Reagent strip for carrying out joint detection on Toxoplasma gondii IgM (immunoglobulin M) and IgG (immunoglobulin G) antibodies and preparation method thereof

Abstract

The invention discloses a reagent strip for carrying out joint detection on Toxoplasma gondii IgM (immunoglobulin M) and IgG (immunoglobulin G) antibodies and a preparation method thereof, and relates to a toxoplasma gondii antibody detection reagent. The invention provides a reagent strip for carrying out joint detection on Toxoplasma gondii IgM (immunoglobulin M) and IgG (immunoglobulin G) antibodies and a preparation method thereof. The reagent strip is provided with a carrier board, a loading pad, a colloidal gold pad, a nitrocellulose membrane, a toxoplasma gondii IgG antibody detection line, a toxoplasma gondii IgM antibody detection line, a control line and an absorbing pad, The preparation method disclosed by the invention comprises the following steps: preparing toxoplasma gondii recombinant antigen SAG (supper antigen)1(P30), SAG2(P22), ROP2 and GRA7; carrying out sample application on the nitrocellulose membrane; preparing a colloidal gold; carrying out marking on the colloidal gold and the toxoplasma gondii antigen SAG1(P30),SAG2(P22),ROP2 and GRA7; and preparing the reagent strip for carrying out joint detection on toxoplasma gondii IgM (immunoglobulin M) and IgG (immunoglobulin G) antibodies.

Description

technical field [0001] The invention relates to a detection reagent for toxoplasma gondii antibody, in particular to a combined rapid detection reagent strip for toxoplasma gondii IgM and IgG antibody by using a gold standard immunochromatography technique (immunochromatography) and a preparation method thereof. Background technique [0002] Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasmagondii that seriously endangers human health. The pathogen Toxoplasma can parasitize in the nucleated cells of humans and various animals. and animals are generally susceptible. The disease is widely distributed all over the world. According to statistics, about 1 billion people in the world are infected by Toxoplasma gondii ([1] Xi Linlin, Li Wei. Research progress on pathogenic mechanism, virulence and genotype of Toxoplasma gondii [J]. Journal of Pathogen Biology, 2009, 4(11): 859-861.). Toxoplasmosis is widely distributed in my country, and there are reports of toxop...

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Problems solved by technology

[0003] The diagnosis method of toxoplasmosis involves isolating toxoplasma directly from organs, blood, cerebrospinal fluid and other tissues for etiological diagnosis, which takes days or even weeks, is time-consuming and laborious, and has little value in practical applications

Routine clinical detection mainly uses various serological methods to detect its specific IgG and IgM ([3] Zhou Bin, Zhang Jue, Wang Ke, et al. The establishment and implementation of double-labeled time-resolved fluorescence immunoassay for Toxoplasma gondii IgG and IgM antibodies Its preliminary clinical application [J]. Chinese Journal of Laboratory Medicine, 2010, 33 (010): 957-959.), serological methods can diagnose congenital, acute and chronic toxoplasmosis, but because most immunocompromised people or The anti-toxoplasma IgG titer of animals does not rise or does not appear high IgM titer, so serological methods cannot be used effectively to diagnose toxoplasmosis in immunocompromised humans or animals

In addition, the serum antibody titer will gradually decrease after the antigen disappears for a long time, so it cannot be used to evaluate the therapeutic effect

At present, there are high false positives in the detection of IgG antibodies, and the general sensitivity of IgM antibody detection is poor ([4] Han Jingyun, Liu Qian, Guo Jian. Technical progress in laboratory diagnosis of Toxoplasma gondii infection [J]. LABORATORY MEDICINE, 2009, 24(5).)

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