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A fully automatic high-throughput optical biosensing device

A biosensing and high-throughput technology, applied in the field of biosensors, can solve problems such as long imaging time, achieve the effects of improving detection efficiency, in-situ real-time detection, and reducing manual operation time and human errors

Active Publication Date: 2017-11-17
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the invention patent (ZL201010128589.5) uses biochips to scan and image in two directions, which takes a long time to image and initially achieves high throughput
So far, oblique incident light reflectance imaging technology has developed rapidly and has been able to achieve high-throughput imaging; however, based on oblique incident light reflectance imaging technology, combined with biochip technology and liquid control technology, fully automatic, label-free, high-throughput Optical biosensing devices for quantitative, real-time measurements remain a challenge

Method used

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  • A fully automatic high-throughput optical biosensing device
  • A fully automatic high-throughput optical biosensing device
  • A fully automatic high-throughput optical biosensing device

Examples

Experimental program
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Embodiment 1

[0078] according to figure 1 As shown, make a fully automatic high-throughput optical biosensing device of the present invention. The monochromatic light generator 1 adopts a helium-neon laser with a wavelength of 632.8nm to output polarized light; the output light of the laser is expanded into collimated light through a beam expander 3, and the beam expansion factor of the beam expander 3 is 3 times; The light passes through the polarizer 5 whose transmission axis is along the p polarization direction; the polarization modulator 6 adopts a photoelastic modulator and is placed along 45°, which can perform high-frequency (50KHz) modulation on the polarization state, and the modulation amplitude is set to 1.8 to obtain Optimum sensitivity; the outgoing light then passes through a phase shifter 7, which uses a zero-order half-wave plate whose fast axis is along the p-polarization direction, and rotating the half-wave plate with the fast axis as the rotation axis can An adjustabl...

Embodiment 2

[0084]Implement according to embodiment 1, the light transmission axis of polarizer 5 and p polarization direction become 45 °; The coefficients are the same; the phase shifter 7 is placed behind the imaging lens 9; the lens 8 uses a cylindrical lens to replace the scanning field mirror and the vibrating mirror, and the focal length of the lens is 5cm; the photodetector 12 uses a diode array containing 152 photodiodes instead of a linear photoelectric Diode; fluid chamber 19, adopting a fluid chamber with multiple parallel small flow chambers instead of a fluid chamber with a single large flow chamber; mechanical translation stage 21, adopting a stepping motor-driven translation stage without an encoder instead of a DC servo motor drive A motorized translation stage with an encoder; sample solution pump 26 and buffer solution pump 24, use a peristaltic pump instead of a syringe pump; biochip 17, use a biochip that prints an oligosaccharide sample for use to detect viruses, bact...

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Abstract

The invention belongs to the technical field of biosensors, in particular to a fully automatic high-throughput optical biosensing device. The device includes: oblique incident light reflection difference imaging unit, sample and liquid processing unit, system control and data acquisition and processing unit. Oblique incident light reflection difference imaging unit includes monochromatic light generator, beam expander, polarizer, polarization modulator, phase shifter, lens, mechanical translation stage, imaging lens, analyzer and photodetector; sample and liquid The processing unit includes a biological chip, a fluid chamber, a buffer solution pump, a sample solution pump, a selection valve and a switching valve; the system control and data acquisition processing unit includes an amplification circuit, an AC weak signal detector and an electronic computer. The device can perform high-throughput, in-situ, real-time and rapid detection of biochips, fully automatic operation, and high detection efficiency. It has a wide range of applications in life science research, high-throughput drug screening, clinical diagnosis, food and environmental safety testing application prospects.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to an optical biosensor device. Background technique [0002] The interaction of biomolecules is the basis of the occurrence of life phenomena. Analyzing the interaction of biomolecules is of great significance for revealing the molecular mechanism of life processes and studying the basic laws of the occurrence and development of life phenomena. In the late 1990s, genomics, proteomics, and metabolomics, which developed rapidly in the late 1990s, studied and explored the phenomenon and essence of life through the overall analysis of genes, proteins, and metabolites. For these complex biological systems, the first major problem to be solved is the high-throughput analysis of biomolecular interactions. Therefore, the development of new methods and devices for high-throughput detection of biomolecular interactions is the key to major progress and breakthroughs in the fie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00
Inventor 费义艳朱湘东吉姆·兰德里
Owner FUDAN UNIV
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