36results about How to "Enhanced SERS signal" patented technology

The invention relates to a gold nanoparticle-molybdenum disulfide-gold ultra-sensitive SERS substrate material and a preparation method thereof. The structure is a gold nanoparticle / molybdenum disulfide / gold sandwich structure with the gold nanoparticle as a core, the molybdenum disulfide as a shell, and a layer of gold particles with a certain "hot sopt" effects evaporated on the surface of the core-shell structure in a vacuum mode. The molar ratio of the nano-material element is molybdenum: sulfur: gold being 1.0:1.9: 1.0. The chemical formula of molybdenum disulfide is MoS2. Compared with the prior art, the preparation method of the gold nanoparticle-molybdenum disulfide-gold ultra-sensitive SERS substrate material is convenient and fast. According to the gold nanoparticle-molybdenum disulfide-gold SERS substrate material, MoS2 serves as a supporting body to adsorb target molecules, a double-plasma coupling effect exists between the Au nano particles, the "hot spot" effect enhancedan SERS signal, the MoS2 is in bridge connection with the Au so as to realize the ultra-sensitive detection of rhodamine B molecules, the detection limit can reach 10-10 mol / L, and the repeatability and the stability of the rhodamine B is detected to be good.

The invention discloses a composite nanomaterial, a preparation method and application thereof, a surface enhanced Raman scattering (SERS) substrate, a detection device and a detection method. Throughthe composite nanomaterial, the SERS substrate can have better sensitivity. The composite nanomaterial comprises a two-dimensional MXene nanosheet and a one-dimensional metal nanomaterial adsorbed onthe two-dimensional MXene nanosheet. According to the nanomaterial, through an adsorption function, the one-dimensional nano-metallic material is uniformly loaded on the two-dimensional nanosheet, many hotspots can be generated after loading due to an extremely high specific surface area and physico-chemical properties of the nanomaterial, and the purpose of enhancing an SERS signal is achieved;and the nanomaterial has sensitive Raman detection performance, the content of pesticide residues can be quickly detected, and the pesticide detection limit can reach 10<-10>mol / L. The prepared composite nanomaterial has good detection reproducibility when used for detecting the Raman substrate and can be widely applied to detection of other organic pollutants, for example, food additives.

The invention discloses a phenol recognition SERS probe, a preparation and an application thereof, and a universal ultra-sensitive immunoassay method based on SERS. Firstly, a phenol responsive SERS probe is prepared by reducing chloroauric acid with DTDBA, ahen, ELISA is combined with SERS, biomolecules are labeled with ALP, a substrate PPNA is hydrolyzed by ALP to generate phenol. An SERS probesignal caused by the phenol is used for realizing a technology for sensitive detection of the biomolecules. The technology can not only overcome the disadvantages of low sensitivity of the conventional enzyme linked immunosorbent assay, but also can solve the problems of enhancement and poor reproducibility of SERS detection signals. The phenol recognition SERS probe disclosed by the invention hasremarkable universality, can be widely used for immunoassay using the ALP as an enzyme label to measure a variety of biomolecules, lays a solid foundation for the development of immune technology based on SERS detection, and has a very large development space and a broad application prospect in the fields of biological and chemical detection, medical diagnosis, and the like.

The invention provides a preparation method of an Ag-SiO2-Ag nanosphere array. The method comprises the following steps: forming a layer of Ag thin film on the surface of a monocrystalline silicon sample wafer by virtue of thermal vacuum evaporation; putting the monocrystalline silicon sample into a chemical vapor deposition reaction chamber for heating to a preset temperature, so that the Ag thin film is dehumidified in a solid state and formed into Ag nanoparticles; introducing a reactant gas silane at the preset temperature so that a layer of extremely thin silicon nano-film can be evenly deposited on the surface of the Ag nanoparticles, thereby obtaining an Ag-Si core-shell nanosphere array structure; next, taking out the monocrystalline silicon sample and putting into an oxygen atmosphere for heating so that the Si nano-shell can be oxidized into SiO2; further performing thermal vacuum evaporation to form an Ag thin film with a preset thickness on the surface of the sample, thereby forming the Ag-SiO2-Ag core-shell nanosphere array structure which can be taken as a surface-enhanced Raman scattering (SERS) substrate. The invention aims at providing a technical scheme of the preparation method of the Ag-SiO2-Ag nanosphere array having the SERS effect.

