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Preparation method of microarray chip and product thereof

A microarray chip and chip technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problems of no high-throughput three-dimensional micro-environment, cumbersome operation, high cost, etc., to facilitate analysis And the effect of research and operation is simple and fast

Active Publication Date: 2015-12-23
黑玉星岩国际科学技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are relatively expensive and cumbersome to operate, and do not have high-throughput and high-efficiency assembly of complex three-dimensional microenvironments, so it is difficult to truly apply to industrial-level high-throughput screening.

Method used

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  • Preparation method of microarray chip and product thereof
  • Preparation method of microarray chip and product thereof
  • Preparation method of microarray chip and product thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0051] The preparation method of the basic chip is as follows:

[0052] (1) Design a photolithographic mask according to the morphology of the desired hydrophilic region and hydrophobic region; Similarly, when using a positive photoresist, such as SU-8, the shape of the mask is the same as that of the desired hydrophilic area;

[0053] (2) Utilize negative mold lithography or positive mold lithography to prepare a PDMS (polydimethylsiloxane) stamp with a corresponding shape, the PDMS stamp includes a raised portion and a depressed portion, and the raised portion The morphology of the depression corresponds to the hydrophobic region, and the morphology of the depression corresponds to the hydrophilic region;

[0054] (3) Treat the PDMS stamp and the substrate with plasma; wherein, the substrate surface is smooth and has a hydrophilic group; the hydrophilic group is a hydroxyl group, a carboxylic acid group, a sulfonic acid group, a phosphoric acid group, Amino groups, quatern...

Embodiment 1

[0072] The preparation of embodiment 1 glass substrate

[0073] Step 1: Make the positive mold by soft lithography technology

[0074] Throw the photoresist SU-8 (1070) on the cleaned and dried silicon wafer (700r18s, 2500r60s), and pre-baked to remove the solvent in the SU-8 glue (65°C 15min, 95°C 2hour), so that SU-8 The positive mold is better bonded to the silicon wafer, and then photolithography (3.5mJ / cm 2 ), the mask plate used in lithography is set according to the shape of the positive mold, and the photolithography time is 60s; then it is placed on a hot plate for post-baking (65°C 15min, 95°C 2hour), so that the positive mold and The silicon wafer is more closely bonded, and after being developed by a developer, the film is hardened (135°C) for more than 1 hour to achieve the effect of close adhesion between SU-8 and the silicon wafer, and the SU-8 anode with microstructure can be obtained. mold, and its height was measured to be about 20 μm.

[0075] Step 2: Pre...

Embodiment 2

[0081] Repeat Example 1 with the same steps described above, the difference is that in step 1, AZ-50 is used as the photoresist as AZ50, the mask plate is set according to the concave part of the PDMS stamp, and PDMS is prepared using the corresponding preparation process of AZ-50 seal.

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Abstract

The invention discloses a preparation method of a microarray chip. The method comprises the steps of target chip selection, auxiliary chip selection, chip docking, chip separation and the like, mother liquor droplets and buffer solution droplets of the surface of a target chip and the surface of an auxiliary chip are manipulated to be fused and split, the components in corresponding droplets are changed, and the obtained target chip is the microarray chip, target droplets are adsorbed on the hydrophilic region of the microarray chip, and the target droplets serve as a droplet array composed of multiple droplets with different components. According to the preparation method of the microarray chip, multiple droplets can be manipulated simultaneously to conduct fusion and separation, the droplet microarrays of concentration gradient, mechanical gradient, multicomponent chemistry, cell density, anisotropic gel and the like are formed, and broad prospects of preparation aspects on biological analysis and gel droplets are achieved.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and more specifically relates to a method for preparing a microarray chip and a product thereof. Background technique [0002] In recent years, microfluidic systems have had a significant impact in the fields of chemical engineering, bioengineering, and translational medicine. This microfluidic system combines materials science, chemical engineering, micro-electromechanical processing and other technologies to perform a series of operations such as sample injection, pretreatment, sample addition, sampling, reaction, and detection. Through the establishment of a large-scale integrated operating system, the analysis and screening throughput is higher, simpler and more effective. Because it is operated at the micron scale, it can also save sample costs and improve the accuracy of each unit experiment. The development of microfluidic systems will bring about a significant reduction in sc...

Claims

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Application Information

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IPC IPC(8): B01L3/00
Inventor 刘笔锋李一伟陈璞王亚超冯晓均
Owner 黑玉星岩国际科学技术(北京)有限公司
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