A method for detecting the content of acetylcholinesterase in human serum by using boron nitride quantum dot-gold nanoparticle nanocomposite
A technology of acetylcholinesterase and gold nanoparticles, which is applied in the field of analytical chemistry and nanometers, and can solve problems such as time-consuming and low sensitivity
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Embodiment 1
[0031] A method for detecting the content of acetylcholinesterase in human serum by using a boron nitride quantum dot-gold nanoparticle nanocomposite, the specific steps are:
[0032] 1) Synthesis of ATCh-BNQDs
[0033] To 20 mL 0.2mg / mL BNQDs (for characterization see figure 1 ) solution was added 70mg ATCh, mixed and shaken at 37°C for 1h, the mixed solution was passed through a 1000Da dialysis bag to remove unbonded ATCh to obtain ATCh-BNQDs, and whether the product was successfully synthesized was characterized by ultraviolet-visible absorption spectrum, Zeta potential and infrared spectrum ,See figure 2 , and finally quantify the concentration of ATCh-BNQDs by UV-Vis absorption spectroscopy;
[0034] 2) AChE regulates the feasibility of growing AuNPs in situ
[0035] Add 50 μL of different concentrations of AChE to 100 μL of 0.1 mg / ml ATCh-BNQDs solution, followed by 50 μL of 0.8 mM HAuCl 4 Solution, after the mixed solution was reacted at 37°C for 32 minutes, the so...
Embodiment 2
[0045] BNQDs in response to different concentrations of AChE inhibitor dioxon regulated AuNP-induced fluorescence intensity changes and its standard curve.
[0046] Mix different amounts of organophosphorus pesticide dioxon and 50 μL AChE for 30 min, then add 100 μL 0.1mg / ml ATCh-BNQDs and 50 μL 0.8mM HAuCl 4 solution; the mixed solution was reacted at 37°C for 32 min to measure the fluorescence intensity of the solution, draw the standard curve between the fluorescence intensity and the concentration of dioxon, and calculate the half-inhibitory concentration (IC 50 ),See Figure 5 .
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