71results about How to "Detection does not affect" patented technology

The invention discloses a time difference type ultrasonic flowmeter measuring method based on zero point analysis. The time difference type ultrasonic flowmeter measuring method based on zero point analysis is a method for accurately determining fair-current transmission time and countercurrent transmission time through a zero point analysis method. The time difference type ultrasonic flowmeter measuring method based on zero point analysis comprises the steps that (1) after a pulse drive signal is sent out, an A/D converter is started to conduct data collection; (2) a zero point value is searched; (3) whether a zero point is an ultrasonic signal zero crossing point or not is judged; (4) a first zero crossing point in an ultrasonic receiving signal is selected so that the ultrasonic signal arriving time can be determined and the fair-current transmission time and the countercurrent transmission time can be calculated; (5) instant flow of a section to be measured is calculated through the fair-current transmission time and the countercurrent transmission time. The zero point of the ultrasonic signal is not influenced by the external environment, the zero point can be detected even under the conditions that the signal is weak, fluctuation is large and interference is strong, the fair-current transmission time and the countercurrent transmission time of the ultrasonic signal in fluid can be accurately measured, the measuring accuracy of a time difference type ultrasonic flowmeter is greatly improved, and the problems brought by an existing threshold value comparing method are thoroughly solved.

The invention discloses a multi-RCA (rolling circle amplification) method based on split padlock probes. Each novel split padlock probe is about 90bp in length and comprises four parts, namely a detection arm, a universal primer area, an HhaI endonuclease site and a tag sequence area, wherein an amplification system is composed of a connection system and an RCA system. The concrete detection method comprises the steps of: firstly, carrying out coupled reaction, mixing a target sequence DNA (deoxyribonucleic acid) segment and four split padlock probes of which the final concentration is 1mol/L, hybridizing for 15 minutes after boiling and degenerating, adding T4DNA ligase and T4DNA ligase buffer solution, supplying 10 muL of reaction system; carrying out exonuclease I and exonuclease III exterior contact for 45 minutes at 37 DEG C, preparing an annular template and then carrying out RCA reaction; mixing, boiling and degenerating 10 muL of connection product and universal primer, respectively adding dNTP, phi29DNA polymerase, HhaI restriction enzyme and buffer solution to form 20 muL of reaction system, stewing for 60 minutes at 37 DEG C; finally detecting single-chain DNA product of RCA with a specific tag sequence, thus obtaining a corresponding test conclusion according to the result.

Fundamental principle of the invention is that multiple fonts are designed for same character (string) in same semantic through changing relation of connection and disconnection between strokes of constituting character (string). Moreover, proper encoding is carried out for topological structure of these character fonts, and digital water ink information is carried out by these codes for different fonts of characters (string). The invention includes related contents: (1) method for designing multiple fonts of character; (2) method for encoding the said fonts; (3) method of unified encoding; (4) text digital water ink technique for encoding fonts respectively; (5) unified text digital water ink technique for encoding fonts. Features are: small influence on vision, simple and reliable detection method, good expansibility and large capability of information.

The invention provides lateral chromatographic test paper for chemical detection. The test paper is assembled according to a lateral chromatographic structure based on a reaction sequence of the chemical detection. The test paper comprises a detection reaction condition preparation area, a detection color development area and a water absorption area which are successively arranged on a bottom plate, wherein the detection reaction condition preparation area is qualitative filter paper, quantitative filter paper, glass cellulose membrane, water absorption paper or polyester cellulose membrane test paper loaded with a reaction reagent, and the detection color development area is qualitative filter paper, quantitative filter paper, glass cellulose membrane, water absorption paper or polyester cellulose membrane. The test paper is uniform in color development, capable of solving the problem in the existing detection method that various reaction reagents cannot coexist on a piece of paper and breaking the limitation of the chemical detection test paper, and is more reliable in detection result.

The invention relates to a protein suspension chip of ricin and methods for preparing, quantifying and detecting the same. The detecting method comprises the following steps: adding a working solution and a sample to be tested into a hole; adding a detection antibody; adding SA-PE; adding a detection buffer solution and mixing the mixture evenly; and reading numerical values, analyzing data and judging the detection result. The quantifying method comprises the following steps: adding a positive test sample or a standard product for dilution through gradient multiple proportions, detecting a series of diluted samples in a suspension chip system, and reading a corresponding fluorescence value (MFI); making a dosage-reaction curve; and fitting the dosage-reaction curve and an equation. The suspension chip comprises a coded microsphere, a ricin capturing antibody coated on the microsphere, a biotin-marked ricin detection antibody, a streptavidin-phycoerythrin and the buffer solution. The methods have the advantages of good detectability, high sensitivity, strong specificity and wide dynamic range.

