Surface enhanced Raman scattering probe based on carbon nanotube and preparation method thereof

A technology of surface-enhanced Raman and carbon nanotubes, applied in the fields of nanomaterials and bioanalytical chemistry, can solve the problem of further improvement in sensitivity, stability and biocompatibility, low signal intensity of surface-enhanced Raman scattering, and biocompatibility Poor performance and other problems, to achieve the effect of low price, environmental friendliness and low cost

Inactive Publication Date: 2014-08-06
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Although there have been a lot of reports on the structure and preparation methods of SERS probes, there are not many probes suitable for living organisms, and the preparation methods are relatively cumbersome, and the sensitivity, stability and biocompatibility need to be further improved.
At present, most studies based on carbon nanotube / metal nanoparticle composite structure are applied in the field of electrochemical detection, and there are few studies as SERS probes. They mainly use long-chain single-walled or multi-walled carbon nanotubes as templates, through covalent Bonded metal nanoparticles, the preparation method is cumbersome, the stability is poor, the signal intensity of surface-enhanced Raman scattering is low, and because of its large size, the biocompatibility is poor

Method used

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  • Surface enhanced Raman scattering probe based on carbon nanotube and preparation method thereof
  • Surface enhanced Raman scattering probe based on carbon nanotube and preparation method thereof
  • Surface enhanced Raman scattering probe based on carbon nanotube and preparation method thereof

Examples

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Embodiment 1

[0044] Embodiment 1: Prepare the optical probe with the carbon nanotubes modified gold-silver composite nanoparticles as the SERS substrate, and the 5,5-dithiobis-2-nitrobenzoic acid molecule as the SERS marker. The method includes the following steps :

[0045] 1) Preparation of water-soluble carboxyl-functionalized carbon nanotubes.

[0046] Add 50 mg of purchased original multi-walled carbon nanotubes (diameter 40-60 nm, length 1-2 μm) to 100 mL of sulfuric acid with a concentration of 96% to 98% and nitric acid with a concentration of 65% to 68% (v / v=3: 1, 2:1 or 3:1) in the mixed solution, and then after ultrasonic cleaning for 8 hours, use a large amount of deionized water to filter and wash in the suction filter bottle until it is close to neutral, and the obtained carboxylated carbon nanotubes The length is about 500nm or so.

[0047] 2) Preparation of gold nanoparticles.

[0048] Get 1mL of 1% chloroauric acid solution and add it to 100mL of deionized water, heat t...

Embodiment 2

[0057] Example 2: The preparation method and steps are the same as those in Example 1, except that 5,5-dithiobis-2-nitrobenzoic acid molecules are replaced by 4-mercaptobenzoic acid molecules.

Embodiment 3

[0058] Example 3: Preparation of SERS optical probes with carbon nanotubes modified with silver nanoparticles as SERS substrates and 5,5-dithiobis-2-nitrobenzoic acid molecules as surface-enhanced Raman scattering markers, including Follow the steps below:

[0059] 1) Preparation of water-soluble carboxyl-functionalized carbon nanotubes. See example 1 step 1)

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Abstract

The invention discloses a surface enhanced Raman scattering probe based on a carbon nanotube and a preparation method thereof. By adopting the structural characteristics of the carbon nanotube, the surface enhanced Raman scattering signal intensity of the probe is raised. The probe comprises a core layer, a coating layer adsorbing around the core layer and a surface enhanced Raman scattering marker adsorbing around the coating layer, wherein the core layer comprises a carbon nanotube coated with a layer of positive polypropylene amine hydrochloride, and the coating layer is a metal nanoparticle layer composing of metal nanoparticles. According to the invention, the surface enhanced Raman scattering probe with a specific structure is prepared by using the metal nanoparticles as a surface enhanced Raman scattering substrate and using the carbon nanotube as a loading template; under the irradiation of excitation lights, the probe has surface enhanced Raman scattering signals with high signal to noise ratio.

Description

technical field [0001] The invention belongs to the fields of nanomaterials and bioanalytical chemistry, and in particular relates to a preparation method of a surface-enhanced Raman scattering probe. Background technique [0002] In recent years, with the rapid development of nanomaterial preparation and characterization technologies, optical probes based on nanomaterials have great application prospects in the fields of biosensing, bioimaging and biodetection due to their unique optical properties and small size. received widespread attention. Optical probes of various structures and materials emerge in endlessly, which greatly improve the detection sensitivity and detection range, and effectively improve the research methods in the fields of biology and chemistry. [0003] Among optical detection technologies, fluorescence detection technology is fast and simple, and has been widely used in biosensing, drug development, tumor diagnosis and treatment and other fields. Ho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 王著元陈鹏崔一平
Owner SOUTHEAST UNIV
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