The present disclosure provides a bimetallic nanostructure with stimuli-responsiveness, including: a metal seed; a nanogap including a dopamine-modified stimuli-responsive copolymer attached to only a portion of the metal seed; and a metal shell surrounding the nanogap. The bimetallic nanostructure has a controllable interior nanogap, and may be used as a surface-enhanced Raman scattering (SERS) nanoprobe with improved SERS signals by virtue of the presence of the interior nanogap.

The invention discloses a lateral flow immune test strip based on an ordered micro-nano structure. The lateral flow immune test strip is used for detecting a target in an analyte. According to the test strip, a base is used as a substrate, a sample pad, a conjugate pad, a chromatography pad, an ordered micro-nano structure detection pad and an absorption pad are sequentially distributed on the substrate from left to right, the sample pad is a target loading area, nanoparticles capable of being coupled with a target are combined on the conjugate pad, the ordered micro-nano structure detection pad is arranged on the chromatography pad, and a detection area and a quality control area are distributed on the ordered micro-nano structure detection pad; the sample pad and the conjugate pad are overlapped together, the conjugate pad and the chromatography pad are overlapped together, the ordered micro-nano structure detection pad is fixed on the chromatography pad, and the chromatography pad and the absorption pad are overlapped together; in practical application, when a sample reaches a detection area, multi-target detection can be realized by collecting signals; the test strip has the advantages of short detection time, simplicity and convenience in operation, low cost, simultaneous detection of multiple targets and the like, and is expected to be widely applied to the field of rapiddetection.

The invention belongs to the field of surface-enhanced Raman scattering effects of silicon wafers, and discloses a preparation method of a salt bridge-assisted primary battery growth-induced gold nanoparticle surface-enhanced Raman scattering substrate. The preparation method comprises the following steps: 1, preparing a gold seed crystal colloidal solution from chloroauric acid, sodium citrate and sodium borohydride; 2, taking agar and potassium chloride, adding distilled water, dissolving, and then pouring into a U-shaped tube to obtain an agar-potassium chloride salt bridge; 3, dropwise adding the gold seed crystal colloidal solution onto the surface of ITO conductive glass to obtain an ITO conductive glass substrate spin-coated with gold seed crystals; 4, connecting graphite with the ITO conductive glass substrate by using a conductive wire, placing the graphite in a ferrous chloride solution, placing the ITO conductive glass substrate in a depositing solution, inserting the agar-potassium chloride salt bridge into the two solutions, and standing for 3 hours; 5, sputtering silver onto the ITO conductive glass substrate to obtain the gold nanoparticle surface-enhanced Raman scattering substrate. The preparation method is simple, the preparation time is short, and the prepared gold nanoparticle substrate has very good SERS activity and uniformity and high detection sensitivity.

Provided herein are substrates useful for surface-enhanced Raman spectroscopy (SERS), as well as methods of making substrates. The substrates comprise a support element; a nanoparticulate layer; a SERS-active layer in contact with said nanoparticulate layer; and optionally, an immobilizing layer disposed between said nanoparticulate layer and said support element; wherein if the optional immobilizing layer is not present, the nanoparticulate layer is thermally bonded to the support element; and if said optional immobilizing layer is present, said nanoparticulate layer thermally bonded to said immobilizing layer, and optionally, further thermally bonded to said support element. In addition, methods of making the substrates, along with methods of detecting and increasing a Raman signal using the substrates, are described herein.

The invention discloses preparation of an SERS (Surface Enhanced Raman Scattering) substrate and application of the SERS substrate to cancer detection and belongs to the field of nanomaterials. The SERS substrate is TiO2 inverse opal plated with 80nm Au, and the bore diameter of the TiO2 inverse opal is 290nm. In the SERS substrate, the bore diameter of the TiO2 inverse opal is matched with the diameter (about 90nm) of an exosome of a to-be-detected object, and the exosome can make full contact with the SERS substrate; and on the other hand, the TiO2 inverse opal with the bore diameter being 290nm can generate a slow light effect during analyte detection under the excitation of a 633nm laser, and then an SERS signal of the exosome itself can be better obtained. By use of the SERS substrate, the phosphorylation degree of exosome protein extracted from plasma can be quickly analyzed, and then a cancer is diagnosed; consumed time is short, and sensitivity is high; and a new thought is provided for quick diagnosis of a non-invasive in-vitro cancer.