The invention discloses a method for electrochemical detection of lead ions of an ordered silicon nano-pore film/indium tin oxide electrode. The method comprises the following steps: (1) mixing the sample to be tested with the buffer solution to obtain liquid to be tested with pH of 3.6 to 6; (2) using the ordered silicon nano-pore film/indium tin oxide electrode as the working electrode, gathering the lead ions in the liquid to be tested by the constant voltage method with the gathering voltage of 0.8-0. 6V; (3) placing the gathered electrode in the buffer solution of pH value at 3.6-6.0, measuring the peak current by means of the differential pulse voltammetry method, and according to the standard curve, calculating the concentration of the lead ions in the sample to be tested, wherein the starting potential of the differential pulse voltammetry is 0.95-0.65V and the termination potential is 0.4-0.5V. The method of the invention can realize the detection of trace lead ions, and has potential application value in the detection of the actual sample, especially the detection of the biological complex sample.

The invention relates to the technical field of chemiluminiscence immunoassay, in particular to a homogeneous immunoassay method for quenching acridinium ester chemiluminiscence based on an ortho-position touch effect and acridinium ester chemiluminiscence and use equipment. A detection solution adopted in the analysis method comprises a DNA1-conjugate, a DNA2-conjugate, acridinium ester (AE) labeled DNA3, a graphene oxide (GO) combined antioxidant (AOD) and the like. The method is a homogeneous chemiluminiscence immunoassay method, separation and cleaning steps are not needed, the operation is simple, meanwhile, the turnover time (TAT) of clinical examination specimens is greatly shortened, centrifugal treatment is not needed for blood specimens, whole blood loading can be achieved, and adetection report can be given within 5-10 minutes; the acridinium ester derivative (AE) is different from fluorescein (Cy5 and the like) substances and can realize anti-interference chemiluminescencedetection; and the method not only can be used for detecting macromolecular protein and antibodies, but also can be used for realizing immunodetection of chemical small molecules.

The invention relates to a new quantitative detection method and a product for detecting tuberculosis antibody in a serum sample. The method of the invention has good detection capability, high sensitivity, strong specificity and wide dynamic range. The invention establishes an open detection modularised platform of pathogenic bacteria antibody protein suspension chip detection with the tuberculosis antibody as a representative.

The invention discloses a water quality detection device with a cleaning function. The device comprises a device box body, and a filtering cavity is formed in the left side in the device box body; filter screens are arranged between the left wall and the right wall of the filtering cavity in a bilateral symmetry and rotatable manner; a water pumping cavity is formed in the rear side of the filtering cavity; a detection cavity is formed in the right side of the filtering cavity; a lifting device is arranged on the upper side of the filtering cavity; a water tank cavity is formed in the upper side of the detection cavity in a front-back symmetrical and communicating manner; a water tank capable of moving up and down is arranged in the water tank cavity; and a telescopic device for controlling the water tanks on the front side and the rear side to synchronously move up and down is arranged on the upper side of the water tank cavity. According to the invention, sewage is subjected to solid-liquid separation, the sewage is detected, then the detection cavity is cleaned in an all-around mode in a water washing mode, meanwhile, the device can discharge garbage in time, and use of the nexttime is not affected.

The invention relates to a new method for quantitatively detecting SARS antibody of protein suspended chip in SARS antibody and a product thereof. The method of the invention has good detection ability, high flexibility, strong specificity and wide dynamic range, and establishes open detection modelization platform detected by virus antibody protein suspended chip using SARS antibody as representative.

The invention discloses a feeding and blanking device. The feeding and blanking device comprises a stock bin device, a first manipulator, and a second manipulator. The stock bin device is used for storing trays. The first manipulator is used for conveying the trays where to-be-detected products are placed to the stock bin device and moving the trays where the detected products are placed out of the stock bin device. The second manipulator is used for taking the to-be-detected products out of the trays of the stock bin device, placing the to-be-detected products on detection stations, then taking the detected products out of the detection stations and finally putting the detected products back into the empty trays of the stock bin device. The stock bin device comprises a first stock bin used for placing the trays which are conveyed by the first manipulator and loaded with the to-be-detected products, a second stock bin used for storing the empty trays conveyed by the first stock bin, and a third stock bin is used for receiving the empty trays conveyed by the second stock bin, and the detected products are put back into the original trays in the third stock bin. According to the feeding and blanking device, the trays can be properly placed, and the detected products can be put back into the original trays.

