Reagent strip for joint detection of syphilis specific IgM and IgG antibodies and preparation method thereof

A technology of antibody detection and combined detection, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of small sample size, low cost and high sensitivity

Inactive Publication Date: 2010-09-08
ZHONGSHAN HOSPITAL XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a negative TP-IgM does not completely rule out infectivity, a positive TP-IgM must suggest that the patient is infectious

Method used

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  • Reagent strip for joint detection of syphilis specific IgM and IgG antibodies and preparation method thereof
  • Reagent strip for joint detection of syphilis specific IgM and IgG antibodies and preparation method thereof
  • Reagent strip for joint detection of syphilis specific IgM and IgG antibodies and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The preparation method of the syphilis-specific IgM and IgG antibody combined detection reagent strip of the present invention comprises the following steps:

[0031] 1) Preparation of syphilis recombinant antigens TPN17 and TPN47: Using gene cloning technology, PCR amplified DNA encoding Treponema pallidum antigens and inserted into Escherichia coli for expression to obtain syphilis recombinant antigens TPN17 and TPN47.

[0032] 2) Spotting of nitrocellulose membrane: coating anti-human IgG specific fragment γ chain monoclonal antibody at the syphilis-specific IgG antibody detection line on the nitrocellulose membrane (NC membrane), The syphilis-specific IgM antibody detection line on the membrane) was coated with anti-human IgM specific fragment μ chain monoclonal antibody, and the control line on the nitrocellulose membrane (NC membrane) was coated with goat anti-syphilis antigens TPN17 and TPN47 IgG antibody, dried at room temperature, sealed at room temperature and...

Embodiment 2

[0042] Similar to Example 1, the difference is that the gold conjugate pad consists only of TPN17 and does not contain TPN47. Result judgment is identical with embodiment 1.

Embodiment 3

[0044] Similar to Example 1, the difference is that the gold conjugate pad consists only of TPN47 and does not contain TPN17. Result judgment is identical with embodiment 1.

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Abstract

The invention provides a reagent strip for joint detection of a syphilis specific IgM and IgG antibodies and a preparation method thereof, relating to a reagent for detection of a syphilis specific antibody. The invention provides the reagent strip for joint detection of the syphilis specific IgM and IgG antibodies and the preparation method thereof. The reagent strip is provided with a vector plate, a loading pad, a colloidal gold pad, a nitrocellulose membrane, a syphilis specific IgG antibody detection line, a syphilis specific IgM antibody detection line, a contrast line and an absorption pad. The preparation method comprises the following steps: preparing sample application of nitrocellulose membranes for recombinant syphilis antigens TPN17 and TPN47, preparing colloidal gold, labeling syphilis specific antigen TPN17 and TPN47 with colloidal gold and preparing the reagent strip for joint detection of the syphilis specific IgM and IgG antibodies.

Description

technical field [0001] The invention relates to a syphilis-specific antibody detection reagent, in particular to a syphilis-specific IgM and IgG antibody combined rapid detection reagent strip and a preparation method thereof by using a gold standard immunochromatography technique (immunochromatography). Background technique [0002] Syphilis (Syphilis) is a sexually transmitted disease caused by Treponema pallidum (TP), and its pathogen is Treponema pallidum, which belongs to the family Treponemaceae. Treponema pallidum is mainly transmitted through sexual contact, blood transfusion, wound or placenta. Treponema pallidum enters the blood from the lymph nodes near the infected area, spreads throughout the body, and affects almost all tissues and organs of the body. The World Health Organization (WHO) once predicted optimistically: "Due to the availability of highly sensitive detection methods and efficient treatment options, syphilis is a sexually transmitted disease that c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/571G01N33/543
Inventor 杨天赐林丽蓉张忠英张长弓
Owner ZHONGSHAN HOSPITAL XIAMEN UNIV
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