87results about How to "No need to mark" patented technology

The present invention provides a protein micro-array surface plasma resonance imaging detection system and its detection method. Said system includes surface plasma resonance micro-array protein sensor, incident arm, reflecting arm and signal processing unit, the described sensor includes prism, micro-array chip and sample cell, between prism and micro-array chip a refractivity oil layer is coated, the incident arm is positioned at one side of the sensor, successively includes semiconductor laser, collimator, polarizer, attenuator and reactangular light diaphragm, the reflecting arm is positioned at another side of the sensor, and successively includes lens and CCD receiver, and signal processing unit includes signal processing circuit and computer.

The invention discloses a multi-RCA (rolling circle amplification) method based on split padlock probes. Each novel split padlock probe is about 90bp in length and comprises four parts, namely a detection arm, a universal primer area, an HhaI endonuclease site and a tag sequence area, wherein an amplification system is composed of a connection system and an RCA system. The concrete detection method comprises the steps of: firstly, carrying out coupled reaction, mixing a target sequence DNA (deoxyribonucleic acid) segment and four split padlock probes of which the final concentration is 1mol / L, hybridizing for 15 minutes after boiling and degenerating, adding T4DNA ligase and T4DNA ligase buffer solution, supplying 10 muL of reaction system; carrying out exonuclease I and exonuclease III exterior contact for 45 minutes at 37 DEG C, preparing an annular template and then carrying out RCA reaction; mixing, boiling and degenerating 10 muL of connection product and universal primer, respectively adding dNTP, phi29DNA polymerase, HhaI restriction enzyme and buffer solution to form 20 muL of reaction system, stewing for 60 minutes at 37 DEG C; finally detecting single-chain DNA product of RCA with a specific tag sequence, thus obtaining a corresponding test conclusion according to the result.

The invention discloses a method for detecting fungaltoxin through multiple signals. The method comprises the following steps: 1) combining fungaltoxin with aptamer: adding a to-be-detected sample after the reaction of the fungaltoxin aptamer and complementary sequence thereof, thereby acquiring a mixed solution A; 2) amplifying a digestion auxiliary signal: reacting the mixed solution A with restriction enzyme, thereby acquiring a mixed solution B; 3) preparing a guanine tetramer structure: reacting the mixed solution B with tail-end deoxynucleotide transferase and deoxyribonucleoside triphosphate and reacting with ligand molecules, thereby acquiring a mixed solution C; and 4) detecting and analyzing: performing catalytic oxidation reaction on the mixed solution C and different substrates, generating ultraviolet, fluorescent and chemiluminescent signals and calculating the content of fungaltoxin in the to-be-detected sample according to the relation of the response strength of all the signals and the fungaltoxin concentration. The method disclosed by the invention has the characteristics of quick and simple operation and treatment, short detection time, marker-free effect, low cost, high precision, high sensitivity, and the like.

The invention discloses a DNA methylation detection method based on SERS (surface-enhanced Raman spectroscopy) of a gold nanopore array and an application of the method. The method comprises the following steps: (1) the periodic gold nanopore array is prepared by an electron beam lithography system through electron beam evaporation coating to serve as an SERS enhancing substrate; (2) surface Ramanenhancing performance of the SERS enhancing substrate is inspected by a Raman beacon molecule Rhodamine 6G, and the enhancing substrate is applied to DNA methylation detection; (3) DNA methylation detection based on a surface Raman enhancing effect is established. According to the detection method, the surface Raman enhancing performance of the prepared gold nanopore array is evaluated by the Raman beacon molecule Rhodamine 6G, and it is discovered that the gold nanopore array has good Raman enhancing effect on Rhodamine 6G and has efficient repeatability; the gold nanopore array is applied to DNA methylation detection. The method has the advantages of being high in sensitivity, simple to operate, high in repeatability and capable of being used for detecting DNA methylation.

