Method for preparing microarray chip between hydrophilic phase and hydrophobic phase and carrying out mass spectrum imaging quantitative analysis by using microarray chip

Abstract

The invention belongs to the technical field of chemical analysis and discloses a method for preparing a microarray chip between a hydrophilic phase and a hydrophobic phase and carrying out mass spectrum imaging quantitative analysis by using the microarray chip. The method comprises the following steps: selecting a hydrophobic polymer; printing the hydrophobic polymer on conductive glass by adopting a screen printing technique according to a designed template to form a hydrophilic region and a hydrophobic region which are clear and uniform; uniformly paving a water solution of a mixture system to be detected in the hydrophilic region to form a chip; drying and curing the chip; uniformly paving a base material solution for ionizing the water solution of the mixture system to be detected on the surface of the chip; after the chip is dried, forming a sample; measuring a mass spectrum image of the sample by using a mass spectrum imaging method; processing the image obtained by mass spectrum imaging of the sample in the same area through using a mathematical method, and carrying out the quantitative analysis. The method is high in automation degree, simple in operation and high in sensitivity; the detection sensitivity of a material to be detected reaches 10-15mol / L; the use amount of the sample is less and is as low as a microliter-grade.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, in particular to a method for preparing a micro-array chip with alternate hydrophilic and hydrophobic phases and its application in mass spectrometry imaging quantitative analysis. Background technique [0002] Microarray chips (Microarray) are characterized by high-density arrays. Its basic research began in the late 1980s, which is essentially a kind of biotechnology, mainly developed in the field of biogenetics. Microarray technology is to use the principle of molecular hybridization to hybridize the samples being compared with the microarray at the same time, and convert them into the abundance of specific genes in different samples by detecting the hybridization signal intensity and data processing, so as to comprehensively compare the gene expression of different samples level of difference. Microarray chips have become the fastest-growing part of the chip field because of their...

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Problems solved by technology

Spin coating technology is not easy to obtain a microporous structure with hydrophilic and hydrophobic phases due to its realization principle, and is most suitable for making a uniform planar two-dimensional structure; electrospinning technology is simple, but there are gaps between the prepared nanofibers, and it is impossible to obtain complete affinity and hydrophobicity. The structure between the water and the phase is easy to cause the leakage of the hydrophilic substance to be tested

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