the mixed sample at the analysis and processing location of the microfluidic chip and converts the optical signal into an electrical signal;

[0011] The data processing unit processes the electrical signals from the optical sensor system, makes judgments on the data, and displays real-time data and collection suggestions.

[0012] The microfluidic chip comprises a sample input port, a microfluidic channel, a mixing area and an analysis area, and the sample input port, the microfluidic channel, the mixing area and the analysis area are integrated together;

[0013] The sample input port is connected to the entrance of the microfluidic channel, and the sample input port introduces the blood sample to be tested, and introduces the blood sample to be tested into the microfluidic channel. The microfluidic channel guides the blood sample to be tested to the mixing area and the analysis area in sequence, and the mixing area mixes the blood sample with the reagent.

[0014] Preferably, the reagent mixed with the sample in the mixing zone is a hematopoietic stem cell marker.

[0015] Preferably, the optical sensing system includes a light source, a photodetector and an optical lens. The light source irradiates the blood sample in the analysis area. The photodetector receives the light scattered or transmitted by the light source on the cells in the blood sample and focuses the light onto the photodetector through the optical lens. The photodetector converts the light signal into an electrical signal and transmits the electrical signal to the data processing unit.

[0016] Preferably, the optical sensing system further includes a light source control system for controlling the switching and brightness of the light source.

[0017] Preferably, the light source is a laser or an LED.

[0018] Preferably, the data processing unit includes a signal processing unit and a display unit, wherein the signal processing unit processes the electrical signal converted by the photodetector, calculates the cell concentration and determines whether the collection bar is reached, and displays real-time data and collection suggestions through the display unit.

[0019] Preferably, the signal processing unit includes a signal processing module and a data processing module.

[0020] Preferably, the signal processing module includes a signal amplifier and a filter. The signal amplifier amplifies the electrical signal converted by the photodetector, performs filtering processing through the filter, and transmits the signal to the data processing module for processing.

[0021] Preferably, the data processing module includes a microprocessor and a memory bar, and the microprocessor processes and judges the signal transmitted by the signal amplifier and displays it on the display unit, and stores the data in the memory bar.

[0022] Compared with the prior art, the beneficial effects of the present invention are: a microfluidic optical sensor real-time hematopoietic stem cell detection device,

[0023] Real-time monitoring:

[0024] The present invention utilizes a microfluidic chip to guide blood samples and combines it with an optical detection method of optical turbidity measurement to speed up the detection speed and accuracy, and can monitor the concentration of hematopoietic stem cells in real time, provide instant data, and support clinical instant decision-making.

[0025] Comparison: In the existing technology, flow cytometry and other detection methods usually require a long processing time and are not suitable for real-time detection, while the optical detection method of optical turbidity measurement has the problem of large interference and low accuracy.

[0026] Easy operation:

[0027] Advantages: Easy to operate, does not require complicated equipment or long processing procedures, and is suitable for POCT environments.

[0028] Comparison: Traditional testing equipment is usually complex to operate and requires specialized laboratory conditions and personnel training.

[0029] High accuracy:

[0030] Advantages: Combining microfluidics technology and optical sensors improves the accuracy of cell concentration detection and reduces the impact of interfering factors.

[0031] Comparison: The optical turbidity measurement in existing technologies may be interfered by other components of the sample, while microfluidics technology can effectively control the sample processing conditions.

[0032] Portability:

[0033] Compared with flow cytometry equipment, it has a simple structure and can be miniaturized, making it suitable for clinical sites or other instant testing environments.

[0034] Environmentally friendly:

[0035] Advantages: Use of microfluidic chips and low-energy optical sensors reduces environmental impact.

[0036] Comparison: Traditional testing equipment may require more chemical reagents and resources, increasing the environmental burden. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is a flow chart of the present invention;

[0038] Figure 2 It is an overall schematic diagram of the present invention;

[0039] Figure 3 Schematic diagram of the microfluidic chip of the present invention;

[0040] Figure 4 Schematic diagram of the optical sensing system of the present invention;

[0041] Figure 5 Schematic diagram of the data processing unit of the present invention.