The invention relates to a method for determining surfactant critical micelle concentration (CMC) based on the terahertz time-domain spectroscopy technology. The method comprises the steps that 1, a sample pool suitable for terahertz spectrum detection is prepared; 2, surfactant solution samples with different concentrations crossing four orders of magnitudes from 0.1 mM to 100 mM are prepared; 3, terahertz time-domain spectrum of a sample solution to be detected is measured and obtained, a relation spectrogram between the concentrations of the samples and terahertz absorption coefficients or refractive indexes are calculated and obtained through a formula, and the concentration, corresponding to a turning point of the spectrogram, of the surfactant sample is determined; 4, surfactant water solution samples which have a series of concentrations with smaller intervals are prepared according to the concentrations selected close to the determined sample turning point concentration; 5, terahertz time-domain spectra of sample solutions to be detected are measured and obtained again; 6, relation spectrograms between the concentrations of the samples to be detected and terahertz absorption coefficients or refractive indexes are calculated and obtained again, and the CMC of a surfactant is determined through spectrogram information. The method has the advantages of being rapid, free of marks, easy to operate, wide in application range, good in repeatability, high in result accuracy and the like.

The present invention provides a micro array protein chip, including optical glass base-plate, chromic film micro array, bare gold film micro array, coupling layer and acceptor, wherein the mercapto end connects with the bare gold film array, the carboxyl end connects with the acceptor. The method for making chips comprises, mask photo-etching of grating, making chromic film or gold film through deposition or sputtering, removing gridding and assembling coupling layer, mounting the acceptor on the coupling layer. The method by the invention can realize the protein chips of various amount units in an effective and low cost way.

The invention relates to a dialysis device and silicon nanowire field-effect tube combined biosensor. The dialysis device and silicon nanowire field-effect tube combined biosensor comprises a dialysisdevice, a silicon nanowire field-effect tube and a signal output computer, wherein the dialysis device is communicated with the silicon nanowire field-effect tube through a thin silica gel flexible pipe; and the silicon nanowire field-effect tube transmits a signal to the signal output computer through a probe. The serum desalting dialysis device and the silicon nanowire field-effect tube are connected into the same pipeline; the dialysis device is used as a filter; the field-effect tube having a double gate structure with a semiconductor silicon nanowire as a channel is used as a signal converter; an antibody corresponding to a tumor marker is used as a biological sensitive element; and therefore, the immediate, label-free and high-sensitivity detection of the serum tumor marker is realized.

The invention discloses a method for screening an aptamer via a high performance liquid chromatography, the aptamer and an application. The method for screening aptamers via high performance liquid chromatography comprises the following steps: screening a nucleic acid library of aptamers combined with docetaxel specificity from a random library RS36; screening the nucleic acid library by the high performance liquid chromatography to obtain the aptamer. In the high performance liquid chromatography, the stationary phase is an octadecyl bonded silica gel chromatographic column, the moving phase adopts water-acetonitrile gradient elution, the detection wavelength is 260nm and the detector is a diode array detector. The aptamer screened according to the high performance liquid chromatography has the advantages of being bonded with high specificity and high appetency of docetaxel, being free of immunogenicity, being capable of carrying out chemical synthesis and being good in biocompatibility, small in molecular weight, stable and easy to store, and can be applied to drug carriers, drug separation and purification, and preparation of docetaxel detection probes or target probes.

Provided are a thyroid gland neck tissue classifying and identifying system and method based on an autofluorescence technology and an optical coherence tomography. The system organically integrates anautofluorescence system and an optical coherence tomography system, utilizes high-sensitivity tissue element resolution capability of the autofluorescence system and high-resolution tissue structureimaging of the optical coherence tomography system to achieve quick, identifier-free, nondestructive, high-sensitivity and real-time thyroid gland neck tissue classification and identification. By adopting the thyroid gland neck tissue classifying and identifying system and method, the classification and identification efficiency and accuracy of doctors to parathyroid glands, thyroid glands, fat,lymph glands and other neck tissues in clinic thyroid gland operation can be effectively improved, the doctors can be assisted to protect the parathyroid glands and thoroughly remove the lymph glands.

The invention discloses a detection method and a detection test strip of salmonella enteritidis. Based on a Gram staining principle, crystal violet is used for staining bacteria into bluish violet firstly; then under the action of capillary effect, the stained bacteria are transferred along a nitrocellulose membrane; based on an antigen and antibody specific binding principle, the bacteria are captured by an antibody of a T line so that the T line becomes a visible strong bluish violet color. The bright bluish violet color can be obtained only if the bacteria are simply stained, and any nano-material labeled antibody is not used so that a complicated labeling process is omitted. According to the detection method and the detection test strip of the salmonella enteritidis, only one antibodyis scratched on the nitrocellulose membrane to detect directly and a traditional sandwich detection method adopting two antibodies is broken through, so that the cost is greatly saved and the difficulty of matching the antibodies is solved; the detection method and the detection test strip are simpler, more convenient and more novel.

