Method for detecting low-concentration mercury ions based on DNA modified SERS substrate

A technology of DNA molecules and mercury ions, which is applied in the preparation of test samples, Raman scattering, material excitation analysis, etc., can solve the problems of unable to generate Raman signals and directly measure metal ions, etc., and achieve strong SERS enhancement effect, Good uniformity in shape and particle size, and the effect of reducing interference
CN104458704AActive Publication Date: 2015-03-25HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
Publication Date
2015-03-25

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Abstract

The invention discloses a method for detecting low-concentration mercury ions based on a DNA modified SERS substrate. The method comprises the following steps: preparing uniform gold-covered silicon dioxide nano particles by a fractional step method and fixing nano particles with a core-shell structure on a quartz glass sheet by a coupling agent to construct an SERS substrate; modifying single-chain DNA molecules on the surfaces of gold shell layers of the gold-covered silicon dioxide nano particles through sulfydryls; immersing the prepared DNA aptamer modified SERS substrate into a solution containing the mercury ions; washing with de-ionized water; by taking an SERS signal specific value of a G basic group and an A basic group as spectrum standard measurement of DNA at a metal surface state, realizing quantitative and specific detection on the mercury ions by analyzing a characteristic Raman peak and the change of the specific value. Compared with the prior art, the method has the following advantages that the sensitivity of the method is high, the detection lower limit is about 50nM, the measurement operation is simple, the cost is low, and the real-time and rapid measurement can be realized.
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Description

technical field

[0001] The invention relates to a method for detecting low-concentration mercury ions based on a DNA-modified SERS substrate. Background technique

[0002] Mercury ion is a common pollutant in the water environment, causing serious harm to human health and ecological environment. Mercury ions, especially methylmercury, can accumulate in the human body through the food chain, causing permanent damage to the central nervous system, digestive system and kidneys and causing various chronic diseases. Methylmercury is the water-soluble mercury ion (Hg 2+ ) from methylation. Therefore, the quantitative and specific detection of mercury ions in the water environment is a problem that everyone has been concerned about. The general methods for detecting mercury ions are often not high in sensitivity or specificity, and are inconvenient to operate. Recently, people use Thymine-Hg 2+ -Thymine (T-Hg 2+ The coordination chemistry of -T) has designed some new detectio...

Claims

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