Plasma nano platform for visual detection and application of plasma nano platform

A plasma and nanotechnology, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of complex operation, time-consuming, expensive instruments, etc., and achieve the effect of ensuring reliability

Active Publication Date: 2018-09-28
SICHUAN UNIV
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Problems solved by technology

However, these methods have disadvantages such as complex operation, time-consuming

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  • Plasma nano platform for visual detection and application of plasma nano platform
  • Plasma nano platform for visual detection and application of plasma nano platform
  • Plasma nano platform for visual detection and application of plasma nano platform

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Embodiment 1

[0074] 1. Construction and detection of plasmonic nano-platforms

[0075] like figure 1 As shown, the plasmonic nano-platform in the present invention consists of three parts: PS nanofiber membrane sensing interface, HIV DNA triggering CHA amplification reaction on the membrane surface and DNAzyme on the membrane surface regulating AuNPs plasma signal. The specific steps are as follows:

[0076] (1) Preparation of PS nanofiber membrane. Using DMF as a solvent, prepare a PS / TBAB / DMF solution with a mass fraction of 10-30%, wherein TBAB is 0.1-0.3% (w / v), and stir at room temperature for 24-26h to obtain an electrospinning solution; The silk solution is spun at a voltage of 15kV, an injection speed of 0.3mL / h, and a receiving distance of 10cm to obtain a fiber film for 1-3 hours; the fiber film is dried in an oven at 60-80°C for 3-6 hours, and then The nanofibrous membrane was cut into a size with a diameter of 5 mm for later use. like figure 2 As shown, the fibrous membran...

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Abstract

The invention provides a plasma nano platform for visual detection and application of the plasma nano platform, and belongs to the field of base mutation detection. The plasma nano platform is prepared from the following components: a hairpin type nucleic acid probe H2 functionalized-nanofiber membrane, a hairpin type nucleic acid probe H1 solution, an MES buffer solution with H2O2 concentration not less than 120 [mu]mol/L, a hemin solution at the concentration between 1 [mu]mol/L and 6 [mu]mol/L, an MES buffer solution containing HAuCl4 at the concentration between 0.2 mmol/L and 0.4 mmol/L,and a glutathione solution at the concentration between 100 [mu]mol/L and 120 [mu]mol/L. The plasma nano platform has the characteristics of simplicity, no marks, supersensitivity and repeated use. The plasma nano platform can detect a targeted DNA at an ultralow concentration in a human serum sample with naked eyes and can increase a concentration distinguishing ratio of a single base to a complete complementary sequence by 0.001 percent, so that the reliability of single base mispairing recognition is guaranteed.

Description

technical field [0001] The invention belongs to the field of base mutation detection, and in particular relates to a plasma nanometer platform for visual detection and an application thereof. Background technique [0002] Single-base mutations in the genome are major contributors to differences in human disease susceptibility and drug response. The concentration of mutated DNA in blood is low (10 -16 ~10 -12 mol / L) and the difference is small compared with the normal expression, it is urgent to develop a highly sensitive single base mutation diagnosis method. [0003] At present, the commonly used single base mutation detection methods mainly include DNA sequencing method, quantitative PCR method, electrochemical method, colorimetric method based on rolling circle amplification (RCA) and so on. However, these methods have disadvantages such as complicated operation, time-consuming and expensive instruments, and are difficult to be widely used in medical diagnosis. The co...

Claims

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Application Information

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IPC IPC(8): C12Q1/6827C12Q1/6834
CPCC12Q1/6827C12Q1/6834C12Q2525/301C12Q2563/131C12Q2563/125C12Q2527/125
Inventor 周翠松尹翠云
Owner SICHUAN UNIV
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