The invention provides a biomolecule detection method and device. The biomolecule detection method comprises the steps that liquid to be detected is added to a nano sensor in covalent linkage with a probe molecule, and the probe molecule can generate specific binding with a biomolecule; under the condition of applying fixed bias on the nano sensor added with the liquid to be detected, first electrical characteristic data of the nano sensor of the liquid to be detected is measured; and comparing the first electrical characteristic data with second electrical characteristic data, whether the biomolecule exists in the liquid to be detected or not is determined, and the second electrical characteristic data is obtained by preloading liquid containing set concentration of biomolecule to the nano sensor covalently linked with the probe molecule and measuring the nano sensor at the fixed bias. The content of the biomolecule is detected through the biomolecule detection method, a target molecule can be prevented from being marked in the detection process, rapid and real-time detection can be realized, and whether the biomolecule is contained or not is determined or the concentration content is measured.

The invention discloses a new ratio-type label-free fluorescence biosensor for ultra sensitive high-specificity detection of lead ions (Pb2+), which is characterized in that: the constructed fluorescent biosensor is based on fluorescent silver nanoclusters with dual issue synthesized from DNA as a template; a fluorescent probe has the significant advantages of simple synthesis method, good stability and low cost; two character emission peaks are displayed at 560nm (green) and 685nm (red); and the existence of Pb2+ can cause fluorescence at 560nm to enhance and fluorescence at 585nm to decrease, the fluorescence intensity ratio between which (F560 / F685) shows good linear relationship with Pb2+ at the concentration range from 0.001nM to 10nM, has excellent selectivity to Pb2+, and has no obvious responses to copper, zinc, manganese, calcium, cadmium, iron, magnesium, potassium, nickel, chromium and other metal ions. The method provided by the invention can meet the requirement of on-site detection of being fast and accurate, and can be further applied to the development of relevant kits and test notes.

The invention belongs to the field of analysis and detection, and relates to a transcription factor fluorescence detection of based on DNA-silver nanocluster allosteric probe. The method includes a) using hairpin DNA as template, adding silver nitrate and sodium borohydride, and reducing that silver nitrate and sodium borohydride to generate DNA-silver nanocluster allosteric probe AgSwitch in situ; B) incubating a plurality of transcription factor solutions of different concentrations with AgSwitch obtained in step a); C) measuring the fluorescence value of the system, obtaining a linear relationship between the concentration of the transcription factor and the fluorescence intensity, and drawing a standard curve; D) measuring fluorescence intensity of the sample to be tested under the same conditions as the step c), and obtaining the concentration of transcription factors in the sample to be tested according to the standard curve of the step c). The method has the advantages of label-free, enzyme-free and high sensitivity, and can realize efficient detection of the transcription factors in the biological sample.

The invention provides a plasma nano platform for visual detection and application of the plasma nano platform, and belongs to the field of base mutation detection. The plasma nano platform is prepared from the following components: a hairpin type nucleic acid probe H2 functionalized-nanofiber membrane, a hairpin type nucleic acid probe H1 solution, an MES buffer solution with H2O2 concentration not less than 120 [mu]mol / L, a hemin solution at the concentration between 1 [mu]mol / L and 6 [mu]mol / L, an MES buffer solution containing HAuCl4 at the concentration between 0.2 mmol / L and 0.4 mmol / L,and a glutathione solution at the concentration between 100 [mu]mol / L and 120 [mu]mol / L. The plasma nano platform has the characteristics of simplicity, no marks, supersensitivity and repeated use. The plasma nano platform can detect a targeted DNA at an ultralow concentration in a human serum sample with naked eyes and can increase a concentration distinguishing ratio of a single base to a complete complementary sequence by 0.001 percent, so that the reliability of single base mispairing recognition is guaranteed.

