The invention discloses a directional osteogenesis induction
skull patch based on an MXene dual-
electrode plate and a pulsed direct-
current electric field and a preparation method of the directional osteogenesis induction
skull patch. The method specifically comprises the following steps: 1) performing intercalation on MXene by using a
bacterial cellulose solution as an intercalation agent, and combining a layer-by-layer blade
coating and laminating process to obtain a composite MXene
electrode plate; (2) preparing a porous GelMA pre-solution loaded with
bone marrow mesenchymal stem cells; 3)
cutting the composite MXene
electrode plate into a proper shape to obtain an upper-layer electrode plate and a lower-layer electrode plate, tiling the lower-layer electrode plate as a substrate, dispensing a
cell-loaded porous GelMA pre-solution on the surface of the lower-layer electrode plate, covering the upper-layer electrode plate, and performing gelation by combining lateral
UV curing to obtain a double-layer electrode plate composite gel material; and 4) carrying out in-vitro culture on the double-layer electrode plate composite gel material for several days, including electrical stimulation culture and non-electrical stimulation culture, and then stripping the upper electrode plate and the lower electrode plate to obtain the
skull patch. According to the skull patch, in-vivo
skull defect repair can be achieved, and a brand-new skull regeneration and repair strategy is created.