The invention discloses an MMLV
reverse transcriptase mutant, which belongs to the technical field of
biology and is obtained by
mutation design of specific sites of
wild type MMLV
reverse transcriptase. The
mutation site comprises one or a combination of more of A32, S56, E69, L139, T163, T197, H204, S232, N249, T306, F309, T330, G424, L435, D449, D524, E562, D583, L603, H642 and T664, and the
mutation site comprises one or a combination of more of the A32, the S56, the E69, the L139, the T163, the T197, the H204, the S232, the N249, the T306, the F309, the T330, the G424, the L435, the D449, the Compared with the existing MMLV
reverse transcriptase, the MMLV reverse transcription
mutant disclosed by the invention has higher reverse transcription temperature and rate. The invention also discloses a preparation method of the MMLV reverse transcriptase
mutant and application of the MMLV reverse transcriptase mutant in reverse transcription reaction.