The invention discloses a composite nano-probe based on N-Ti<3>C<2>QDs and o-phenylenediamine oxide, and discloses a ratio fluorescence detection method of the probe for H<2>O<2> and xanthine. The probe is the composite nano-probe, which is formed by bonding N-Ti<3>C<2>QDs to DAP through pi-pi conjugation and hydrogen bonds and used for ratio fluorescence detection of H<2>O<2> or xanthine. According to the invention, a dual-emission reverse ratio fluorescent sensing platform of H<2>O<2> based on photo-induced electron transfer (PET) is established by using quantum dots synthesized by a MXene two-dimensional material for the first time; furthermore, on the basis of the effect of xanthine generating H<2>O<2> under specific catalysis of xanthine oxidase, ratio fluorescence detection of xanthine is realized; and the detection method in the invention has the advantages of being free from labelling, high in sensitivity, high in accuracy and low in cost detection, and has good application prospect.

The invention discloses a fluorescence chemical method for detecting ALP (Alkaline Phosphatase) and application thereof. The fluorescence chemical method comprises the following steps of: firstly, designing a single-chain DNA (Deoxyribonucleic Acid) of which a 3' terminal is phosphorylated as a DNA probe, dephosphorylating the 3' terminal of the DNA probe and exposing a 3'-OH terminal when the ALPexists; secondly, performing catalytic addition on dTTPs (Deoxythymidine Triphosphates) at a 3'-OH terminal of the DNA probe by TdT (Terminal Deoxynucleotidyl Transferase), thus extending to generatea poly-T tail ssDNA, forming a dual-chain DNA mediated by T-HgII-T structure by the poly-T tail ssDNA and Hg ions, and drawing a detecting curve according to a detected fluorescence intensity signaland the concentration of the ALP after adding nucleic acid dye; finally, replacing the ALP with a to-be-detected sample, repeating the steps, and comparing a detecting result with the detecting curve,thus calculating the content of the ALP in the sample. The fluorescence chemical method disclosed by the invention is simple and convenient, is high in sensitivity and can be used for detecting the ALP or screening ALP inhibitors.

The invention relates to a rapid detection method for metal ions in wine. The method comprises the following steps: (1) a Schiff base metal ion probe is prepared by a Schiff base reaction; (2) gold nanorods (Au) are synthesized through seed growth; (3) the metal irons and the Schiff base are subjected to complexing reaction, and a Schiff base metal complex is formed; and (4) the generated complexis detected by using a portable Raman spectrometer, an ultraviolet spectrophotometer and a fluorescence spectrophotometer, and thus, qualitative and quantitative analysis and detection on Cu<2+> and Zn<2+> contained in a wine product are thus carried out. In comparison with the prior art, the Schiff base metal complex is detected by using SERS, ultraviolet and fluorescence sensors, the detection is more accurate, labeling and separation and purification do not needed, monitoring and detection on Cu<2+> and Zn<2+> in a wine product during the production and storage process of the wine can be realized, and the detection limit is 1*10<-10> M.

The invention discloses a sensor with a gold nanoparticle and quantum dot composite structure, a system and a method. Based on the local surface plasma resonance characteristics of the gold nanoparticles under total internal reflection, the microfluid technology is combined, the gold nanoparticles are directly combined with the transparent substrate, the binding force is strong, the thickness is accurate and controllable, the continuity is good, no pollution is caused, in addition, the optical property of the gold film is excellent, and the sensitivity and the accuracy are very good. Quantum dots are inserted into gaps among gold nanoparticles to obtain a gold nanoparticle quantum dot composite structure, the surface plasma resonance effect of the gold nanoparticles is enhanced, the sensoris more sensitive to changes of parameters such as the refractive index and the thickness of the composite structure and media nearby the composite structure, and research substances attached to thesurface of the structure can be detected more easily. The sensor has the advantages of high sensitivity, high stability, no marking, no damage, high accuracy, real-time rapid detection, wide application range and the like; the sensor has a great development prospect and a wide potential application value.

The invention discloses a polymer film conformation transition temperature detection method and system. The method comprises the following steps: measuring a terahertz time-domain spectral signal of atemperature control sample pool; selecting a temperature point according to a first preset interval in the temperature rise range of the temperature control sample pool to measure a first terahertz time-domain spectral signal of the thin film sample; determining the turning temperature of the film sample according to the terahertz time-domain spectral signal of the temperature control sample pooland the first terahertz time-domain spectral signal; selecting a temperature point according to a second preset interval within a preset temperature range of the transition temperature to measure a second terahertz time-domain spectral signal of the thin film sample; and determining the conformational transformation temperature of the film sample according to the terahertz time-domain spectral signal of the temperature control sample cell and the second terahertz time-domain spectral signal. The terahertz time-domain spectroscopy technology is used for detecting the conformational transformation temperature of the polymer film, so that the method has the advantages of being rapid, free of marks, easy to operate, wide in application range, good in repeatability, high in result accuracy andthe like.