[0042] In the figure: microfluidic chip 1, sample input port 11, microfluidic channel 12, mixing area 13, analysis area 14, optical sensing system 2, light source 21, photodetector 22, optical lens 23, data processing unit 3, signal processing unit 31, signal processing module 311, signal amplifier 311-1, filter 311-2, data processing module 312, microprocessor 312-1, memory bar 312-2 display unit 32. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] See also Figure 1-5 The present invention provides a technical solution: a microfluidic optical sensor real-time hematopoietic stem cell detection device,

[0045] It includes: a microfluidic chip 1, an optical sensing system 2 and a data processing unit 3;

[0046] The microfluidic chip 1 introduces the blood sample to be tested, guides the sample to mix with the reagent, and guides the sample to the analysis and processing position.

[0047] The optical sensing system 2 irradiates the mixed sample at the analysis and processing location of the microfluidic chip 1 and converts the optical signal into an electrical signal;

[0048] The data processing unit 3 processes the electrical signals from the optical sensor system 2, makes judgments on the data, and displays real-time data and collects suggestions.

[0049] Sample processing: Mix the blood sample with the reagent to ensure homogeneity.

[0050] Real-time monitoring:

[0051] The present invention utilizes the microfluidic chip 1 to guide blood samples and combines it with an optical detection method of optical turbidity measurement to speed up the detection speed and accuracy, and can monitor the concentration of hematopoietic stem cells in real time, provide real-time data, and support clinical real-time decision-making.

[0052] Comparison: In the existing technology, flow cytometry and other detection methods usually require a long processing time and are not suitable for real-time detection, while the optical detection method of optical turbidity measurement has the problem of large interference and low accuracy.

[0053] Easy operation:

[0054] Advantages: The present invention only requires the blood sample to be input from the sample input port 11, is easy to operate, does not require complex equipment or a long processing process, and is suitable for a POCT environment.

[0055] Comparison: Traditional testing equipment is usually complex to operate and requires specialized laboratory conditions and personnel training.

[0056] High accuracy:

[0057] Advantages: Combining microfluidic technology and optical sensors, using microfluidic chip 1 and optical sensor system 2 to cooperate, improves the detection accuracy of cell concentration and reduces the influence of interference factors.

[0058] Comparison: The optical turbidity measurement in existing technologies may be interfered by other components of the sample, while microfluidics technology can effectively control the sample processing conditions.

[0059] Portability:

[0060] Compared with flow cytometry equipment, it has a simple structure and can be miniaturized, making it suitable for clinical sites or other instant testing environments.

[0061] Environmentally friendly:

[0062] Advantages: Use of microfluidic chips and low-energy optical sensors reduces environmental impact.

[0063] Comparison: Traditional testing equipment may require more chemical reagents and resources, increasing the environmental burden.

[0064] Specifically, the microfluidic chip 1 includes a sample input port 11, a microfluidic channel 12, a mixing area 13 and an analysis area 14, and the sample input port 11, the microfluidic channel 12, the mixing area 13 and the analysis area 14 are integrated together;

[0065] The sample input port 11 is connected to the entrance of the microfluidic channel 12. The sample input port 11 introduces the blood sample to be tested, and introduces the blood sample to be tested into the microfluidic channel 12. The microfluidic channel 12 guides the blood sample to be tested to the mixing area 13 and the analysis area 14 in sequence. The mixing area 13 mixes the blood sample with the reagent.

[0066] Sample input port 11: The sample is introduced through this port, and the inlet design ensures that the sample smoothly enters the microfluidic channel 12.

[0067] Microfluidic channel 12: The interior of the channel includes a staggered flow area and a sharp turn area to enhance the sample processing effect.

[0068] Mixing zone 13: Ensure that the sample and reagent are fully mixed to improve the detection accuracy.

[0069] Analysis area 14: The light source 21 illuminates the sample, and the photodetector receives and analyzes the light signal to obtain cell concentration data.

[0070] Specifically, the reagent mixed with the sample in the mixing area 13 is a hematopoietic stem cell marker.

[0071] Specifically, the optical sensing system 2 includes a light source 21, a photodetector 22 and an optical lens 23. The light source 21 irradiates the blood sample in the analysis area. The photodetector 22 receives the light scattered or transmitted by the light source 21 on the cells in the blood sample and focuses the light onto the photodetector 22 through the optical lens. The photodetector 22 converts the optical signal into an electrical signal and transmits the electrical signal to the data processing unit 3.

[0072] The light source 21 is used to illuminate the sample flowing through the analysis area. The light source type can be laser or LED, and a suitable light source is selected to meet the detection requirements.

[0073] Light source 21: The light beam is focused by an optical lens to ensure that the light from the light source illuminates every part of the analysis area.