The invention discloses a method for synthesizing a silver substrate and application of the method, and relates to the technical field of research of surface phenomena related to adsorption molecules, particularly a synthesizing technology for a metallic nano material substrate used as a probe molecule in SERS detection. The method comprises the following steps: adopting a hydro-thermal synthesis method, using silver nitrate as a silver source, using urea as an ammonia source, using formaldehyde as a reducer, changing the pH value of a solution through the urea, under the hydro-thermal condition, gradually reducing silver, and then preparing the silver substrate, wherein the silver substrate can be used as the substrate for researching surface enhanced raman scattering (SERS). The method is simple, convenient and fast, the used raw materials are relatively cheap, the synthesized product is stable in surface and convenient to store, and the method can be directly used for product representation and SERS detection.

The invention relates to a method for detecting the content of dioxane in children cosmetics. The method comprises the following steps: (1) pretreating a sample to be detected so as to form a sample solution; (2) uniformly mixing the sample solution with prepared nanometer silver colloid so as to form a sample mixed solution; (3) dropping the sample mixed solution on a substrate, and naturally airing, thus obtaining a detection sample; (4) detecting the detection sample by adopting surface-enhanced Raman scattering, thus obtaining an SERS (surface enhanced Raman spectrum) spectrogram of the detection sample; and (5) comparing the obtained SERS spectrogram of the detection sample with an SERS spectrogram of a dioxane standard solution, and calculating so as to obtain the content of the dioxane in the detection sample. The method is simple, convenient and fast to operate and can be used for rapidly carrying out qualitative and quantitative analysis on the content of the dioxane in children cosmetics.

The invention discloses gold nanoparticles as well as a preparation method and application thereof. The gold nanoparticles are used for enhancing the SERS effect and are spinous gold nanoparticles or triangular gold nanoparticles, spinous structures are arranged on the surfaces of the spinous gold nanoparticles, and the particle size of the particles ranges from 60 nm to 165 nm; the triangular gold nanoparticles are in the shape of a regular triangle, and the side length of the triangular gold nanoparticles ranges from 60 nm to 70 nm. The invention also provides a preparation method and application of the particle, AuNPs with special morphology is used as an SERS substrate, based on the special size morphology and surface structure of the AuNPs, the SERS signal of a detection sample can be significantly enhanced, and the particle has excellent sensitivity, detection limit and repeatability stability for detection of parathion-methyl.

The invention discloses preparation and application of a Fe3O4@GO@Ag and gold nanostar SERS substrate. The preparation comprises the steps of synthesizing magnetic ferroferric oxide nanoparticles by an improved solvothermal method, carrying out amination modification on the magnetic ferroferric oxide nanoparticles, and coating a graphene oxide shell on the outer layer by electrostatic adsorption,wherein silver nanoparticles can be nucleated and grown in situ by a large specific surface area and a large number of oxygen-containing functional groups on the surface, so that a core-shell satellite structure is realized; the graphene oxide has obvious Raman peaks (G peak, D peak and 2D peak) and can be used as a stable internal standard to reduce experimental errors; the gold nanostars are synthesized through simple chemical reduction and are connected to Raman beacon molecules with Raman signals in a biological silence area through gold-sulfur bonds; and after the two substrates are modified with a primary antibody and a secondary antibody respectively, rapid and sensitive detection of the antigen can be realized. The method is simple in synthesis, easy to separate and high in sensitivity.

The invention discloses a design method of an SERS substrate based on a surface plasmon polariton effect, which comprises the following steps of: setting a structure of an SERS substrate, including aSiO2 substrate layer, an Au metal film layer and a SiO2 dielectric layer, by adopting a time domain finite difference algorithm; determining initial conditions of simulation; sequentially changing thethickness of the Au metal film layer, the thickness of the SiO2 dielectric layer, the thickness of the Au top metal layer and the gap size of the double-circular-hole structure, calculating the electric field intensity distribution of the SERS substrate by utilizing FDTD solution software, calculating an enhancement factor of the SERS substrate according to the electric field intensity distribution, and screening out a structure with a relatively large enhancement factor as the optimized SERS substrate. According to the structure designed by the invention, incident light energy can be effectively converted into surface plasma wave energy, and effective coupling of LSPR and PSPR is generated by etching the composite film SERS substrate of the nano double-hole ordered array, so that an SERSsignal is enhanced to the maximum extent.