The invention relates to a DNA sensor based on a 3 (2, 2'-bipyridyls) ruthenium solid electrochemiluminescence, a preparation method and applications used for the DNA sensor, belonging to the technical field of analytical chemistry and chemical sensors. The DNA electrochemiluminescence sensor is prepared by the modified glass carbon electrode of a nanometer pipe / a perfluorosulfonic acid ion-exchange membrane / 3(2, 2'-bipyridyls) ruthenium and the catalytic oxidation reaction of guanine base and adenine base conducted on the basis of 3(2, 2'-bipyridyls) ruthenium; the DNA sensor can be used for the electrochemistry and electrochemiluminescence double detection of the DNA, and can be also used for the electrochemistry and electrochemiluminescence double detection of thermally denaturated DNA; and furthermore, the DNA sensor can be also used for the electrochemistry and electrochemiluminescence detection of DNA single base mismatch. The DNA sensor has high sensitiveness and simple operation, and needs no marks during the detection process; and the detection of single base mismatch has universality and can be used for the detection of DNA fragments with any sequences.

The invention relates to a preparation method of a graphene biosensor for specific protein detection. Graphene in the specific protein biosensor is prepared by a high-temperature annealing method in an inert atmosphere. A functional graphene film is designed and prepared, and the graphene specific protein biosensor is also manufactured. Tests show that the specific protein biosensor can perform real-time unmarked detection on specific protein and allows influences caused by concentration changes of target protein to be seen clearly. A graphene biochip has a bright application prospect in the aspects of biomolecule sensors and biomolecule detection.

The invention discloses a method for simultaneously detecting two types of HIV DNA by using silver nano-clusters (AgNCs) as a label-free fluorescence biosensing platform. The fluorescence biosensing platform is based on two features of the silver nano-clusters: (1) fluorescence of the silver clusters can be enhanced in multiples by a rich guanine (G) sequence; and (2) the fluorescence can be remarkable enhanced by mutual extrusion of two neighboring AgNCs. Different templates of the synthetic AgNCs are designed at two ends of a single chain, and two different signals with different luminous peaks are obtained so as to avoid signal interference. A DNA chain mispaired with the single chain is designed as a template of which one end is a rich-G sequence, and the other end is a synthetic silver cluster extrusion pair. A target object is completely paired with complementary DNA, high fluorescence of the AgNCs is damaged, and a fluorescence signal is weakened. Only one type of the color-variable silver nano-clusters needs to be used as a fluorescence probe so that two wider detection ranges (0.2-700 nm) can be obtained. A limit of detection is as low as 0.2 nm, and high sensitivity detection of multiple types of DNA or MiRNA can be further realized, so accuracy and practicability of bioanalysis are improved.

The invention discloses a three-dimensional culture human myocardial cell pulsation detection method based on image edge extraction. The three-dimensional culture human myocardial cell pulsation detection method specifically comprises the following steps: taking a first frame of a myocardial cell pulsation video sequence frame as a reference image; converting the reference image into a grayscale image; obtaining a sub-image of each myocardial cell cluster; extracting coordinates and gray values of edge position points of each myocardial cell group, and calculating a gray average value of reference edge points of each myocardial cell group; taking a sequence frame of a subsequently acquired myocardial cell pulsation video as a real-time image, and carrying out parallel graying processing; according to the coordinate positions of the reference edge points in the reference image, calculating a gray average value of the reference edge points of each myocardial cell group in the real-time image; drawing a myocardial cell pulsation curve of each myocardial cell cluster; and detecting the cardiac muscle cell pulsation frequency and amplitude in the cardiac muscle cell pulsation curve. Thecardiac muscle cell pulsation characteristics are automatically detected based on the image edge extraction technology, and the three-dimensional culture human myocardial cell pulsation detection method has the advantages of being intelligent, non-invasive, low in cost, repeatable and real-time in processing.