The invention discloses an air coupling ultrasonic detection method for vertical honeycomb spliced interfaces. The air coupling ultrasonic detection method comprises the following steps: a multi-layerhoneycomb structure is vertically spliced; a layer of detachable skin is laid on each of the upper surface and the lower surface of the obtained multi-layer honeycomb structure; first curing treatment is conducted on the multi-layer honeycomb structure paved with the detachable skin; air coupling ultrasonic detection is conducted on the multi-layer honeycomb structure subjected to primary curingtreatment, and whether all the honeycomb spliced interfaces are debonded or not is detected; if all honeycomb spliced interfaces do not have the debonding defect, the detachable skin on the upper surface and the lower surface of the multi-layer honeycomb structure is torn off; and surface skin is bonded to the upper surface and the lower surface of the multi-layer honeycomb structure, secondary curing is conducted on the multi-layer honeycomb structure bonded with the surface skin, and a vertically-spliced multi-layer honeycomb structure part is obtained. The air coupling ultrasonic detectionmethod for the vertical honeycomb spliced interfaces is high in detection accuracy, the quality of vertically-spliced multi-layer honeycomb sandwich structure part can be guaranteed, and safe and reliable product manufacturing is guaranteed.

The invention discloses a rapid melamine detection test paper strip. The test paper strip comprises a bottom plate, a sample pad, a filtering layer, a selenium mark combining pad, a reaction pad and a water absorption pad. The structure of a conventional melamine test paper strip is optimized and improved, namely, one section of a filtering membrane is additionally arranged between the sample pad and the selenium mark combining pad and is used as the filtering layer, so that the problems that certain components are not easy to dissolve when an existing test paper strip is used for detecting milk powder and solid particles or macromolecules block a membrane pore channel, the detection time is prolonged and detection results are not accurate are solved. When the test paper strip is used for detecting melamine, the detection accuracy can be improved and a false negative result is prevented from occurring; and the rapid melamine detection test paper strip can be widely applied to laboratories, families and field detection.

The invention relates to a new quantitative detection method and a product for detecting avian influenza antibody in a serum sample, and a preparation method thereof. The method of the invention has good detection capability, high sensitivity, strong specificity and wide dynamic range. The invention establishes an open detection modularised platform of pathogenic bacteria antibody protein suspension chip detection with the avian influenza as a representative.

The invention discloses a reverse torque detection device and reverse torque detection method for a one-way bearing. The device comprises a first fixture body, a second fixture body, a mandrel, four long bolts, and four nuts (5) matched with the long bolts, four short bolts and a fixing plate. According to the reverse torque detection device and reverse torque detection method for the one-way bearing of the invention, the first fixture body and the second fixture body are combined to fix the outer ring end surface of the one-way bearing; the mandrel and a ferrule are adopted to fix the inner ring end surface of the one-way bearing; since an end surface loading mode is adopted, the related drawbacks of the outer circle loading of a common detection device can be avoided; detected data obtained by the device of the invention are more realistic, stable and reliable; the fixing plate is used for fixing the mandrel when a hexagon bolt is tightened, and therefore, the inner ring of the bearing can be prevented from rotating with the mandrel when the inner ring of the bearing is clamped, so that a reverse torque applied to the inner ring of the bearing can be avoided indirectly; the fixing plate is removed before detection, so that the detection of the reverse torque of the reverse bearing is more realistic and reliable.

The invention discloses a preparation method and a using method of a protein suspension chip for detecting a tick borne encephalitis antibody in a serum sample. The using method of the invention has the advantages of high detection capacity, high flexibility, high specificity and wide dynamic range; and an open detection modularized platform for the detection of the protein suspension chip on a viral antibody represented by the tick borne encephalitis antibody is established.

The invention provides a convenient and economic combined gene chip which relates to the technical fields of the bio-chip technology and the in vitro clinical diagnosis and detection, and the combined gene chip is formed by combining a carrier and a sticky membrane which has a pierced reaction zone and covers on the carrier. The combined gene chip is used for conveniently and rapidly carrying out the multi-disease and multi-person parallel diagnosis, the requirements on the manufacturing process of the carrier of the gene chip are simultaneously reduced, the cost input is reduced, and the combined gene chip is conductive to the popularization and the application of the gene chip for the multi-indicator and multi-person parallel detection.

The invention relates to a whole genome combined targeted amplification library building method, a reagent and a pathogen detection method. The library building method comprises the following steps: obtaining a linker connection product of which two ends are connected with double linkers after whole genome DNA fragmentation of a sample, wherein the linker connection product comprises an optional target area and a non-target area; taking the linker connection product as a template, adopting a first gene specific primer and a downstream library amplification universal primer to carry out first PCR amplification to obtain a first amplification product, combining the first gene specific primer with the target region, and combining the downstream library amplification universal primer with a first chain of the double linker; and taking the first amplification product as a template, performing second PCR amplification by adopting a second gene specific primer, a downstream library amplification universal primer and an upstream library amplification universal primer to obtain a second amplification product, combining the second gene specific primer with the target region, and combining the upstream library amplification universal primer with a second chain of the double-chain connector. The method enriches the target area, does not affect the non-target area, and is low in detection cost and high in efficiency.