Method for detecting low-concentration mercury ions based on DNA modified SERS substrate

A technology of DNA molecules and mercury ions, which is applied in the preparation of test samples, Raman scattering, material excitation analysis, etc., can solve the problems of unable to generate Raman signals and directly measure metal ions, etc., and achieve strong SERS enhancement effect, Good uniformity in shape and particle size, and the effect of reducing interference

Active Publication Date: 2015-03-25
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

However, metal ions, including mercury ions, do not generate Raman signals by themselves, so this spectroscopic technique cannot directly measure metal ions

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  • Method for detecting low-concentration mercury ions based on DNA modified SERS substrate
  • Method for detecting low-concentration mercury ions based on DNA modified SERS substrate
  • Method for detecting low-concentration mercury ions based on DNA modified SERS substrate

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Embodiment Construction

[0022] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0023] see figure 2 , a method for detecting low-concentration mercury ions based on a DNA-modified SERS substrate of the present invention, comprising the following steps:

[0024] (1) Uniform gold-coated silica nanoparticles were prepared by a step-by-step method, and the core-shell nanoparticles were immobilized on a quartz glass plate using a coupling agent to construct a SERS substrate.

[0025] Specifically include:

[0026] 1a: First prepare silica nanoparticles.

[0027] 1b: Synthesizing Au gold seeds, specifically synthesizing 1-3nm Au gold seeds.

[0028] 1c: Preparation of SiO 2 Au core-shell nanop...

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Abstract

The invention discloses a method for detecting low-concentration mercury ions based on a DNA modified SERS substrate. The method comprises the following steps: preparing uniform gold-covered silicon dioxide nano particles by a fractional step method and fixing nano particles with a core-shell structure on a quartz glass sheet by a coupling agent to construct an SERS substrate; modifying single-chain DNA molecules on the surfaces of gold shell layers of the gold-covered silicon dioxide nano particles through sulfydryls; immersing the prepared DNA aptamer modified SERS substrate into a solution containing the mercury ions; washing with de-ionized water; by taking an SERS signal specific value of a G basic group and an A basic group as spectrum standard measurement of DNA at a metal surface state, realizing quantitative and specific detection on the mercury ions by analyzing a characteristic Raman peak and the change of the specific value. Compared with the prior art, the method has the following advantages that the sensitivity of the method is high, the detection lower limit is about 50nM, the measurement operation is simple, the cost is low, and the real-time and rapid measurement can be realized.

Description

technical field [0001] The invention relates to a method for detecting low-concentration mercury ions based on a DNA-modified SERS substrate. Background technique [0002] Mercury ion is a common pollutant in the water environment, causing serious harm to human health and ecological environment. Mercury ions, especially methylmercury, can accumulate in the human body through the food chain, causing permanent damage to the central nervous system, digestive system and kidneys and causing various chronic diseases. Methylmercury is the water-soluble mercury ion (Hg 2+ ) from methylation. Therefore, the quantitative and specific detection of mercury ions in the water environment is a problem that everyone has been concerned about. The general methods for detecting mercury ions are often not high in sensitivity or specificity, and are inconvenient to operate. Recently, people use Thymine-Hg 2+ -Thymine (T-Hg 2+ The coordination chemistry of -T) has designed some new detectio...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28
Inventor 黄青鲁逸林
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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