The invention discloses a difunctional G-quadruplex allosteric biosensor for detecting beta-lactoglobulin. The difunctional G-quadruplex allosteric biosensor is prepared by the following steps: (1) designing a difunctional G-quadruplex aptamer of beta-lactoglobulin; (2) optimizing the reaction conditions of the beta-lactoglobulin biosensor; and (3) detecting the beta-lactoglobulin. According to the invention, the design principle is that the beta-lactoglobulin biosensor sequence is subjected to conformational change before and after a detection target is introduced, the catalase activity of G-quadruplex / hemin DNA bionic enzyme is influenced, and then beta-lactoglobulin detection is realized; and the G-quadruplex sequence responds to the target concentration, such that the solution presents the corresponding colorimetric gradient after 3, 3', 5, 5'-tetramethyl benzidine (TMB) color development is catalyzed so as to finally achieve the simple, rapid, low-cost and ultra-sensitive colorimetric detection of the beta-lactoglobulin.

The invention discloses an anti-AChE interference method capable of quickly detecting BChE activity. The method comprises the following steps of: 1) with sulfonated calix[4]arene and dye lucigenin as a sensing pair for enzyme activity detection and succinylcholine as an enzyme reaction substrate, preparing a solution containing 0.5(mu)mol / L SC4A, 0.5(mu)mol / L LCG and 10(mu)mol / L SuCh by use of 10mmol / L phosphoric acid buffer solution with pH being 8.0 at 37 DEG C, and establishing a supermolecule serial detection system; and 2) adding 1U / ml BChE into the system to obtain an obvious fluorescence quenching signal within 15 minutes. The method disclosed by the invention has the advantages of simple technology, convenience in operation and sensitivity and high speed, can detect the activity of BChE with high selectivity in the presence of excessive AChE, and has broad application prospect in the specific detection of BChE activity and treatment field of Alzheimer's disease.

The invention discloses a Mach-Zehnder interference optical fiber based on a surface plasma effect and a sensor thereof, which belong to the field of optical fiber sensors. According to the Mach-Zehnder interference optical fiber based on the surface plasma effect, a first layer of metal film is arranged on the periphery of a sensing area optical fiber, a polymer layer is arranged on the peripheryof the first layer of metal film, and a second layer of metal film is arranged on the periphery of the polymer layer; the thickness of the first layer of metal film is 20 to 200nm, the thickness of the polymer layer is 150 to 180nm, and the thickness of the second layer of metal film is 20 to 50nm. According to the optical fiber, a heavy metal film can generate surface plasma waves under the action of light waves, and two beams of plasma waves can be generated in a double-layer heavy metal film structure, so that Mach-Zehnder interference can be formed. Change of an external environment to bedetected can cause movement of an interference spectrum, and high-sensitivity detection is achieved according to the movement amount of the wavelength. The structure is simple, the refractive index sensitivity is high, and the sensor can work in any waveband.

The invention discloses a method for detecting the killing effect of gamma-delta T cells to tumor cells by utilizing a real-time cell analysis system and belongs to the technical field of biological medicines. The method is characterized by using the Real-Time Cell Analysis system (RTCA) to detect the killing effect of the gamma-delta T cells to the tumor cells. The method disclosed by the invention has the advantages of high sensitivity, good stability and capability of detecting the killing effect of the gamma-delta T cells to the tumor cells continuously and dynamically in real time without marks.

The invention discloses a method for detecting the content of acetylcholinesterase in human serum by using a boron nitride quantum dot-gold nanoparticle nanocomposite. The method comprises the following steps: 1) establishing a standard curve of fluorescence intensities and acetylcholinesterase concentrations: A, preparing iodinated acetylcholine-boron nitride quantum dot solutions; B, respectively adding acetylcholinesterase solutions with the same volumes and different concentrations into the iodinated acetylcholine-boron nitride quantum dot solutions, then adding HAuCl4, and carrying out uniform mixing; and C, after reaction of a series of mixed solutions, respectively determining the fluorescence intensities of the solutions, and drawing a standard curve of the fluorescence intensitiesand the acetylcholinesterase concentrations so as to obtain a linear regression equation; and 2) preparing a mixed solution from a to-be-measured serum sample according to the method prescribed in the sub-step B, determining the fluorescence intensity of the mixed solution, substituting the fluorescence intensity into the linear regression equation to obtain an acetylcholinesterase concentration.The method can be applied to the actual detection of human serum samples, and has the characteristics of simplicity, rapidness and sensitivity.