The invention relates to a whole
genome combined targeted amplification
library building method, a
reagent and a
pathogen detection method. The
library building method comprises the following steps: obtaining a
linker connection product of which two ends are connected with double linkers after whole
genome DNA fragmentation of a sample, wherein the
linker connection product comprises an optional target area and a non-target area; taking the
linker connection product as a template, adopting a first
gene specific primer and a downstream
library amplification universal primer to carry out first PCR amplification to obtain a first amplification product, combining the first
gene specific primer with the target region, and combining the downstream library amplification universal primer with a first chain of the double linker; and taking the first amplification product as a template, performing second PCR amplification by adopting a second
gene specific primer, a downstream library amplification universal primer and an upstream library amplification universal primer to obtain a second amplification product, combining the second
gene specific primer with the target region, and combining the upstream library amplification universal primer with a second chain of the double-chain connector. The method enriches the target area, does not affect the non-target area, and is low in detection cost and high in efficiency.