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A quality evaluation, honeysuckle technology, applied in the field of medical testing, can solve the problem of single evaluation standard components, etc., to achieve the effect of improving quality control level, high detection sensitivity, and improving reliability and effectiveness
Active Publication Date: 2016-12-21
SHANDONG ANALYSIS & TEST CENT
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Problems solved by technology
At present, there are existing research reports on the quality evaluation of honeysuckle, and most of them are carried out with reference to the Pharmacopoeia, and the evaluation standard components are relatively single.
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Embodiment 1
[0031] A method for evaluating the quality of honeysuckle, comprising measuring chlorogenic acid, luteolin and six iridoid glycoside components in honeysuckle, wherein the six iridoid glycoside components are loganic acid, morroniside , loganin, dangyaoside, broken-oxygenated loganin, and bistroside dimethyl acetal; the determination methods of chlorogenic acid and luteolin were carried out in accordance with the 2015 edition of "Chinese Pharmacopoeia". The instrument materials and method steps for detecting the six iridoid glycosides are listed in detail as follows.
[0032] 1 Instruments and materials
[0033] Agilent 1260 high performance liquid chromatograph, Agilent Company of the United States; KQ2400KDE high-power numerically controlled ultrasonic instrument, Kunshan Ultrasonic Instrument Co., Ltd.; R201 rotary evaporator, Shanghai Shensheng Technology Co., Ltd.; BSA1245-CW precision balance, Germany Sartorius Company; Milli-Q (18.2MΩ) ultrapure water treatment system,...
Embodiment 2
[0050] As described in Example 1, another honeysuckle sample 2 was selected, and the experimental results are shown in Table 2.
[0051] The determination result of iridoid glycosides in the sample of table 2
[0052]
[0053] According to the method described in the 2015 "Chinese Pharmacopoeia", the chlorogenic acid and luteolin in the honeysuckle No. 2 sample were determined: the content of chlorogenic acid was 31.5 mg / g, and the content of sweet clover licorice was 0.815 mg / g.
Embodiment 3
[0055] As described in Example 1, another honeysuckle sample 3 was selected, and the experimental results are shown in Table 3.
[0056] The determination result of iridoid glycosides in the sample of table 3
[0057]
[0058] According to the method described in the 2015 "Chinese Pharmacopoeia", the chlorogenic acid and luteolin in the honeysuckle No. 2 sample were determined: the content of chlorogenic acid was 28.6 mg / g, and the content of sweet clover licorice was 0.735 mg / g.
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Abstract
The invention discloses a honeysuckle quality evaluation method. According to the honeysuckle quality evaluation method, chlorogenic acid, galuteolin and six iridoid glycoside ingredients in honeysuckle are determined, wherein the six iridoid glycoside ingredients are loganic acid, morroniside, loganin, chiratin, secoxyloganin and secologanin dimethyl acetal. The honeysuckle quality evaluation method disclosed by the invention has the advantages that the iridoid glycoside ingredients in the honeysuckle are included in a honeysuckle quality evaluation system for the first time, six representative iridoid glycoside ingredients in the honeysuckle are selected and determined at the same time for guaranteeing reliability and effectiveness of the quality evaluation method, and a dispersive solid phase extraction-high performance liquid chromatography (DSPE-HPLC) is established for the first time and used for detecting the six iridoid glycoside ingredients of different structures, so that reliability and effectiveness of honeysuckle quality evaluation are obviously improved, quality control level of traditional Chinese medicine honeysuckle and related patent medicines is further improved, and the honeysuckle quality evaluation method disclosed by the invention is significant on guidance of clinical application.
Description
technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a method for evaluating the quality of honeysuckle. Background technique [0002] Honeysuckle, also known as double flower, two flower, soil silver flower, honeysuckle flower, etc., is the dry flower bud or the first flower of Lonicera japonica Thunb., which is widely distributed in various provinces of my country. It has a long history of application and is commonly used in my country. One of the bulk of traditional Chinese medicinal materials, first recorded in Liang Dynasty Tao Hongjing's "Famous Doctors", sweet in taste and cold in nature, it belongs to the lung, heart and stomach meridian, has the functions of clearing away heat and detoxification, and dispersing wind-heat, mainly used for treating carbuncles and furuncles , sore throat, erysipelas, heat-toxin blood dysentery, wind-heat cold, febrile disease fever embolism. [0003] There are many factor...
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