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Rapid melamine detection test paper strip

A technology for detecting test strips and melamine, which can be used in testing food, dairy products, measuring devices, etc., to solve problems such as prolonged detection time, false negatives, blockage of membrane pores by solid particles or macromolecules, and to improve detection accuracy and accuracy. high degree of effect

Inactive Publication Date: 2015-09-30
杨同玥
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing test strips for detecting melamine do not have a filter layer. When detecting milk powder, because some components are not easy to dissolve, solid particles or macromolecules often block the membrane pores, which prolongs the detection time and is prone to false negative detection results.

Method used

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  • Rapid melamine detection test paper strip
  • Rapid melamine detection test paper strip
  • Rapid melamine detection test paper strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 4

[0017] The making of embodiment one melamine rapid detection test strip

[0018] The preparation of the test strip is based on the colloidal selenium immunochromatographic detection technology. Since melamine is a small molecule and has only one epitope, the preparation of the rapid detection test strip for melamine in this embodiment chooses the competitive method.

[0019] 1. Mouse-derived anti-melamine monoclonal antibody labeled with colloidal selenium particles

[0020] Colloidal selenium was added to the mouse-derived anti-melamine monoclonal antibody (available from Guangzhou Wowei Biotechnology Co., Ltd.) at a final concentration of 0.5-30 μg / mL, mixed gently for 10 minutes, and the colloidal selenium was observed by the transmission electron microscope. The degree of embedding of the source anti-melamine antibody can determine the optimal amount of labeling.

[0021] Draw 5ml of prepared colloidal selenium particles, and adjust the appropriate pH with potassium car...

Embodiment 5

[0032] The detection experiment of embodiment two melamine rapid detection test strips

[0033] 1. Performance testing experiment of melamine rapid detection test strip

[0034] Add different concentrations of melamine to commercially available milk powder that has been identified as being free of melamine, and detect the concentration of melamine in the sample through the melamine rapid detection test strip, thereby verifying the effectiveness of the melamine rapid detection test strip.

[0035] Detection experiment of melamine in commercially available milk powder: Add milk powder and 40mL distilled water into a 50mL centrifuge tube, mix well, and dilute step by step with the prepared 2mg / mL standard melamine solution to obtain melamine containing 0, 1, 5, 10, 50, 100 and 500μg respectively / L standard melamine sample for test strip detection.

[0036]Put the end of the test paper marked with the arrow pointing down, insert it into the sample milk powder solution, and obs...

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Abstract

The invention discloses a rapid melamine detection test paper strip. The test paper strip comprises a bottom plate, a sample pad, a filtering layer, a selenium mark combining pad, a reaction pad and a water absorption pad. The structure of a conventional melamine test paper strip is optimized and improved, namely, one section of a filtering membrane is additionally arranged between the sample pad and the selenium mark combining pad and is used as the filtering layer, so that the problems that certain components are not easy to dissolve when an existing test paper strip is used for detecting milk powder and solid particles or macromolecules block a membrane pore channel, the detection time is prolonged and detection results are not accurate are solved. When the test paper strip is used for detecting melamine, the detection accuracy can be improved and a false negative result is prevented from occurring; and the rapid melamine detection test paper strip can be widely applied to laboratories, families and field detection.

Description

technical field [0001] The invention belongs to a rapid detection test strip for detecting melamine in milk powder. The test strip adopts colloidal selenium immune chromatography detection technology and belongs to the field of food safety detection. Background technique [0002] Melamine (MEL), also known as melamine and cyanuric triamide, is an important nitrogen heterocyclic organic chemical raw material. Because the food industry often needs to determine the protein content in food, and because the technology of directly measuring protein is relatively complicated, the Kjeldahl method is commonly used to indirectly determine the protein content in food. Due to the high nitrogen content of melamine molecules, as long as it is added to food, it can cause the false impression of high protein content in the detection. At present, melamine residues are found in feed, milk, milk powder, biscuits, toffee and other related products. The results of animal experiments show that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/04G01N33/558
Inventor 不公告发明人
Owner 杨同玥
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