Micro-array protein chip and making method thereof

A protein chip and microarray technology, applied in material testing products, instruments, analytical materials, etc., can solve the problems of high cost, only a few detection units, complicated preparation process, etc., and achieve the effect of no labeling

Inactive Publication Date: 2004-03-17
TSINGHUA UNIV
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Problems solved by technology

[0013] In order to obtain a detectable microarray protein chip with different capacities and overcome the problems of high cost, complicated preparation process and only a few detection units in the existing chip preparation technology, the present invention provides a protein chip capable of high-throughput detection or identification Microarray protein chip of a kind of protein or effector and its interaction and manufacturing method thereof

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  • Micro-array protein chip and making method thereof
  • Micro-array protein chip and making method thereof
  • Micro-array protein chip and making method thereof

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Embodiment Construction

[0031] The microarray protein chip and its manufacturing method according to the present invention will be described in detail below in conjunction with the accompanying drawings.

[0032] Such as figure 2 As shown, the present invention includes an optical glass substrate 5 , a chrome film microarray 6 , a bare gold film microarray 7 , a coupling layer 8 and a receptor 9 fixed together from bottom to top. The optical glass substrate 5 is an optical glass material with a refractive index of 1.5-1.8, its thickness is 0.8-1.2mm, and its size is 0.8×0.8-1.2×1.2mm 2 square piece or Φ0.4-0.6mm disc, smooth on both sides; the chrome film microarray 6 is vapor-deposited or sputtered on one side of the optical glass substrate 5, and the thickness of the chrome film is 2-5nm; the bare gold film The microarray 7 is evaporated or sputtered on the surface of the chromium film microarray 6, the thickness of the bare gold film is 30-50nm, and the number of units of the bare gold film micr...

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Abstract

The present invention provides a micro array protein chip, including optical glass base-plate, chromic film micro array, bare gold film micro array, coupling layer and acceptor, wherein the mercapto end connects with the bare gold film array, the carboxyl end connects with the acceptor. The method for making chips comprises, mask photo-etching of grating, making chromic film or gold film through deposition or sputtering, removing gridding and assembling coupling layer, mounting the acceptor on the coupling layer. The method by the invention can realize the protein chips of various amount units in an effective and low cost way.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a microarray protein chip and a manufacturing method thereof. Background technique [0002] Protein is the smallest active unit that constitutes a living body, that is, the most basic form of life. There are more than 100,000 kinds of proteins in the human body, each of which undertakes different tasks, plays a specific role, and maintains and controls life activities. In different stages of life development, the types and compositions of proteins are different; in different tissues, the proteins expressed by cells vary greatly. A gene is the functional unit of heredity that instructs the synthesis of proteins. Gene mutation, its expression product - protein must be abnormal accordingly, the occurrence and development of any disease will first be expressed at the protein level. Therefore, exploring the laws of life activities at the protein level and studying the mechanism of dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/00G01N33/50G01N33/543G01N33/552G01N33/68
Inventor 余兴龙王鼎新欧阳溅华赵新生
Owner TSINGHUA UNIV
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