A method for detecting the content of acetylcholinesterase in human serum by using boron nitride quantum dot-gold nanoparticle nanocomposite

A technology of acetylcholinesterase and gold nanoparticles, which is applied in the field of analytical chemistry and nanometers, and can solve problems such as time-consuming and low sensitivity

Active Publication Date: 2020-09-01
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for the detection of acetylcholine in human serum by using boron nitride quantum dots-gold nanoparticles Enzyme Assay Method

Method used

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  • A method for detecting the content of acetylcholinesterase in human serum by using boron nitride quantum dot-gold nanoparticle nanocomposite
  • A method for detecting the content of acetylcholinesterase in human serum by using boron nitride quantum dot-gold nanoparticle nanocomposite
  • A method for detecting the content of acetylcholinesterase in human serum by using boron nitride quantum dot-gold nanoparticle nanocomposite

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Embodiment 1

[0031] A method for detecting the content of acetylcholinesterase in human serum by using a boron nitride quantum dot-gold nanoparticle nanocomposite, the specific steps are:

[0032] 1) Synthesis of ATCh-BNQDs

[0033] To 20 mL 0.2mg / mL BNQDs (for characterization see figure 1 ) solution was added 70mg ATCh, mixed and shaken at 37°C for 1h, the mixed solution was passed through a 1000Da dialysis bag to remove unbonded ATCh to obtain ATCh-BNQDs, and whether the product was successfully synthesized was characterized by ultraviolet-visible absorption spectrum, Zeta potential and infrared spectrum ,See figure 2 , and finally quantify the concentration of ATCh-BNQDs by UV-Vis absorption spectroscopy;

[0034] 2) AChE regulates the feasibility of growing AuNPs in situ

[0035] Add 50 μL of different concentrations of AChE to 100 μL of 0.1 mg / ml ATCh-BNQDs solution, followed by 50 μL of 0.8 mM HAuCl 4 Solution, after the mixed solution was reacted at 37°C for 32 minutes, the so...

Embodiment 2

[0045] BNQDs in response to different concentrations of AChE inhibitor dioxon regulated AuNP-induced fluorescence intensity changes and its standard curve.

[0046] Mix different amounts of organophosphorus pesticide dioxon and 50 μL AChE for 30 min, then add 100 μL 0.1mg / ml ATCh-BNQDs and 50 μL 0.8mM HAuCl 4 solution; the mixed solution was reacted at 37°C for 32 min to measure the fluorescence intensity of the solution, draw the standard curve between the fluorescence intensity and the concentration of dioxon, and calculate the half-inhibitory concentration (IC 50 ),See Figure 5 .

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Abstract

The invention discloses a method for detecting the content of acetylcholinesterase in human serum by using a boron nitride quantum dot-gold nanoparticle nanocomposite. The method comprises the following steps: 1) establishing a standard curve of fluorescence intensities and acetylcholinesterase concentrations: A, preparing iodinated acetylcholine-boron nitride quantum dot solutions; B, respectively adding acetylcholinesterase solutions with the same volumes and different concentrations into the iodinated acetylcholine-boron nitride quantum dot solutions, then adding HAuCl4, and carrying out uniform mixing; and C, after reaction of a series of mixed solutions, respectively determining the fluorescence intensities of the solutions, and drawing a standard curve of the fluorescence intensitiesand the acetylcholinesterase concentrations so as to obtain a linear regression equation; and 2) preparing a mixed solution from a to-be-measured serum sample according to the method prescribed in the sub-step B, determining the fluorescence intensity of the mixed solution, substituting the fluorescence intensity into the linear regression equation to obtain an acetylcholinesterase concentration.The method can be applied to the actual detection of human serum samples, and has the characteristics of simplicity, rapidness and sensitivity.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and nanotechnology, and in particular relates to a method for detecting the content of acetylcholinesterase in human serum by using a boron nitride quantum dot-gold nanoparticle nanocomposite. Background technique [0002] Acetylcholinesterase (AChE) is a very critical enzyme in the process of biological nerve signal transmission. The excitatory effect, thus ensuring the normal transmission of nerve signals in the body. AChE is also involved in the development and maturation of cells, thereby promoting neuronal development and nerve regeneration. Notably, nerve gases, Alzheimer's disease (AD) drugs, and organophosphorus pesticides (OPs) can inhibit the activity of AChE and cause ATCh to accumulate in the synaptic cleft, hindering the transmission of neurotransmitters. Regulating the level of AChE is very important for maintaining normal physiological activities. Therefore, it is of great sig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 郭隆华占园进邱彬林振宇
Owner FUZHOU UNIV
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