Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DNA sensor based on three (2,2'-bipyridyl) ruthenium solid-state electrochemistry illumination, and production method and use thereof

A bipyridine, electrochemical technology, applied in the field of analytical chemistry and chemical sensors, to achieve the effect of simple operation and high sensitivity

Inactive Publication Date: 2009-04-08
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the electrochemiluminescence detection of single base mismatches in DNA has been rarely reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA sensor based on three (2,2'-bipyridyl) ruthenium solid-state electrochemistry illumination, and production method and use thereof
  • DNA sensor based on three (2,2'-bipyridyl) ruthenium solid-state electrochemistry illumination, and production method and use thereof
  • DNA sensor based on three (2,2'-bipyridyl) ruthenium solid-state electrochemistry illumination, and production method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of tris(2,2'-bipyridine) ruthenium solid-state electrochemiluminescence DNA sensor

[0039] (1) Grind the glassy carbon electrode to make its surface smooth and ultrasonic, wash the electrode surface with water to obtain a pretreated glassy carbon electrode;

[0040] (2) Pour 10μL of 0.5mg / mL "carbon nanotube / perfluorosulfonic acid ion exchange membrane" mixed liquid on the surface of the glassy carbon electrode pretreated in ①, and dry;

[0041] (3) The glassy carbon electrode obtained in (2) is immersed in a solution of 1 mM tris(2,2'-bipyridine) ruthenium, and the tris(2,2'-bipyridine) ruthenium is fixed by ion exchange to prepare "carbon nano Tube / perfluorosulfonic acid ion exchange membrane / Tris(2,2'-bipyridine) ruthenium" modified glassy carbon electrode (denoted as RuGC electrode);

[0042] (4) Wash the RuGC electrode obtained in (3) with a large amount of water to remove the physically adsorbed tris(2,2'-bipyridine) ruthenium, and obtain solid electroche...

Embodiment 2

[0044] Using tris(2,2'-bipyridine) ruthenium solid-state electrochemiluminescence DNA sensor to measure salmon sperm double-stranded DNA

[0045] (1) Add 5μL 3.04×10 -8 The mol / L salmon double-stranded DNA sample is dropped on the electrode surface of the sensor and dried;

[0046] (2) Next, use this sensor containing salmon sperm double-stranded DNA as a working electrode, immerse it in an electrochemical cell containing 2 mL of pH 5.5 10 mM acetate buffer solution (containing 50 mM NaCl) for electrochemical and electrochemiluminescence measurements.

Embodiment 3

[0048] Using tris(2,2’-bipyridine) ruthenium solid-state electrochemiluminescence DNA sensor to measure heat-denatured salmon sperm double-stranded DNA

[0049] (1) Put 3.04×10 -8 The mol / L salmon sperm double-stranded DNA is boiled in a water bath for 5 minutes, and then immediately cooled in an ice bath to obtain thermally denatured salmon sperm double-stranded DNA;

[0050] (2) Drop 5μL of heat-denatured salmon sperm double-stranded DNA obtained in (1) on the electrode surface of the sensor, and dry;

[0051] (3) Next, use this sensor containing thermally denatured salmon sperm double-stranded DNA as a working electrode, immerse it in an electrochemical cell containing 2 mL of 10 mM acetate buffer solution (containing 50 mM NaCl) with a pH of 5.5 for electrochemical and electrochemiluminescence measurements .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a DNA sensor based on a 3 (2, 2'-bipyridyls) ruthenium solid electrochemiluminescence, a preparation method and applications used for the DNA sensor, belonging to the technical field of analytical chemistry and chemical sensors. The DNA electrochemiluminescence sensor is prepared by the modified glass carbon electrode of a nanometer pipe / a perfluorosulfonic acid ion-exchange membrane / 3(2, 2'-bipyridyls) ruthenium and the catalytic oxidation reaction of guanine base and adenine base conducted on the basis of 3(2, 2'-bipyridyls) ruthenium; the DNA sensor can be used for the electrochemistry and electrochemiluminescence double detection of the DNA, and can be also used for the electrochemistry and electrochemiluminescence double detection of thermally denaturated DNA; and furthermore, the DNA sensor can be also used for the electrochemistry and electrochemiluminescence detection of DNA single base mismatch. The DNA sensor has high sensitiveness and simple operation, and needs no marks during the detection process; and the detection of single base mismatch has universality and can be used for the detection of DNA fragments with any sequences.

Description

Technical field [0001] The invention relates to a DNA sensor based on tris(2,2'-bipyridine) ruthenium solid-state electrochemiluminescence, a preparation method and application thereof, and belongs to the technical field of analytical chemistry and chemical sensors. Background technique [0002] DNA is a carrier of biological genetic information, and its analysis and detection have great scientific value and practical significance in the fields of DNA sequencing, DNA expression profile analysis, clinical analysis, and mutation analysis of disease-related genes. In order to meet the needs of DNA analysis and testing in these fields, researchers have developed many DNA testing methods. These methods include: fluorescence, mass spectrometry, electrochemistry, etc. [0003] The electrochemiluminescence of tris(2,2'-bipyridine) ruthenium has the advantages of high sensitivity, good selectivity, and wide measurement linear range, so it has been standardized and applied in fields such a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76C12Q1/68
Inventor 魏辉杜艳康建珍汪尔康
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com