The invention relates to a preparation method for a nano gold-silver composite material substrate of a core-shell structure, and belongs to the technical field of surface-enhanced Raman scattering (SERS). The preparation method comprises the following steps that HAuCl4 is reduced into elemental gold by using Na3C6H5O72H2O by means of a hydrothermal reduction method, and cleaning and dispersing are conducted to obtain Au seed solutions; the Au seed solutions, Na3C6H5O72H2O solutions and ascorbic acid solutions are added into deionized water, uniformly stirred, and subjected to a water bath at 40 DEG C for 15 minutes; silver nitrate solutions are added dropwise at a constant speed, continuously stirred, and subjected to a water bath at 40 DEG C for 30-40 minutes; nano gold-silver composite material sol of a core-shell structure is obtained after cleaning; and the sol is dripped on a clean silicon wafer and covers the surface of the silicon wafer, vacuum drying is performed, and the substrate is prepared. According to the preparation method, the problem of remarkable anisotropism of single noble metal nanoparticles is greatly solved; and the SERS signal on the substrate is greatly enhanced, and the lowest detection limit of the substrate is 109M.

The invention relates to a nucleic acid structure detection method based on surface enhanced Raman spectroscopy. The nucleic acid structure detection method comprises: (1) preparing a silver nanoparticle sol modified and cleaned with iodine ions; and (2) adding a to-be-detected DNA sample to a centrifuge tube filled with the iodine ion modified silver nanoparticle, adding a buffer solution with the PH value of 3.0-5.0, adding an aluminum ion or titanium ion aggregating agent, and carrying out surface enhanced Raman spectroscopy detection. According to the present invention, the method effectively detect the secondary structure of nucleic acid, has the advantages of simpleness, rapidness and the like of SERS, and further has extremely high reproducibility and extremely high reliability; themethod overcomes the difficulty in the detection of the secondary structure of nucleic acid in the traditional SERS detection, has advantages of simple operation steps, rapidness, high sensitivity and good reproducibility, can obtain the signal with the extremely high signal-to-noise ratio, and achieves the high sensitivity detection on various nucleic acid secondary structures.

Object: To provide a biochip for use in exhaustive analysis of a particular protein including DNA (deoxyribose nucleic acid) in a body fluid through Raman quantitative analysis.Resolving Means: Aqueous solution of metal complexes including plasmon metal selected from the group consisting of Au, Ag, Pt and Pd is supplied dropwise onto a carrier metal having an electrode potential of metal less noble than complex metal, followed by precipitation of nanometric quantum crystals from the metal complex on the carrier metal, the metal complex being so selected as to have a complex stability constant (log β) that is expressed by the following equation (I) correlating with the electrode potential E of the carrier metal:E°=(RT / |Z|·F)In(βi)  (I)(wherein E° represents the standard electrode potential, R represents a gas constant, T represents the absolute temperature, Z represents the ion valency, and F represents the Faraday constant), the surface property of the metal complex quantum crystals on the carrier metal being subsequently adjusted in dependence on an object to be detected in the aqueous solution prior to the precipitation or after the precipitation.

The invention discloses a phenol recognition SERS probe, its preparation and application, and a SERS-based general ultrasensitive immune analysis method. First, DTDBA was used to reduce chloroauric acid to prepare a phenol-responsive SERS probe, then ELISA was combined with SERS, ALP was used to label biomolecules, and ALP was used to hydrolyze its substrate PPNa to produce phenol, and the SERS probe signal caused by phenol was thus Technologies to achieve sensitive detection of biomolecules. This technology can not only overcome the shortcomings of low sensitivity of conventional enzyme-linked immunoassays, but also solve the problems of SERS detection signal enhancement and poor reproducibility. The invention has remarkable universality, can be widely used in immunoassays using ALP as an enzyme marker to measure various biomolecules, and lays a solid foundation for the development of immune technology based on SERS detection, and can be used in the fields of biology, chemical detection, medical diagnosis, etc. All have great development space and broad application prospects.