[0074] Photodetector 22: Receives light scattering or transmission signals of the sample after being illuminated by the light source. The photodetector 22 is located opposite to the light source to ensure accurate signal collection.

[0075] Photodetector 22: collects light signals passing through the sample and converts them into electrical signals for analysis by the data processing unit 3.

[0076] Specifically, the optical sensing system 2 further includes a light source control system, which controls the switching and brightness of the light source 21 .

[0077] Specifically, the light source 21 is a laser or an LED.

[0078] Specifically, the data processing unit 3 includes a signal processing unit 31 and a display unit 32. The signal processing unit 31 processes the electrical signal converted by the photodetector 22, calculates the cell concentration and determines whether the collection bar is reached, and displays real-time data and collection suggestions through the display unit 32.

[0079] Specifically, the signal processing unit 31 includes a signal processing module 311 and a data processing module 312 .

[0080] Specifically, the signal processing module 311 includes a signal amplifier 311 - 1 and a filter 311 - 2 . The signal amplifier 311 - 1 amplifies the electrical signal converted by the photodetector, performs filtering processing through the filter, and transmits the signal to the data processing module 312 for data judgment and processing.

[0081] Specifically, the data processing module 312 includes a microprocessor 312-1 and a memory bar 312-2. The microprocessor 312-1 processes and judges the signal transmitted by the signal amplifier 311-1, and displays it on the display unit 32, and stores the data in the memory bar 312-2.

[0082] Data processing module 312: amplifies and filters the electrical signal from the photodetector 22 to improve the signal quality.

[0083] The data processing module 312 calculates the cell concentration based on the processed signal and compares it with the preset acquisition standard.

[0084] Display unit 32: displays cell concentration data and whether the collection conditions are met in real time, so that users can make instant decisions.

[0085] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A microfluidic optical sensor real-time hematopoietic stem cell detection device, include: Microfluidic chip (1), optical sensing system (2) and data processing unit (3); The microfluidic chip (1) introduces a blood sample to be tested, guides the sample to mix with a reagent, and guides the sample to an analysis and processing position; The optical sensing system (2) irradiates the mixed sample at the analysis and processing location of the microfluidic chip (1) and converts the optical signal into an electrical signal; The data processing unit (3) processes the electrical signal from the optical sensor system (2), judges the data thereof, and displays real-time data and collection suggestions.

2. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The microfluidic chip (1) comprises a sample input port (11), a microfluidic channel (12), a mixing area (13) and an analysis area (14), wherein the sample input port (11), the microfluidic channel (12), the mixing area (13) and the analysis area (14) are integrated together; The sample input port (11) is connected to the entrance of the microfluidic channel (12); the sample input port (11) introduces a blood sample to be tested, and introduces the blood sample to be tested into the microfluidic channel (12); the microfluidic channel (12) guides the blood sample to be tested to the mixing area (13) and the analysis area (14) in sequence; the mixing area (13) mixes the blood sample with a reagent.

3. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The reagent mixed with the sample in the mixing area (13) is a hematopoietic stem cell marker.

4. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The optical sensing system (2) comprises a light source (21), a photodetector (22) and an optical lens (23). The light source (21) irradiates the blood sample in the analysis area. The photodetector (22) receives the light scattered or transmitted from the light source (21) on the cells in the blood sample and focuses the light onto the photodetector (22) through the optical lens. The photodetector (22) converts the light signal into an electrical signal and transmits the electrical signal to the data processing unit (3).

5. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The optical sensing system (2) further comprises a light source control system, wherein the light source control system controls the switching and brightness of the light source (21).

6. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The light source (21) is a laser or an LED.

7. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The data processing unit (3) comprises a signal processing unit (31) and a display unit (32). The signal processing unit (31) processes the electrical signal converted by the photodetector (22), calculates the cell concentration and determines whether the collection bar is reached, and displays real-time data and collection suggestions through the display unit (32).

8. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The signal processing unit (31) comprises a signal processing module (311) and a data processing module (312).

9. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The signal processing module (311) comprises a signal amplifier (311-1) and a filter (311-2); the signal amplifier (311-1) amplifies the electrical signal converted by the photodetector, performs filtering processing through the filter, and transmits the amplified electrical signal to the data processing module (312) for data judgment and processing.

10. A microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, Features: The data processing module (312) comprises a microprocessor (312-1) and a memory bar (312-2); the microprocessor (312-1) processes and judges the signal transmitted by the signal amplifier (311-1), displays the signal on the display unit (32), and stores the data in the memory bar (312-2).

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