Hybridization system for real-time monitoring parsing process of micro array chip

Abstract

The invention discloses a hybridization system for real-time monitoring parsing process of a micro array chip, which comprises a hybridization cavity body, a temperature control device, a peripheral flow path and a real-time imaging module, wherein the hybridization cavity body is composed of the micro array chip, a cavity body cofferdam and a cover plate, and a fluid inlet and a fluid outlet are formed on the cover plate; the temperature control device is connected with the hybridization cavity body; the peripheral flow path is communicated with the hybridization cavity body by the fluid inlet and the fluid outlet; and the real-time imaging module comprises a light source and a photodetection device, and the light of the light source irradiates the surface of the micro array chip, reflected by the surface of the micro array chip and then projected to the photodetection device. The micro array chip hybridization system can realize dynamic hybridization, cleaning, drying and other flows of the chip; and the real-time imaging module can realize real-time monitoring on the magnetic bead intensity of the processing flows of the chip. According to the signal change condition, the chip processing condition is optimized; and the hybridization system can also be used for studying the dynamic problems of solid-liquid phase hybridization.

Description

technical field [0001] The invention relates to a hybridization system, in particular to a hybridization system for real-time monitoring of the microarray chip analysis process. Background technique [0002] Microarray chips are formed by immobilizing a large number of probe molecules with known sequences on the support in an orderly and high-density manner by means of micro-processing technology. The probe molecules can be DNA, RNA, protein, sugar, etc. By detecting whether the probe hybridizes with the target substance in the sample, gene expression characteristics, genetic defects, protein distribution, reaction characteristics, etc. can be analyzed. [0003] The analysis method based on microarray chips mainly includes steps such as sample pretreatment, chip preparation, chip hybridization, chip cleaning and drying, and chip detection. The current chip analysis platform includes two types: one type of platform uses large-scale equipment to complete processing steps such...

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Problems solved by technology

Since the signal changes on the chip surface cannot be monitored in real

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