The invention provides a preparation method of an Ag-SiO2-Ag nanosphere array. The method comprises the following steps: forming a layer of Ag thin film on the surface of a monocrystalline silicon sample wafer by virtue of thermal vacuum evaporation; putting the monocrystalline silicon sample into a chemical vapor deposition reaction chamber for heating to a preset temperature, so that the Ag thin film is dehumidified in a solid state and formed into Ag nanoparticles; introducing a reactant gas silane at the preset temperature so that a layer of extremely thin silicon nano-film can be evenly deposited on the surface of the Ag nanoparticles, thereby obtaining an Ag-Si core-shell nanosphere array structure; next, taking out the monocrystalline silicon sample and putting into an oxygen atmosphere for heating so that the Si nano-shell can be oxidized into SiO2; further performing thermal vacuum evaporation to form an Ag thin film with a preset thickness on the surface of the sample, thereby forming the Ag-SiO2-Ag core-shell nanosphere array structure which can be taken as a surface-enhanced Raman scattering (SERS) substrate. The invention aims at providing a technical scheme of the preparation method of the Ag-SiO2-Ag nanosphere array having the SERS effect.

The invention relates to a method for detecting the content of dioxane in children cosmetics. The method comprises the following steps: (1) pretreating a sample to be detected so as to form a sample solution; (2) uniformly mixing the sample solution with prepared nanometer silver colloid so as to form a sample mixed solution; (3) dropping the sample mixed solution on a substrate, and naturally airing, thus obtaining a detection sample; (4) detecting the detection sample by adopting surface-enhanced Raman scattering, thus obtaining an SERS (surface enhanced Raman spectrum) spectrogram of the detection sample; and (5) comparing the obtained SERS spectrogram of the detection sample with an SERS spectrogram of a dioxane standard solution, and calculating so as to obtain the content of the dioxane in the detection sample. The method is simple, convenient and fast to operate and can be used for rapidly carrying out qualitative and quantitative analysis on the content of the dioxane in children cosmetics.

The invention discloses a surface enhanced Raman scattering probe based on a carbon nanotube and a preparation method thereof. By adopting the structural characteristics of the carbon nanotube, the surface enhanced Raman scattering signal intensity of the probe is raised. The probe comprises a core layer, a coating layer adsorbing around the core layer and a surface enhanced Raman scattering marker adsorbing around the coating layer, wherein the core layer comprises a carbon nanotube coated with a layer of positive polypropylene amine hydrochloride, and the coating layer is a metal nanoparticle layer composing of metal nanoparticles. According to the invention, the surface enhanced Raman scattering probe with a specific structure is prepared by using the metal nanoparticles as a surface enhanced Raman scattering substrate and using the carbon nanotube as a loading template; under the irradiation of excitation lights, the probe has surface enhanced Raman scattering signals with high signal to noise ratio.

The invention discloses a preparation method and an application of a SERS biosensor for detecting a tumor marker miRNA 141. The method comprises the following steps: (1) combining a Fe3O4@Au magneticnanoparticle with a capture probe to obtain a capture unit solution; (2) combining a hollow gold nanosphere with an initiation probe to prepare a signal unit solution; and (3) reacting and combining acapture unit, miRNA 141 and a signal unit, and performing magnetic separation to obtain the SERS biosensor. The application is characterized in that an SERS signal is detected by a Raman spectrometer, SERS signal intensities corresponding to a series of miRNA-141 with different concentrations are detected, and a quantitative relationship between the concentration of the miRNA-141 and the SERS signal intensity is established; and the concentration of miRNA-141 in an unknown sample is detected according to the quantitative relationship. The SERS biosensor has the advantages of high sensitivity,high specificity, simple assembling process and fast detection speed.

The invention relates to a spectral method capable of exciting surface-enhanced Raman spectroscopy (SERS) in a long range surface plasmon mode. The method comprises the following steps of: constructing a buffer layer, a metal layer and a protective layer on the bottom surface of a prism to form a long range surface plasmon resonance (LRSPR) device; placing the LRSPR device with multilayer structure under the irradiation of a laser source, and adjusting the incident angle of the laser source to an LRSPR angle; and in a specific incident direction, generating the LRSPR, so that the electric magnetic field on the surface of a metal is enhanced, and the excitation process of the surface-enhanced Raman spectroscopy of a detected object of a deeper area in a sample layer is completed. Because the long range effect has deeper penetrating effect, the construction of the protective layer on the surface of the metal layer becomes possible. The transduction membrane made of chemically inert goldor platinum is changed into a silver membrane with lower cost, oxidation resistance and better enhancing effect. The LRSPR-mechanism-based SERS detection method has great significance.

The invention discloses an integrated nano-reagent for cancer diagnosis and treatment, including a miRNA identification probe prepared by modifying a Y-shaped DNA structure on the surface of AuNP, and a miRNA identification probe prepared by modifying a double-stranded DNA linker and a Raman molecule on AuNP For the obtained SERS probe, the invention also discloses the preparation method and application of the above-mentioned integrated reagent for cancer diagnosis and treatment. The integrated nano-reagent for cancer diagnosis and treatment of the present invention, driven by ATP in cancer cells, the specially designed Y-shaped DNA structure and double-stranded DNA linker on AuNP will undergo conformational transformation triggered by miRNA-106a, releasing miRNA- 106a and cyclic amplification, triggering the SERS signal to significantly enhance the recognition of cancer cells; at the same time, the DNAzyme generated and amplified by the conformational switch can catalyze the cleavage of Survivin mRNA and c-Jun mRNA, realizing effective dual gene silencing therapy.

The invention relates to a gold nanoparticle-molybdenum disulfide-gold ultra-sensitive SERS substrate material and a preparation method thereof. The structure is a gold nanoparticle / molybdenum disulfide / gold sandwich structure with the gold nanoparticle as a core, the molybdenum disulfide as a shell, and a layer of gold particles with a certain "hot sopt" effects evaporated on the surface of the core-shell structure in a vacuum mode. The molar ratio of the nano-material element is molybdenum: sulfur: gold being 1.0:1.9: 1.0. The chemical formula of molybdenum disulfide is MoS2. Compared with the prior art, the preparation method of the gold nanoparticle-molybdenum disulfide-gold ultra-sensitive SERS substrate material is convenient and fast. According to the gold nanoparticle-molybdenum disulfide-gold SERS substrate material, MoS2 serves as a supporting body to adsorb target molecules, a double-plasma coupling effect exists between the Au nano particles, the "hot spot" effect enhancedan SERS signal, the MoS2 is in bridge connection with the Au so as to realize the ultra-sensitive detection of rhodamine B molecules, the detection limit can reach 10-10 mol / L, and the repeatability and the stability of the rhodamine B is detected to be good.

The invention discloses a cancer diagnosis and treatment integrated nano reagent which comprises a miRNA recognition probe prepared by modifying the surface of AuNP with a Y-type DNA structure and an SERS probe prepared by modifying AuNP with a double-stranded DNA connector and Raman molecules, and the invention further discloses a preparation method and application of the cancer diagnosis and treatment integrated reagent. According to the cancer diagnosis and treatment integrated nano reagent disclosed by the invention, under the driving of ATP (Adenosine Triphosphate) in cancer cells, a Y-type DNA structure specially designed on AuNP and a double-stranded DNA connector are subjected to conformational transformation triggered by miRNA-106a, miRNA-106a is released and circularly amplified, and SERS (Surface Enhanced Raman Scattering) signals are initiated to remarkably enhance the recognition of the cancer cells; meanwhile, DNAzyme generated and amplified through conformation conversion can catalytically crack Survivin mRNA and c-Jun mRNA, and effective double gene silencing treatment is achieved.

The invention provides a Raman sensing analysis method for detecting enterotoxin, which belongs to the technical field of optical analysis. Comprise the following steps: cesium bromide (CsBr) and lead bromide (PbBr 2 ) powder doped in mesoporous silica (MSNs) to prepare sterically hindered CsPbBr via high-temperature desolvation 3 @MSNs. Ag islands were grown on the Au core via the ligand 2‑mercaptobenzimidazole‑5‑carboxylic acid (MBIA) to synthesize Au‑Ag Janus NPs. Furthermore, the ligand MBIA acts as a Raman beacon molecule, exhibiting a very unique and stable Raman signal. Au‑Ag Janus NPs and CsPbBr 3 @MSNs can greatly amplify the SERS signal through the composite material formed by antigen-antibody. The Raman signal of MBIA is used as the detection signal to avoid additional modification of the Raman beacon. The detection method of the invention has high sensitivity and strong specificity.